Abstract:
:(1) Axons contain numerous mRNAs and a local protein synthetic system that can be regulated independently of the cell body. (2) In this study, cultured primary sympathetic neurons were employed, to assess the effect of local protein synthesis blockade on axon viability and mitochondrial function. (3) Inhibition of local protein synthesis reduced newly synthesized axonal proteins by 65% and resulted in axon retraction after 6 h. Acute inhibition of local protein synthesis also resulted in a significant decrease in the membrane potential of axonal mitochondria. Likewise, blockade of local protein transport into the mitochondria by transfection of the axons with Hsp90 C-terminal domain decreased the mitochondrial membrane potential by 65%. Moreover, inhibition of the local protein synthetic system also reduced the ability of mitochondria to restore axonal levels of ATP after KCl-induced depolarization. (4) Taken together, these results indicate that the local protein synthetic system plays an important role in mitochondrial function and the maintenance of the axon.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Hillefors M,Gioio AE,Mameza MG,Kaplan BBdoi
10.1007/s10571-007-9148-ysubject
Has Abstractpub_date
2007-09-01 00:00:00pages
701-16issue
6eissn
0272-4340issn
1573-6830journal_volume
27pub_type
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