Abstract:
:The objectives of this study were to assign both microsatellite and gene-based markers on porcine chromosome X to two radiation hybrid (RH) panels and to develop a more extensive integrated map of SSC-X. Thirty-five microsatellite and 20 gene-based markers were assigned to T43RH, and 16 previously unreported microsatellite and 15 gene-based markers were added to IMpRH map. Of these, 30 microsatellite and 12 gene-based markers were common to both RH maps. Twenty-two gene-based markers were submitted to BLASTN analysis for identification of orthologues of genes on HSA-X. Single nucleotide polymorphisms (SNPs) were detected for 12 gene-based markers, and nine of these were placed on the genetic map. A total of 92 known loci are present on at least one porcine chromosome X map. Thirty-seven loci are present on all three maps; 31 loci are found on only one map. Location of 33 gene-based markers on the comprehensive map translates into an integrated comparative map that supports conservation of gene order between SSC-X and HSA-X. This integrated map will be valuable for selection of candidate genes for porcine quantitative trait loci (QTLs) that map to SSC-X.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
McCoard SA,Fahrenkrug SC,Alexander LJ,Freking BA,Rohrer GA,Wise TH,Ford JJdoi
10.1046/j.1365-2052.2002.00878.xsubject
Has Abstractpub_date
2002-06-01 00:00:00pages
178-85issue
3eissn
0268-9146issn
1365-2052pii
878journal_volume
33pub_type
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