Direct sequencing and bidirectional allele specific polymerase chain reaction of the bovine beta-casein B variant.

Abstract:

:We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable discrimination between the B and non-B alleles of beta-casein.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Damiani G,Pilla F,Leone P,Cacciò S

doi

10.1111/j.1365-2052.1992.tb00180.x

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

561-5

issue

6

eissn

0268-9146

issn

1365-2052

journal_volume

23

pub_type

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