SNP detection and genetic mapping of porcine genes encoding enzymes in hepatic metabolic pathways and evaluation of linkage with carcass traits.

Abstract:

:We have previously identified and mapped porcine expressed sequence tags (ESTs) derived from genes that are preferentially expressed in liver. The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in porcine genes encoding enzymes in hepatic metabolic pathways and use the SNPs for mapping. Furthermore, these genes, which are involved in utilization and partitioning of nutrients, were examined for their effects on carcass and meat quality traits by linkage analyses. In total, 100 ESTs were screened for SNPs by single strand conformation polymorphism analyses across a diverse panel of animals with a 36% success rate. Twelve of 36 polymorphic loci segregated in a three-generation Duroc x Berlin Miniature Pig (F2) resource population, the DUMI resource population, and were genetically mapped. Interval mapping of the corresponding chromosomes was performed to verify mapping of the genes within quantitative trait loci (QTL) regions detected in this resource population. QTL with genome-wide significance were detected in the vicinity of GNMT, ESTL147 and HGD. These loci therefore are positional candidate genes.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Ponsuksili S,Chomdej S,Murani E,Bläser U,Schreinemachers HJ,Schellander K,Wimmers K

doi

10.1111/j.1365-2052.2005.01351.x

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

477-83

issue

6

eissn

0268-9146

issn

1365-2052

pii

AGE1351

journal_volume

36

pub_type

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