Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibi-DRB3 gene in American bison (Bison bison).

Abstract:

:Polymerase chain reaction (PCR) primers specific to exon 2 of the bovine lymphocyte antigen (BoLA)-DRB3 gene were successfully used to amplify the equivalent region in 469 American bison (Bison bison). In domestic cattle, alleles of DRB3 are assigned through a restriction fragment length polymorphism (RFLP) analysis of the patterns of fragment lengths observed after digestion with the restriction enzymes RsaI, BstYI and HaeIII. In bison, using the same procedure, the observed RFLP patterns provided evidence for the strong conservation of restriction sites previously reported in cattle.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Morris BG,Spencer MC,Stabile S,Dodd JN

doi

10.1111/j.1365-2052.1994.tb00409.x

subject

Has Abstract

pub_date

1994-06-01 00:00:00

pages

91-3

eissn

0268-9146

issn

1365-2052

journal_volume

25 Suppl 1

pub_type

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