Abstract:
:Comparative anchor tagged sequence (CATS) consensus primers from loci mapped to human chromosomes 9, 10, 20, and 22 have been used to amplify homologous loci in pigs. Of 53, CATS primers tested in pigs, only 23 yielded products homologous to the human locus (42% success). Ten loci were physically mapped (43% success rate for verified products, but only 19% for primers tested). Due to lack of polymorphism, linkage mapping was possible only for AMBP. Map locations were consistent with human/pig ZOO-FISH, except for ADRA1A, whose position is still equivocal in humans. These CATS primers have made very limited contributions to pig/human comparative gene mapping because of low efficiency of amplification of orthologous porcine product, frequent amplification from rodent template in a somatic hybrid panel and low level of polymorphism.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Lee JH,Zhang W,Moran Cdoi
10.1046/j.1365-2052.2001.00787.xsubject
Has Abstractpub_date
2001-10-01 00:00:00pages
313-5issue
5eissn
0268-9146issn
1365-2052pii
787journal_volume
32pub_type
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