Abstract:
:MicroRNAs are small post-transcriptional regulators that modulate gene expression by directly interacting with their target transcripts. Since the interaction between miRNAs and target mRNAs does not require a perfect match, one single miRNA can influence the expression of several genes and lead to a very broad array of functional consequences. Recently, we identified miR-125a-3p as a new regulator of oligodendrocyte development, showing that its over-expression is associated to impaired oligodendrocyte maturation. However, whether and how miR-125a-3p over-expression is causally related to oligodendrocyte maturation is still obscure, as well as the pathways responsible for this effect. To shed light on this issue and to identify the underlying molecular mechanisms, we determined the transcriptomic profile of miR-125a-3p over-expressing oligodendrocytes and, by means of two complementary bioinformatic approaches, we have identified pathways and biological processes consistently modulated by miR-125a-3p alteration. This analysis showed that miR-125a-3p is involved in the regulation of cell-cell interactions and Wnt signaling. By means of pathway-focused PCR arrays, we confirmed that miR-125a-3p induces changes in the expression of several genes encoding for adhesion molecules and gap junctions, which play key roles in oligodendrocytes after exposure to pathological demyelinating stimuli. Moreover, the expression changes of different Wnt targets suggest an over-activation of this pathway. Globally, our studies show that miR-125a-3p over-expression can alter signaling pathways and biological processes essential for myelin formation in oligodendrocytes, suggesting that alteration of miR-125a-3p levels may contribute to impairing oligodendrocyte maturation in demyelinating diseases.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Marangon D,Abbracchio MP,Lecca Ddoi
10.1007/s10571-020-00836-zsubject
Has Abstractpub_date
2021-01-01 00:00:00pages
105-114issue
1eissn
0272-4340issn
1573-6830pii
10.1007/s10571-020-00836-zjournal_volume
41pub_type
杂志文章abstract::Perinatal hypoxia can lead to multiple chronic neurological deficits, e.g., mental retardation, behavioral abnormalities, and epilepsy. Levetiracetam (LEV), 2S-(2-oxo-1-pyrrolidiny1) butanamide, is an anticonvulsant drug with proven efficiency in treating patients with focal and generalized seizures. Rats were underwe...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-019-00676-6
更新日期:2019-07-01 00:00:00
abstract::(1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of loc...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-007-9148-y
更新日期:2007-09-01 00:00:00
abstract::1. Synaptic vesicles (SVs) mediate fast regulated secretion of classical neurotransmitters. In order to perform their task SVs rely on a restrict set of membrane proteins. The mechanisms responsible for targeting these proteins to the SV membrane are still poorly understood. 2. Likewise, little is known about the intr...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1021884319363
更新日期:2002-12-01 00:00:00
abstract::1. Two giant peptidergic neurons, VD1 and RPD2, of the visceral ganglion and right parietal ganglion of Lymnaea stagnalis, respectively, play an important role in the modulation of complex physiological and behavioral adjustments that occur as a result of changes in O2 availability. 2. By cDNA cloning, we have identif...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00735369
更新日期:1993-04-01 00:00:00
abstract::Secretory phospholipases A2 (sPLA2) form a diverse family of enzymes involved in physiologic and pathologic processes. Common among all sPLA2 is the ability to cleave acyl groups of phospholipids at C2 of the glycerol backbone, thereby releasing fatty acid and a lysophospholipid. Several sPLA2 have been cloned and cha...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-005-8221-7
更新日期:2005-11-01 00:00:00
abstract::While light is the basic element for inducing vision and modulating circadian rhythms, excessive light has been reported to have a negative effect on the survival of various types of retinal cells. Among them photoreceptors and retinal pigment epithelial (RPE) cells degeneration after light exposure is widely observed...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-020-00819-0
更新日期:2020-11-01 00:00:00
abstract::Autophagy is an essential degradation pathway in clearing abnormal protein aggregates in mammalian cells and is responsible for protein homeostasis and neuronal health. Several studies have shown that autophagy deficits occurred in early stage of Alzheimer's disease (AD). Autophagy plays an important role in generatio...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-016-0386-8
更新日期:2017-04-01 00:00:00
abstract::1. The aims of the present study were (a) to determine the identity of the G proteins with which the endothelin receptor interacts and whether this interaction is subtype specific and (b) to determine whether agonist exposure can result in specific coupling between the endothelin receptor and G proteins. 2. Coupling b...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1007010125316
更新日期:2000-06-01 00:00:00
abstract::1. Five drugs with the predominant pharmacologic effect of inhibiting the neuronal reuptake of serotonin are available worldwide for clinical use. This class of psychoactive drugs, known as selective serotonin reuptake inhibitors (SSRIs), is comprised of fluoxetine, sertraline, paroxetine, fluvoxamine, and citalopram....
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1006934807375
更新日期:1999-08-01 00:00:00
abstract::1. The growth inhibitory factor (GIF) is a 68-amino acid protein which is capable of inhibiting the growth of neuronal cells in vitro. 2. We have cloned and sequenced the 5'-flanking region of the mouse GIF gene, which spans from the transcriptional initiation site to the -1854 nucleotide. 3. This region contains sequ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1026369913405
更新日期:1997-04-01 00:00:00
abstract::The mammalian stress response is an integrated physiological and psychological reaction to real or perceived adversity. Glucocorticoids (GCs) are an important component of this response, acting to redistribute energy resources to both optimize survival in the face of challenge and restore homeostasis after the immedia...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-012-9801-y
更新日期:2012-02-01 00:00:00
abstract::1. Neurotrophins and serotonin have both been implicated in the pathophysiology of depression and in the mechanisms of antidepressant treatments. 2. Brain-derived neurotrophic factor (BDNF) influences the growth and plasticity of serotonergic (5-HT) neurons via the activation of trkB receptor. 3. Transgenic mice overe...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-005-8468-z
更新日期:2005-09-01 00:00:00
abstract:AIMS:Treatments that increase acetylcholine release from brain slices decrease the synthesis of phosphatidylcholine by, and its levels in, the slices. We examined whether adding cytidine or uridine to the slice medium, which increases the utilization of choline to form phospholipids, also decreases acetylcholine levels...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-006-9004-5
更新日期:2006-07-01 00:00:00
abstract::The intrauterinal development in mammals represents a very sensitive period of life in relation to many environmental factors, including ionizing radiation (IR). The developing nervous system is particularly vulnerable to IR, and the consequences of exposure are of importance because of its potential health risks. The...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0144-8
更新日期:2015-01-01 00:00:00
abstract::1. The effects of radiofrequency lesions of the ventral noradrenergic bundle (VNB) on monoamine and metabolite concentrations in several discrete areas of the rat hypothalamus were examined. Monoamines and metabolites were analyzed utilizing high-performance liquid chromatography coupled with electrochemical detection...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00733792
更新日期:1987-12-01 00:00:00
abstract::We demonstrated synchronous oscillation of intracellular Ca2+ in cultured-mouse mid-brain neurons. This synchronous oscillation was thought to result from spontaneous and synchronous neural bursts in a synaptic neural network. We also examined the role of endogenous dopamine in neural networks showing synchronous osci...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/b:cemn.0000012724.79184.b6
更新日期:2004-02-01 00:00:00
abstract::The effects of the calcium channel blocker D-600 on the cation channels activated by acetylcholine (ACh) was studied in voltage-clamped Aplysia neurons by voltage-jump relaxation analysis. D-600 blocked the steady-state ACh current in a highly voltage-dependent manner, the degree of antagonism increasing with membrane...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00734714
更新日期:1983-12-01 00:00:00
abstract::1. The central nervous systems (CNS) of males and females differ in the control mechanisms for the release of gonadotropins from the anterior pituitary gland as well as the capacity to display sex specific behaviors. 2. In guinea pigs and monkeys, these differences are organized through the actions of prenatal androge...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1023/a:1022534019718
更新日期:1997-12-01 00:00:00
abstract::Ischemic preconditioning (IPC) represents an important adaptation of CNS to sub-lethal ischemia, which results in increased tolerance of CNS to the lethal ischemia. Ischemia-induced mitochondrial apoptosis is considered to be an important event leading to neuronal cell death after cerebral blood flow arrest. In presen...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9373-7
更新日期:2009-09-01 00:00:00
abstract::Levels of cyclic nucleotides and ornithine decarboxylase (ODC) activity were examined following the application of various kinds of stimuli to superior cervical sympathetic ganglia (SCG), nodose ganglia, and vagus nerve fibers excised from the rat. The level of cyclic GMP in the SCG rose rapidly to about 4.5- to 7.5-f...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF00710944
更新日期:1984-03-01 00:00:00
abstract::Parkinson's disease (PD) is a chronic progressive neurodegenerative movement disorder characterized by the selective loss of nigrostriatal dopaminergic neurons. However, the molecular pathways leading to the dopaminergic neuron degeneration have remained obscure until recently. Reports demonstrated that reduction of b...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-010-9523-y
更新日期:2010-08-01 00:00:00
abstract::We investigated the postnatal alterations of neurons, astrocyte, oligodendrocyte, and microglia in the mouse hippocampal CA1 sector and dentate gyrus under the same conditions using immunohistochemistry. Neuronal nuclei (NeuN), Glial fibrillary acidic protein (GFAP), 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPas...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9412-4
更新日期:2009-12-01 00:00:00
abstract::Matrix metalloproteinase-1 (MMP-1), a member of the matrix metalloproteinases family, plays an integral role in extracellular matrix degradation and has been reportedly involved in the regulation of the brain or spinal cord traumatic neurovascular remodeling. Although the critical involvement of MMP-1 in the metastasi...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-014-0090-5
更新日期:2014-11-01 00:00:00
abstract::Hypoxia in neonates can lead to biochemical and molecular alterations mediated through changes in neurotransmitters resulting in permanent damage to brain. In this study, we evaluated the changes in the receptor status of GABA(A) in the cerebral cortex and brainstem of hypoxic neonatal rats and hypoxic rats supplement...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-009-9485-0
更新日期:2010-05-01 00:00:00
abstract::1. Oxytocin and vasopressin secretion from the neurohypophysis (NHP) is evoked by strongly patterned bursts of action potentials. We studied excitation-secretion coupling in single isolated terminals of rat NHP using patch clamp and capacitance detection techniques. 2. The secretory response evoked by trains of depola...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1023/a:1022575126738
更新日期:1998-02-01 00:00:00
abstract::1. Photoreceptor terminals in the flies Musca domestica and Drosophila melanogaster have been reconstructed in three dimensions from serial EM to reveal the surface distributions of afferent tetrad synapses. 2. The terminals are cylindrical and surround two target cells; they have synaptic sites distributed along thei...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/BF02151904
更新日期:1996-12-01 00:00:00
abstract::The original version of the article unfortunately contained an error in the unit of the protein concentrations under 'Stereotactic Intraparenchymal Injections' subsection in 'Methods' section. ...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10571-020-00872-9
更新日期:2020-07-01 00:00:00
abstract::To clarify the role of angiotensin II (Ang II) in the regulation of sensory signaling, we studied the effect of subpressor dose (150 ng/kg/min) of Ang II on pain-related behavior in relation with neuronal injury and activation of satellite glial cells (SGCs) in the dorsal root ganglia (DRGs) after chronic constriction...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9934-7
更新日期:2013-07-01 00:00:00
abstract::Both sex and steroid hormones are important to consider in human ischemic stroke and its experimental models. Stroke initiates a cascade of changes that lead to neural cell death, but also activates endogenous protective processes that counter the deleterious consequences of ischemia. Steroids may be part of these cer...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章,评审
doi:10.1007/s10571-018-0627-0
更新日期:2019-05-01 00:00:00
abstract::As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (S...
journal_title:Cellular and molecular neurobiology
pub_type: 杂志文章
doi:10.1007/s10571-013-9935-6
更新日期:2013-07-01 00:00:00