Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii).

Abstract:

:Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to use E. caballus-derived microsatellite markers to provide parentage verification and additional valuable information to the captive management of E. przewalskii. The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genus.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Breen M,Downs P,Irvin Z,Bell K

doi

10.1111/j.1365-2052.1994.tb00530.x

subject

Has Abstract

pub_date

1994-12-01 00:00:00

pages

401-5

issue

6

eissn

0268-9146

issn

1365-2052

journal_volume

25

pub_type

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