Genome-wide methylation study of diploid and triploid brown trout (Salmo trutta L.).

Abstract:

:The induction of triploidization in fish is a very common practice in aquaculture. Although triploidization has been applied successfully in many salmonid species, little is known about the epigenetic mechanisms implicated in the maintenance of the normal functions of the new polyploid genome. By means of methylation-sensitive amplified polymorphism (MSAP) techniques, genome-wide methylation changes associated with triploidization were assessed in DNA samples obtained from diploid and triploid siblings of brown trout (Salmo trutta). Simple comparative body measurements showed that the triploid trout used in the study were statistically bigger, however, not heavier than their diploid counterparts. The statistical analysis of the MSAP data showed no significant differences between diploid and triploid brown trout in respect to brain, gill, heart, liver, kidney or muscle samples. Nonetheless, local analysis pointed to the possibility of differences in connection with concrete loci. This is the first study that has investigated DNA methylation alterations associated with triploidization in brown trout. Our results set the basis for new studies to be undertaken and provide a new approach concerning triploidization effects of the salmonid genome while also contributing to the better understanding of the genome-wide methylation processes.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Covelo-Soto L,Leunda PM,Pérez-Figueroa A,Morán P

doi

10.1111/age.12287

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

280-8

issue

3

eissn

0268-9146

issn

1365-2052

journal_volume

46

pub_type

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