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Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus.

Abstract：

:The adult dentate gyrus produces new neurons that morphologically and functionally integrate into the hippocampal network. In the adult brain, most excitatory synapses are ensheathed by astrocytic perisynaptic processes that regulate synaptic structure and function. However, these processes are formed during embryonic or early postnatal development and it is unknown whether astrocytes can also ensheathe synapses of neurons born during adulthood and, if so, whether they play a role in their synaptic transmission. Here, we used a combination of serial-section immuno-electron microscopy, confocal microscopy, and electrophysiology to examine the formation of perisynaptic processes on adult-born neurons. We found that the afferent and efferent synapses of newborn neurons are ensheathed by astrocytic processes, irrespective of the age of the neurons or the size of their synapses. The quantification of gliogenesis and the distribution of astrocytic processes on synapses formed by adult-born neurons suggest that the majority of these processes are recruited from pre-existing astrocytes. Furthermore, the inhibition of astrocytic glutamate re-uptake significantly reduced postsynaptic currents and increased paired-pulse facilitation in adult-born neurons, suggesting that perisynaptic processes modulate synaptic transmission on these cells. Finally, some processes were found intercalated between newly formed dendritic spines and potential presynaptic partners, suggesting that they may also play a structural role in the connectivity of new spines. Together, these results indicate that pre-existing astrocytes remodel their processes to ensheathe synapses of adult-born neurons and participate to the functional and structural integration of these cells into the hippocampal network.

journal_name

Brain Struct Funct

journal_title

Brain structure & function

authors

Krzisch M,Temprana SG,Mongiat LA,Armida J,Schmutz V,Virtanen MA,Kocher-Braissant J,Kraftsik R,Vutskits L,Conzelmann KK,Bergami M,Gage FH,Schinder AF,Toni N

doi

10.1007/s00429-014-0768-y

subject

Has Abstract

pub_date

2015-07-01 00:00:00

pages

2027-42

issue

eissn

1863-2653

issn

1863-2661

journal_volume

220

pub_type

杂志文章

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Pre-existing astrocytes form functional perisynaptic processes on neurons generated in the adult hippocampus.