A first-generation microsatellite linkage map of the Japanese quail.

Abstract:

:A linkage map of the Japanese quail (Coturnix japonica) genome was constructed based upon segregation analysis of 72 microsatellite loci in 433 F(2) progeny of 10 half-sib families obtained from a cross between two quail lines of different genetic origins. One line was selected for long duration of tonic immobility, a behavioural trait related to fearfulness, while the other was selected based on early egg production. Fifty-eight of the markers were resolved into 12 autosomal linkage groups and a Z chromosome-specific linkage group, while the remaining 14 markers were unlinked. The linkage groups range from 8 cM (two markers) to 206 cM (16 markers) and cover a total map distance of 576 cM with an average spacing of 10 cM between loci. Through comparative mapping with chicken (Gallus gallus) using orthologous markers, we were able to assign linkage groups CJA01, CJA02, CJA05, CJA06, CJA14 and CJA27 to chromosomes. This map, which is the first in quail based solely on microsatellites, is a major step towards the development of a quality molecular genetic map for this valuable species. It will provide an important framework for further genetic mapping and the identification of quantitative trait loci controlling egg production and fear-related behavioural traits in quail.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Kayang BB,Vignal A,Inoue-Murayama M,Miwa M,Monvoisin JL,Ito S,Minvielle F

doi

10.1111/j.1365-2052.2004.01135.x

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

195-200

issue

3

eissn

0268-9146

issn

1365-2052

pii

AGE1135

journal_volume

35

pub_type

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