Abstract:
:Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were successfully used to amplify the equivalent region in 469 American bison (Bison bison). In domestic cattle, alleles of DRB3 are assigned through a restriction fragment length polymorphism (RFLP) analysis of the patterns of fragment lengths observed after digestion with the restriction enzymes RsaI, BstYI and HaeIII. In bison, using the same procedure, the observed RFLP patterns provided evidence for the strong conservation of restriction sites previously reported in cattle.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Morris BG,Spencer MC,Stabile S,Dodd JNdoi
10.1111/j.1365-2052.1994.tb00409.xsubject
Has Abstractpub_date
1994-06-01 00:00:00pages
91-3eissn
0268-9146issn
1365-2052journal_volume
25 Suppl 1pub_type
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