Abstract:
:An MspI polymorphism was identified in intron 13 of the equine homologue of proto-oncogene c-kit (KIT) by comparing DNA sequences from horses with solid coat colour and horses homozygous for the tobiano spotting (To) gene. The allele associated with solid coat colour was designated KM0, while the allele associated with the tobiano pattern created an additional MspI restriction site and was designated KM1. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) studies using DNA from hair follicles demonstrated that all 129 of 129 tobiano patterned horses possessed the KM1 allele. However, three of 104 solid-coloured thoroughbred horses also possessed the KM1 allele. Therefore, while KM1 is strongly associated with the gene for To, the association is not absolute. However, this test appears more efficacious to identify putative homozygotes for To than current biochemical testing methods using albumin (Alb) and vitamin D binding protein (Gc) haplotypes.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Brooks SA,Terry RB,Bailey Edoi
10.1046/j.1365-2052.2002.00854.xsubject
Has Abstractpub_date
2002-08-01 00:00:00pages
301-3issue
4eissn
0268-9146issn
1365-2052pii
854journal_volume
33pub_type
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