Abstract：
:The hypothesis that peripheral, skeletal muscle tissue contains a trophic factor supporting central neurons has recently been investigated in vitro by supplementing the culture medium of spinal cord neurons with muscle extracts and fractions of extract. We extended these studies asking whether or not a trophic factor is present in peripheral nerves, the connecting link between muscle and central neurons via which factors may be translocated from muscle to neurons by the retrograde transport system. Lumbar, 8-day-old chick spinal cords were dissociated into single cells and then cultured in the presence of peripheral nerve extract. Cytosine arabinoside was added to inhibit proliferation of nonneuronal cells. In the presence of nerve extract, spinal cord neurons survived for more than a month, extended numerous neurites, and showed activity of choline acetyltransferase. In the absence of extract, neurons attached and survived for a few days but then died subsequently in less than 10 days. Neurite outgrowth did not occur in the absence of extract. Withdrawal of extract from the medium of established neuronal cultures caused progressive loss of both cells and neurites. Other tissues also contained neuron supporting activity but less than that found in nerve extract. These studies indicate that peripheral nerves contain relatively high levels of spinal cord neuron-directed trophic activity, suggesting translocation of neurotrophic factor from muscle to central target neurons. The neurotrophic factor has long-term (weeks) effects, whereas short-term (days) survival is factor independent.
journal_name
Cell Mol Neurobioljournal_title
Cellular and molecular neurobiologyauthors
Popiela H,Porter T,Beach RL,Festoff BWdoi
10.1007/BF00710943subject
Has Abstractpub_date
1984-03-01 00:00:00pages
67-77issue
1eissn
0272-4340issn
1573-6830journal_volume
4pub_type
杂志文章abstract：:Cell cultures are characterized by their simplicity, controllability, and ability to provide detailed basic information on how a particular cell population responds to specific stimuli or insult. These characteristics led to their extensive application in the study of molecular interactions and represent a valuable to...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-017-0483-3
更新日期：2017-11-01 00:00:00
abstract：:The perineurium serves as a selective, metabolically active diffusion barrier in the peripheral nervous system, which is composed of perineurial cells joined together by tight junctions (TJs). Not only are these junctions known to play an essential role in maintaining cellular polarity and tissue integrity, but also l...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-020-00978-0
更新日期：2020-10-07 00:00:00
abstract：:1. Although the cellular and molecular mechanisms of the anticonvulsant action of gabapentin (GBP) remain incompletely described, in vitro studies have shown that GBP binds to the alpha2delta subunit of the high voltage-activated (HVA) Ca2+ channels. 2. In this report, we analyzed the effects of GBP on the functional ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1019865822069
更新日期：2002-04-01 00:00:00
abstract：:1. We describe here the alterations in the nociceptive sensitivity of Swiss CD1 mice receiving an intraplantar (i.pl.) administration of XC Rous sarcoma-virus-transformed rat fibroblasts (XC cells). 2. Histological studies reveal that XC cells remain at the injection site 2-3 weeks after implantation, a time at which ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/b:cemn.0000018621.58328.ea
更新日期：2004-04-01 00:00:00
abstract：:1. Transient forebrain ischemia in adult rats, induced by 10 min of bilateral carotid occlusion and an arterial hypotension of 40 mmHg, caused substantial damage not only to CA-1 neurons in hippocampus but also to epithelial cells in lateral ventricle choroid plexus. 2. When transient forebrain ischemia was followed b...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1023/a:1007097622590
更新日期：2000-04-01 00:00:00
abstract：:1. Alzheimer's disease is characterized by the deposition in the brain of extracellular amyloid plaques and vascular deposits consisting mostly of amyloid beta-peptide (A beta). A beta, a polypeptide of 39-43 amino acids (M(r), approximately 4 kDa), is derived proteolytically from a family of proteins of 695-770 amino...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF02088713
更新日期：1994-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00711580
更新日期：1993-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00733792
更新日期：1987-12-01 00:00:00
abstract：AIM:Poly (ADP-ribose) polymerase (PARP) is a nuclear repair enzyme whose role is widely depicted in various physiological and pathological processes. In the present study, we wanted to check the status of PARP and the role of various cell death proteases involved in apoptotic and non-apoptotic forms of cell death durin...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9348-8
更新日期：2009-06-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9573-1
更新日期：2010-11-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1007125413308
更新日期：2001-02-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-008-9308-8
更新日期：2009-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9445-8
更新日期：2010-03-01 00:00:00
abstract：:Reeler heterozygous mice (reln+/-) are seemingly normal but haplodeficient in reln, a gene implicated in autism. Structural/neurochemical alterations in the reln+/- brain are subtle and difficult to demonstrate. Therefore, the usefulness of these mice in translational research is still debated. As evidence implicated ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00683-7
更新日期：2019-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-011-9749-3
更新日期：2012-03-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s10571-018-0601-x
更新日期：2018-10-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-006-9134-9
更新日期：2007-06-01 00:00:00
abstract：:Abeta accumulation, a hallmark of Alzheimer's disease, promotes the disease progress in multiple facets. Abeta is formed through amyloidogenic cleavage pathway of amyloid precursor protein (APP). Production of Abeta can be decreased via activation of 5-HT2C receptor, which enhances alternative APP non-amyloidogenic cl...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-014-0138-6
更新日期：2015-04-01 00:00:00
abstract：:Alpha-synuclein (alpha-SYN) is one of the major components of intracellular fibrillary aggregates in the brains of a subset of neurodegenerative disorders. Although alpha-SYN expression has been found in developing mouse brain, a detailed distribution during mouse-embryonic development has not been made. Here we descr...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-009-9473-4
更新日期：2010-04-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-015-0287-2
更新日期：2016-08-01 00:00:00
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journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/BF00711228
更新日期：1988-12-01 00:00:00
abstract：:The P2X4 receptor (P2X4R) is a member of a family of ATP-gated cation channels that are composed of three subunits. Each subunit has two transmembrane (TM) domains linked by a large extracellular loop and intracellularly located N- and C-termini. The receptors are expressed in excitable and non-excitable cells and hav...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/s10571-010-9568-y
更新日期：2010-11-01 00:00:00
abstract：:1. 125I-Endothelin (ET)-1 binding to the rat anterior pituitary gland was saturable and single, with a Kd of 71 pM and a Bmax of 120 fmol/mg. 2. When 1.0 microM BQ-123 (ETA antagonist) was added to the incubation buffer, the binding parameters were 8.3 pM and 8.0 fmol/mg, whereas 10 nM sarafotoxin S6c (ETB agonist) ex...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1022557717481
更新日期：1998-08-01 00:00:00
abstract：:The ataxia-telangiectasia mutated (ATM) protein is regarded as the linchpin of cellular defenses to stress. Deletion of ATM results in strong oxidative stress and degenerative diseases in the nervous system. However, the role of ATM in neuronal ischemic preconditioning and lethal ischemic injury is still largely unkno...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-019-00773-6
更新日期：2020-07-01 00:00:00
abstract：:1. In the retinal inner nuclear layer of the majority of species, a dopaminergic neuronal network has been visualized in either amacrine cells or the so-called interplexiform cells. 2. Binding studies of retinal dopamine receptors have revealed the existence of both D1- as well D2-subtypes. The D1-subtype was characte...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1007/BF00711177
更新日期：1990-09-01 00:00:00
abstract：:As a novel cell cycle inhibitor, PHB2 controls the G1/S transition in cycling cells in a complex manner. Its aberrant expression is closely related to cell carcinogenesis. While its expression and role in peripheral nervous system lesion and repair were still unknown. Here, we performed an acute sciatic nerve crush (S...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-013-9935-6
更新日期：2013-07-01 00:00:00
abstract：:The degenerating muscle (dmu) mouse harbors a loss-of-function mutation in the Scn8a gene, which encodes the alpha subunit of the voltage-gated sodium channel (VGSC) Na(V)1.6. The distribution of c-Fos and c-Jun was examined in spinal and cranial motoneurons of the dmu mouse. In the cervical spinal cord, trigeminal mo...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-010-9498-8
更新日期：2010-07-01 00:00:00
abstract：:It is now well accepted that inflammatory responses in brain contribute to the genesis and evolution of damage in neurological diseases, trauma, and infection. Inflammatory mediators including cytokines, cell adhesion molecules, and reactive oxygen species including NO are detected in human brain and its animal models...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章,评审
doi：10.1023/a:1025084415833
更新日期：2003-10-01 00:00:00
abstract：AIM:In Alzheimer's disease (AD) it is well known that specific regions of the brain are particularly vulnerable to the pathologic insults of the disease. In particular, the hippocampus is affected very early in the disease and by end stage AD is ravaged by neurofibrillary tangles and senile plaques (i.e., the pathologi...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1023/a:1025063811290
更新日期：2003-10-01 00:00:00
abstract：:The pathogenesis of Alzheimer's disease (AD) has been strongly associated with the accumulation of amyloid beta (Aβ) peptides in brain, and immunotherapy targeting Aβ provides potential for AD prevention. A clinical trial in which AD patients were immunized with Aβ42 peptide was stopped when 6% of participants showed ...
journal_title：Cellular and molecular neurobiology
pub_type： 杂志文章
doi：10.1007/s10571-011-9680-7
更新日期：2011-08-01 00:00:00
