Abstract:
:Adverse effects of mercury on female reproduction are reported; however, its effect on myogenic activity of uterus and mechanism thereof is obscure. Present study was undertaken to unravel the mechanistic pathways of mercuric chloride (HgCl2)-induced myometrial contraction in rats. Isometric tension in myometrial strips of rats following in vitro exposure to HgCl2 was recorded using data acquisition system-based physiograph. HgCl2 produced concentration-dependent (10 nM-100 μM) uterotonic effect which was significantly (p < 0.05) reduced in Ca2+-free solution and inhibited in the presence of nifedipine (1 μM), a L-type Ca2+ channel blocker, thus suggesting the importance of extracellular Ca2+ and its entry through L-type calcium channels in HgCl2-induced myometrial contractions in rats. Cumulative concentration-response curve of HgCl2 was significantly (p < 0.05) shifted towards right in the presence of Y-27632 (10 μM), a Rho-kinase inhibitor, suggesting the involvement of Ca2+-sensitization pathway in mediating HgCl2-induced myometrial contraction. HgCl2-induced myometrial contraction was also significantly (p < 0.05) inhibited in the presence of methoctramine or para-fluoro-hexahydro-siladifenidol, a selective M2 and M3 receptor antagonists, respectively, which evidently suggest that mercury also interacts with M2 and M3 muscarinic receptors to produce myometrial contractions. U-73122 and GF-109203X, the respective inhibitors of PLC and PKC-dependent pathways, downstream to the receptor activation, also significantly (p < 0.05) attenuated the uterotonic effect of HgCl2 on rat uterus. Taken together, present study evidently reveals that HgCl2 interacts with muscarinic receptors and activates calcium signaling cascades involving calcium channels, Rho-kinase, protein kinase-C, and phospholipase-C pathways to exert uterotonic effect in rats. Graphical Abstract Graphical abstract depicting the mechanism of mercury-induced myometrial contraction in rats. M receptor: Muscarinic receptor; PIP2: phospho-inositol bisphosphate; PLC: phospholipase-C; DAG: diacyl glycerol; IP3: inositol triphosphate; IP3R: inositol triphosphate receptor; PKC; protein kinase-C; MLCP: myosin light chain phosphatise; MYPT: myosin phosphatase; SR: sarco-endoplasmic reticulum.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Koli S,Prakash A,Choudhury S,Mandil R,Garg SKdoi
10.1007/s12011-018-1379-xsubject
Has Abstractpub_date
2019-02-01 00:00:00pages
418-424issue
2eissn
0163-4984issn
1559-0720pii
10.1007/s12011-018-1379-xjournal_volume
187pub_type
杂志文章abstract::The synthesis, characterization, anti-hyperglycemic activity, oxidative DNA damage capacity, and acute toxicity of chromium(III) malate complex [Cr2(LMA)3] were described. [Cr2(LMA)3] was synthesized in a single-step reaction by chelating chromium(III) with L-malic acid in aqueous solution. Based on elemental analysis...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8916-6
更新日期:2011-11-01 00:00:00
abstract::Long-term diabetes mellitus can induce osteopenia and osteoporosis, an increase in the incidence of low-stress fractures, and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1322-1
更新日期:2018-12-01 00:00:00
abstract::This study aimed to investigate the effects of a combination of a dairy product fermented by lactobacilli (DFL) and galactooligosaccharides (GOS) on mineral balances in growing rats with hypochlorhydria induced by a proton pump inhibitor (PPI). Three-week-old male rats were assigned to receive one of six diets: a cont...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9681-0
更新日期:2013-06-01 00:00:00
abstract::Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model....
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9876-4
更新日期:2014-02-01 00:00:00
abstract::Neutron activation analysis (NAA) methodology was applied for the certification of eight trace elements (As, Co, Cr, Cu, Hg, Mn, Se, and Zn) in a plankton reference material (CRM 414) prepared by the BCR of the Commission of the European Communities. Average concentrations (micrograms/g dry wt) and standard deviations...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917365
更新日期:1994-10-01 00:00:00
abstract::The arsenic (As) hazardous quotient was estimated based on concentration of As in drinking water and scalp hair of male subjects of two age groups (n=360) consuming As contaminated water at different levels and non-contaminated drinking water. The total As concentrations in drinking water of less-exposed (LE) and high...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8933-5
更新日期:2011-11-01 00:00:00
abstract::Little is known about the influence of subchronic cadmium exposure on apoptosis in the immune organs of birds and the protective effects on apoptosis by selenium against cadmium. The aim of this study was to investigate the effect of subchronic cadmium exposure on nitric oxide and apoptosis in the immune organs of chi...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-0007-7
更新日期:2014-06-01 00:00:00
abstract::This work is a first attempt to determine the speciation of Cr in human plasma. With the aid of in vitro and in vivo 51Cr-labeled experiments, it was possible to develop the necessary biochemical techniques for the separation of the plasma proteins. Further work will use real samples, taking care to avoid contaminatio...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02992700
更新日期:1990-07-01 00:00:00
abstract::The effect of zinc deficiency on calmodulin function was investigated by assessing the in vivo activity of two calmodulin regulated enzymes, adenosine 3',5'-monophosphate (c-AMP) and guanosine 3',5'-monophosphate (c-GMP) phosphodiesterase (PDE) in several rat tissues. Enzymatic activities in brain, heart, and testis o...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02797137
更新日期:1988-08-01 00:00:00
abstract::Smoking is one of the major causes of mortality and numerous diseases, both directly and indirectly. The role of smoking as a significant risk factor is already known in several human diseases such as cardiovascular diseases, lung cancer, and chronic obstructive pulmonary disease. We aimed to compare the toxicity of h...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02537-6
更新日期:2021-01-20 00:00:00
abstract::Consumption of edible plants satisfies a significant part of human body needs in macro- and micronutrients while biologically active substances contain strong antioxidant properties and reduce the risk of a number of diseases. Balanced nutrition and design of personalized diets and treatment rely on the data on the co...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02345-y
更新日期:2020-08-19 00:00:00
abstract::The objective of the study was analysis of hair trace elements content in children with goiter living in Aktubinsk region. Children with goiter and age- and sex-adjusted controls were involved in the current study. Hair trace elements content was assessed using inductively coupled plasma mass spectrometry. Thyroid vol...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
doi:10.1007/s12011-016-0711-6
更新日期:2016-12-01 00:00:00
abstract::After considering the need for quality control in NAA, the concept of quality in NAA procedures themselves is discussed, and some important factors identified. Two approaches to improve quality are then described in more detail. The first concerns the unique ability of NAA using different isotopic reactions and differ...
journal_title:Biological trace element research
pub_type: 杂志文章,评审
doi:10.1007/BF02917356
更新日期:1994-10-01 00:00:00
abstract::In the present study, 15 different commercial tea brands sold in Khyber Pakhtunkhwa were collected from the markets. The samples were analyzed for the concentrations of ten selected heavy metals. The metal concentration showed a random distribution in all samples. The mean concentration of Cd, Cu, Mn, Pb, Zn, and Fe w...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02059-1
更新日期:2020-11-01 00:00:00
abstract::Selenium (Se) deficiency in the experimental models, Coturnix coturnix japonica and Corcyra cephalonica, resulted in impaired mitochondrial substrate oxidations and lowered thiol levels. Studies with respiratory inhibitors confirmed reduced mitochondrial electron transport enzyme activities, especially at cytochrome c...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784077
更新日期:1996-03-01 00:00:00
abstract::The metabolism of 203Hg-labeled methylmercury chloride (MeHg) has been studied in rabbits and hamsters. Rabbits were administered 1.6 mumols MeHgCl/kg bw intravenously, and hamsters 40 mumols/kg bw orally. Urine and feces were collected daily and groups of four animals killed after 1 h, 1 d, or 7 d. The concentration ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917256
更新日期:1989-07-01 00:00:00
abstract::This study evaluated the plasma levels of trace elements in children with chronic hepatitis B virus (HBV) infection and assessed whether they can be a factor that affects the response to interferon alpha (IFN-alpha) treatment. The study included 35 cases (ten girls, 25 boys) aged 3-13 years with chronic HBV infection ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-009-8507-6
更新日期:2010-06-01 00:00:00
abstract::Characterization of coffee terroirs is important to determine authenticity and provide confidence for consumers to select the right product. In this regard, Amhara Region, which is located at the northwestern part of Ethiopia, produces various local coffee types with distinct cup qualities. The coffees are, however, n...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01866-5
更新日期:2020-06-01 00:00:00
abstract::This study was designed to measure the effect of iron supplementation on antioxidant status in iron-deficient anemia, including the time for hemoglobin normalization and at the time of filling of iron body stores. The extent of plasma lipid peroxidation was evaluated by measuring the levels of malondialdehyde and glut...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:96:1-3:117
更新日期:2003-01-01 00:00:00
abstract::Selenium (Se) mainly performs its function through Se-containing proteins. Selenoprotein W (SelW), one member of the selenoprotein family, plays important roles in the normal function of the heart. To investigate the possible relationship between Se and SelW for the regulation of oxidative damage in chicken embryo myo...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-015-0529-7
更新日期:2016-06-01 00:00:00
abstract::Studies of effects of diazepam on liver parenchyma are very scanty. In this study, adult albino rats were treated with diazepam in two different doses (0.25 mg and 0.30 mg/kg body wt) daily for 30 and 60 d. Through light microscopy and electron microscopy, prenecrotic and necrotic changes were noted in the high-dose g...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02785292
更新日期:1997-06-01 00:00:00
abstract::Aluminum promotes osteoblast (OB) apoptosis. Apoptosis is induced by the disordered calcium homeostasis. Therefore, to investigate the relationship between Al-induced OB apoptosis and calcium homeostasis, calvarium OBs from neonatal rats (3-4 days) were cultured and exposed to 0.048-mg/mL Al(3+) or 0.048-mg/mL Al(3+) ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-015-0417-1
更新日期:2016-02-01 00:00:00
abstract::A study was conducted to determine the effect of dietary selenium (Se) concentration and source for broiler chickens on performance, nutrient digestibility, plasma Se, glutathione peroxidase (GPx) activity, and thiobarbituric acid reactive substances (TBARS). A total of 700 1-day-old broiler chicks were assigned to 7 ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-019-01801-8
更新日期:2020-04-01 00:00:00
abstract::The protective effects of caffeic acid on angiotensin-converting enzyme (ACE) and purinergic enzyme activities, as well as gluconeogenesis was investigated in iron-induced cardiotoxicity. Cardiotoxicity was induced in heart tissues harvested from healthy male SD rats by 0.1 mM FeSO4. Treatment was carried out by co-in...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02227-3
更新日期:2021-03-01 00:00:00
abstract::Trace element involvement in wounds left to heal by secondary intention needs clarification. We have previously reported faster healing of wounds following acute surgery compared with elective excision of pilonidal sinus disease. The effect of topical zinc on the closure of the excisional wounds was mediocre compared ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9658-z
更新日期:2013-06-01 00:00:00
abstract::The present study was undertaken to investigate the utility of cystatin C (CysC) as an early biomarker of cadmium (Cd)-induced renal injury. The study was carried out on 50 adult male individuals divided into five groups of 10 individuals as follows: control, welders, smoker welders, diabetic welders, and smoker diabe...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-014-9939-1
更新日期:2014-06-01 00:00:00
abstract::Preoperative diagnosis of papillary thyroid carcinoma (PTC) comprises numerous diagnostic procedures which are mostly applicable in tertiary institutions. Normal thyroid function depends on the presence of many trace elements and copper (Cu) and zinc (Zn) are some of those. The study is based on retrospective review o...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
doi:10.1007/s12011-014-0071-z
更新日期:2014-09-01 00:00:00
abstract::Strontium stimulates cartilage matrix formation in vitro. However, the mechanisms governing these effects have not yet been extensively reported. In this study, chondrocytes were isolated from rat articular cartilage by enzymatic digestion and cultured for 24-72 h with 1-5 mM strontium. We investigated the effects of ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9640-9
更新日期:2013-06-01 00:00:00
abstract::The present study investigated the role of kisspeptin-10 on reproductively significant trace elements in relation to testosterone release in male rabbits, Oryctolagus cuniculus. Groups of rabbits were exposed to single 1 μg kisspeptin dose (i.v., saphenous vein), while simultaneous groups were pretreated with a kisspe...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9720-x
更新日期:2013-08-01 00:00:00
abstract::Assessing the ability of a selenium (Se) sample to induce cellular glutathione peroxidase (GPx) activity in Se-deficient animals is the most commonly used method to determine Se bioavailability. Our goal is to establish a Se-deficient cell culture model with differential incorporation of Se chemical forms into GPx, wh...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-008-8097-8
更新日期:2008-07-01 00:00:00