Abstract:
:Interactions between proteins and drugs, which can lead to formation of stable drug-protein complexes, have important implications on several processes related to human health. These interactions can affect, for instance, free concentration, biological activity, and metabolism of the drugs in the blood stream. Here, we report on the UV-Visible spectroscopic investigation on the interaction of bovine serum albumin (BSA) with chlorophyll (Chl) in aqueous solution under physiological conditions. Binding constants at different temperatures--obtained by using the Benesi-Hildebrand equation--were found to be of the same order of magnitude (~10(4)M(-1)) indicating low affinity of Chl with BSA. We have found a hyperchromism, which suggested an interaction between BSA and Chl occurring through conformational changes of BSA caused by exposition of tryptophan to solvent. Films from BSA and Chl obtained at different Chl concentrations showed fractal structures, which were characterized by fractal dimension calculated from microscopic image analysis.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Gorza FD,Pedro GC,Trescher TF,da Silva RJ,Silva JR,de Souza NCdoi
10.1155/2014/872701subject
Has Abstractpub_date
2014-01-01 00:00:00pages
872701eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
杂志文章abstract::The insulin pathway is an anabolic pathway that controls, amongst other things, glucose homeostasis. It is an evolutionarily conserved pathway. Disruptions in insulin pathway functions can lead to diabetic states. Diabetes, a very common occurrence in modern life, afflicts a significant portion of the population of de...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/1451623
更新日期:2019-01-22 00:00:00
abstract:Background:Oxycodone is a widely used opioid analgesic, which is involved in cancer pain and non-cancer pain. This study is intended to understand the publication characteristics of oxycodone research field and assess the quality of pertinent articles from 1998 to 2017. Methods:Oxycodone-related publications from 1998...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/9096201
更新日期:2019-10-31 00:00:00
abstract::The aim of this study is to investigate the stress distributions of different restoration options for class II mesio-occluso-distal (MOD) cavities. A class II MOD cavity with proximal box gingival floor 1 mm below cementoenamel junction was designed in a mandibular first molar tooth model. 3D finite-element analysis (...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7435054
更新日期:2020-12-15 00:00:00
abstract::In response to cytokine signalling and other factors, CD4-positive T lymphocytes differentiate into distinct populations that are characterized by the production of certain cytokines and are controlled by different master transcription factors. The spectrum of such populations, which was initially limited to Th1 and T...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/327470
更新日期:2015-01-01 00:00:00
abstract:Background:The well-known rubber hand paradigm induces an illusion by having participants feel the touch applied to a fake hand. In parallel, the kinesthetic mirror illusion elicits illusions of movement by moving the reflection of a participant's arm. Experimental manipulation of sensory inputs leads to emergence of t...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/6937328
更新日期:2017-01-01 00:00:00
abstract::Bacterial infections are the most common cause of purulent soft tissue inflammations in the head and neck area. These bacteria are also responsible for the majority of inflammatory complications after third molar removal. The key to success of antibacterial treatment in both cases is the use of an appropriate antibact...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2010453
更新日期:2019-10-07 00:00:00
abstract:Background:Glucagon-like peptide 1 (GLP-1) hormone is an incretin hormone that is secreted in the ileum and plays a role in the pancreas to increase insulin secretion, stimulate proliferation, and prevent pancreatic β-cell apoptosis. Currently, diabetes mellitus (DM) treatment based on GLP-1 work is being developed, fo...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/8724824
更新日期:2019-11-11 00:00:00
abstract::Endothelial barrier dysfunction, which is a serious problem that occurs in various inflammatory conditions, permits extravasation of serum components into the surrounding tissues, leading to edema formation and organ failure. Pigment epithelium-derived factor (PEDF), which is a major endogenous antagonist, has been im...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/791825
更新日期:2015-01-01 00:00:00
abstract::A role of pro- and antioxidants for reducing rectal cancer (RC) incidence in operative, preoperative, and postoperative treatments is still disputable and controversial. The redox state of venous blood and tissues of blood vessels of 60 patients with RC (T2-4N0-2M0G2) and 20 donors is studied by means of the conventio...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/4848652
更新日期:2018-08-13 00:00:00
abstract::DNA Primase Subunit 1 (PRIM1) is crucial for cancer development and progression. However, there remains a lack of comprehension concerning the clinical implication of PRIM1 in HCC. Here, aberrant expression of PRIM1 was identified in HCC according to available databases. The prognostic value of PRIM1 in patients prese...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9689312
更新日期:2020-08-22 00:00:00
abstract::Significant positive effects, particularly on psychological state in patients who completed the follow-up pulmonary rehabilitation programs, are indicated by a large number of studies. Yet, a remarkable proportion of selected patients drop out from these programs. In this study, we investigated existing differences on...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/401326
更新日期:2014-01-01 00:00:00
abstract::We encapsulated tumor necrosis factor-α (TNF-α), a major proinflammatory cytokine, into cholesteryl pullulan (CHP) to prepare TNF/CHP nanoparticles. In this report, we describe the immune-enhancing capability of the nanoparticles to act as a vaccine adjuvant. TNF/CHP nanoparticles showed excellent storage stability an...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/471468
更新日期:2015-01-01 00:00:00
abstract::Bone integrity abnormality and imbalance between bone formation by osteoblasts and bone resorption by osteoclasts are known to result in metabolic bone diseases such as osteoporosis. Silymarin-rich milk thistle extract (MTE) and its component silibinin enhanced alkaline phosphatase activity of osteoblasts but reduced ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/919374
更新日期:2013-01-01 00:00:00
abstract::Upon allergen challenge, DC subsets are recruited to target sites under the influence of chemotactic agents; however, details pertinent to their trafficking remain largely unknown. We investigated the kinetic profiles of blood and skin-infiltrating DC subsets in twelve atopic subjects receiving six weekly intradermal ...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/231036
更新日期:2014-01-01 00:00:00
abstract:Background:Spinal cord injury (SCI) is often associated with long-term impairments related to functional limitations in the sensorimotor system. The use of virtual reality (VR) technology may lead to increased motivation and engagement, besides allowing a wide range of possible tasks/exercises to be implemented in reha...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/7106951
更新日期:2019-11-13 00:00:00
abstract::Despite significant advances in the study of the molecular mechanisms altered in the development and progression of neurodegenerative diseases (NDs), the etiology is still enigmatic and the distinctions between diseases are not always entirely clear. We present an efficient computational method based on protein-protei...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/686505
更新日期:2014-01-01 00:00:00
abstract::The large-scale production of cardiomyocytes is a key step in the development of cell therapy and tissue engineering to treat cardiovascular diseases, particularly those caused by ischemia. The main objective of this study was to establish a procedure for the efficient production of cardiomyocytes by reprogramming mes...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/390789
更新日期:2013-01-01 00:00:00
abstract::The aim of this study was to investigate whether osteopontin (OPN) variants are associated with susceptibility to ankylosing spondylitis (AS) in a Chinese population. Polymorphisms at the 9175th position in exon 7 of OPN and rs17524488 were genotyped using direct sequencing in 186 unrelated AS patients and 188 ethnica...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/3458439
更新日期:2018-01-18 00:00:00
abstract::Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations at both systemic and...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/106902
更新日期:2015-01-01 00:00:00
abstract::Objectives. This study aimed to determine the feasibility of cochlear implantation for sensorineural hearing loss in patients with Waardenburg syndrome. Method. A retrospective chart review was performed on patients who underwent cochlear implantation at the University of Tokyo Hospital. Clinical classification, genet...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/2854736
更新日期:2016-01-01 00:00:00
abstract::Some degree of external root resorption is a frequent, unpredictable, and unavoidable consequence of orthodontic tooth movement mediated by odontoclasts/cementoclasts originating from circulating precursor cells in the periodontal ligament. Its pathogenesis involves mechanical forces initiating complex interactions be...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2016/4864195
更新日期:2016-01-01 00:00:00
abstract::External focus of attention (EFA) and internal focus of attention (IFA) represent commonly used strategies to instruct subjects during exercise. Several studies showed EFA to be more effective than IFA to improve motor performance and learning. To date the role of these strategies on motor performance during finger mo...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2017/2946465
更新日期:2017-01-01 00:00:00
abstract::Previous studies have shown the therapeutic properties of ginseng and ginsenosides on hyperactive and impulsive behaviors in several psychiatric diseases. Herein, we investigated the effect of Panax ginseng Meyer (PG) on hyperactive/impulsive behaviors in a manic-like animal model, sleep deprivation (SD) rats. Male ra...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/3636874
更新日期:2020-10-17 00:00:00
abstract::The present study evaluated the anti-inflammatory and analgesic effects of the superoxide dismutase mimetic agent tempol in superoxide anion-induced pain and inflammation. Mice were treated intraperitoneally with tempol (10-100 mg/kg) 40 min before the intraplantar injection of a superoxide anion donor, potassium supe...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/9584819
更新日期:2017-01-01 00:00:00
abstract::Aim. The aim of the study was to compare the incidence of "de novo" overactive bladder (OAB) after sling surgeries and Burch procedure and to analyze the effect of the preoperative bladder volume on the incidence of this condition. Methods. This prospective trial included 290 female patients with stress urinary incont...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2019/9515242
更新日期:2019-02-12 00:00:00
abstract::The Purkinje network is a specialized conduction system within the heart that ensures the proper activation of the ventricles to produce effective contraction. Its role during ventricular arrhythmias is less clear, but some experimental studies have suggested that the Purkinje network may significantly affect the gene...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/621034
更新日期:2015-01-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) accounts for a significant proportion of liver cancer, which has become the second most common cause of cancer-related mortality worldwide. To investigate the potential mechanisms of invasion and progression of HCC, bioinformatics analysis and validation by qRT-PCR were performed. We fou...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1431396
更新日期:2018-08-29 00:00:00
abstract::Several factors, including compressive load and knee kinematics, have been shown to influence wear. External knee moments (a surrogate for load) have recently been correlated with the medial and lateral wear scar areas of an unconstrained, PCL retaining knee design. Therefore, the purpose of this study was to determin...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/284513
更新日期:2015-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal condition primarily characterized by the selective loss of upper and lower motor neurons. At present, the diagnosis and monitoring of ALS is based on clinical examination, electrophysiological findings, medical history, and exclusion of confounding disorders. There is the...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/525097
更新日期:2014-01-01 00:00:00
abstract::The AT-hook transcription factor, AKNA, is a nuclear protein that affects a few physiological and pathological processes including cancer. Here, we investigated the role of AKNA in gastric cancer (GC). By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays, AKNA was found deregulat...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6726759
更新日期:2020-05-13 00:00:00