A high-resolution radiation hybrid map of porcine chromosome 6.

Abstract:

:A high-resolution comprehensive map was constructed for porcine chromosome (SSC) 6, where quantitative trait loci (QTL) for reproduction and meat quality traits have been reported to exist. A radiation hybrid (RH) map containing 105 gene-based markers and 15 microsatellite markers was constructed for this chromosome using a 3000-rad porcine/hamster RH panel. In total, 40 genes from human chromosome (HSA) 1p36.3-p22, 29 from HSA16q12-q24, 17 from HSA18p11.3-q12 and 19 from HSA19q13.1-q13.4 were assigned to SSC6. All primers for these gene markers were designed based on porcine gene or EST sequences, and the orthologous status of the gene markers was confirmed by direct sequencing of PCR products amplified from separate Meishan and Large White genomic DNA pools. The RH map spans SSC6 and consists of six linkage groups created by using a LOD score threshold of 4. The boundaries of the conserved segments between SSC6 and HSA1, 16, 18 and 19 were defined more precisely than previously reported. This represents the most comprehensive RH map of SSC6 reported to date. Polymorphisms were detected for 38 of 105 gene-based markers placed on the RH map and these are being exploited in ongoing chromosome wide scans for QTL and eventual fine mapping of genes associated with prolificacy in a Meishan x Large White multigenerational commercial population.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Cao H,Robinson JA,Jiang Z,Melville JS,Golovan SP,Jones MW,Verrinder Gibbins AM

doi

10.1111/j.1365-2052.2004.01161.x

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

367-78

issue

5

eissn

0268-9146

issn

1365-2052

pii

AGE1161

journal_volume

35

pub_type

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