Abstract:
:We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable discrimination between the B and non-B alleles of beta-casein.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Damiani G,Pilla F,Leone P,Cacciò Sdoi
10.1111/j.1365-2052.1992.tb00180.xsubject
Has Abstractpub_date
1992-01-01 00:00:00pages
561-5issue
6eissn
0268-9146issn
1365-2052journal_volume
23pub_type
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