A partial cDNA clone for porcine glucosephosphate isomerase: isolation, characterization and use in detection of restriction fragment length polymorphisms.

Abstract:

:A cDNA library for porcine skeletal muscle was established in the vector pBR322. The library was screened with an oligonucleotide probe coding for a hexapeptide from glucosephosphate isomerase (Gpi). A positive clone with an insert of about 450 bp and restriction sites for PstI, BamHI and PvuII was isolated. A 362-bp PstI fragment was sequenced and shown to contain the codons for the hexapeptide as well as the remaining 29 amino acids of this Gpi peptide. The PstI fragment was used to probe pig genomic DNA. The restriction enzymes PvuII and SacI detected a set of polymorphisms with five bands, behaving as a set of insertion/deletion polymorphisms.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Davies W,Harbitz I,Hauge JG

doi

10.1111/j.1365-2052.1987.tb00763.x

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

233-40

issue

3

eissn

0268-9146

issn

1365-2052

journal_volume

18

pub_type

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