Abstract:
:The selection and use of animals with blood group 0 in the process of transplanting pig organs or tissues into humans can positively contribute to the control of acute immune rejection due to differences in blood groups. Exon-specific PCRs for the porcine blood group A transferase gene against genomic DNA from either blood group A or 0 animals resulted in the amplification failure of the A0 blood group gene exon 8 from blood group 0 animals. To characterize the genetic abnormality in the genome of blood group 0 animals, we screened bacterial artificial chromosome (BAC) clones from a Korean native pig BAC library which had the blood group 0 allele, and carried out shotgun sequencing. The analysis showed that the 0 allele has a large deletion between exon 7 of the A0 blood group gene and the neighbouring SURF6. We also showed that the ABO blood group antigens in humans and the A0 blood group antigens in pigs are coded by mutations within the orthologous glycosyltransferase gene. In addition, we developed a multiplex genotyping method for the porcine A0 blood group gene.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Nguyen DT,Choi H,Jo H,Kim JH,Dirisala VR,Lee KT,Kim TH,Park KK,Seo K,Park Cdoi
10.1111/j.1365-2052.2010.02152.xsubject
Has Abstractpub_date
2011-06-01 00:00:00pages
325-8issue
3eissn
0268-9146issn
1365-2052journal_volume
42pub_type
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