The influence of extracellular matrix and prolactin on global gene expression profiles of primary bovine mammary epithelial cells in vitro.

Abstract:

:An in vitro bovine mammosphere model was characterized for use in lactational biology studies using a functional genomics approach. Primary bovine mammary epithelial cells cultured on a basement membrane, Matrigel, formed three-dimensional alveoli-like structures or mammospheres. Gene expression profiling during mammosphere formation by high-density microarray analysis indicated that mammospheres underwent similar molecular and cellular processes to developing alveoli in the mammary gland. Gene expression profiles indicated that genes involved in milk protein and fat biosynthesis were expressed, however, lactose biosynthesis may have been compromised. Investigation of factors influencing mammosphere formation revealed that extracellular matrix (ECM) was responsible for the initiation of this process and that prolactin (Prl) was necessary for high levels of milk protein expression. CSN3 (encoding kappa-casein) was the most highly expressed casein gene, followed by CSN1S1 (encoding alphaS1-casein) and CSN2 (encoding beta-casein). Eighteen Prl-responsive genes were identified, including CSN1S1, SOCS2 and CSN2, however, expression of CSN3 was not significantly increased by Prl and CSN1S2 was not expressed at detectable levels in mammospheres. A number of novel Prl responsive genes were identified, including ECM components and genes involved in differentiation and apoptosis. This mammosphere model is a useful model system for functional genomics studies of certain aspects of dairy cattle lactation.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Riley LG,Gardiner-Garden M,Thomson PC,Wynn PC,Williamson P,Raadsma HW,Sheehy PA

doi

10.1111/j.1365-2052.2009.01964.x

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

55-63

issue

1

eissn

0268-9146

issn

1365-2052

pii

AGE1964

journal_volume

41

pub_type

杂志文章
  • Evaluation of the genetic diversity and population structure of Chinese indigenous horse breeds using 27 microsatellite markers.

    abstract::We determined the genetic diversity and evolutionary relationships among 26 Chinese indigenous horse breeds and two introduced horse breeds by genotyping these animals for 27 microsatellite loci. The 26 Chinese horse breeds come from 12 different provinces. Two introduced horse breeds were the Mongolia B Horse from Mo...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2010.02067.x

    authors: Ling YH,Ma YH,Guan WJ,Cheng YJ,Wang YP,Han JL,Mang L,Zhao QJ,He XH,Pu YB,Fu BL

    更新日期:2011-02-01 00:00:00

  • Genome-wide genetic diversity of 'Nici', the DNA source for the CHORI-260 turkey BAC library and candidate for whole genome sequencing.

    abstract::Vertebrate whole genome sequence assembly can benefit from a priori knowledge of variability in the target genome, with researchers often selecting highly inbred individuals for sequencing. However, for most species highly inbred research lines are lacking, requiring the use of an outbred individual(s). Here we examin...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2008.01845.x

    authors: Chaves LD,Harry DE,Reed KM

    更新日期:2009-06-01 00:00:00

  • Association analysis between canine behavioural traits and genetic polymorphisms in the Shiba Inu breed.

    abstract::The relationships between behavioural trait data and the genotype of 15 polymorphisms in eight neurotransmitter-related genes were analysed in 77 dogs of the Shiba Inu breed, an indigenous Japanese dog. The data were obtained from a 26-item questionnaire on the dog's behaviour, distributed to the dog's owners, through...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2009.01888.x

    authors: Takeuchi Y,Kaneko F,Hashizume C,Masuda K,Ogata N,Maki T,Inoue-Murayama M,Hart BL,Mori Y

    更新日期:2009-10-01 00:00:00

  • Unequivocal identification of the equine Dcfmqr phenogroup.

    abstract::An alloimmune reagent has been produced that distinguishes the equine factor Df in the D phenogroup, cfmqr, from that occurring in cefmqr and dfklr. Using this reagent it has been possible to correctly genotype Dc, d, f, k, l, m, q and r positive cells without recourse to family data. ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1996.tb00476.x

    authors: Bell K,Colling DT

    更新日期:1996-04-01 00:00:00

  • Diagnostic value of a microsatellite DNA marker for copper toxicosis in West-European Bedlington terriers and incidence of the disease.

    abstract::Recently, linkage of a DNA microsatellite marker to inherited copper toxicosis has been reported in American Bedlington terrier families. Due to the fact that there is little exchange of breeding stock between the USA and Europe, it remains to be investigated whether in Europe the marker is informative and is linked w...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.1999.00451.x

    authors: Rothuizen J,Ubbink GJ,van Zon P,Teske E,van den Ingh TS,Yuzbasiyan-Gurkan V

    更新日期:1999-06-01 00:00:00

  • Twenty years of artificial directional selection have shaped the genome of the Italian Large White pig breed.

    abstract::In this study, we investigated at the genome-wide level if 20 years of artificial directional selection based on boar genetic evaluation obtained with a classical BLUP animal model shaped the genome of the Italian Large White pig breed. The most influential boars of this breed (n = 192), born from 1992 (the beginning ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12392

    authors: Schiavo G,Galimberti G,Calò DG,Samorè AB,Bertolini F,Russo V,Gallo M,Buttazzoni L,Fontanesi L

    更新日期:2016-04-01 00:00:00

  • Mapping and characterization of the DQ subregion of the ovine MHC.

    abstract::A map of the ovine MHC class II DQ subregion has been constructed from overlapping cosmid clones. This region consists of two loci linked on a linear tract of 130 kb DNA. Each locus consists of a DQA and a DQB gene in a tail-to-tail orientation. The genes in each locus are transcribed but only those designated DQ1 exp...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1994.tb00200.x

    authors: Wright H,Ballingall KT

    更新日期:1994-08-01 00:00:00

  • Definition of the USDA103 strain of channel catfish (Ictalurus punctatus).

    abstract::Although there are differences in performance between genetic groups of channel catfish, identification and management of these groups is difficult because catfish strains look alike and individuals cannot be tagged efficiently. Thus, US catfish producers have not been able to objectively identify fish from different ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2007.01586.x

    authors: Waldbieser GC,Wolters WR

    更新日期:2007-04-01 00:00:00

  • Development of microsatellite markers and comparative mapping for bovine chromosome 19.

    abstract::Previous research has mapped an ovulation rate quantitative trait locus (QTL) to bovine chromosome 19. In an effort to enhance comparative mapping information and develop additional markers for refined QTL mapping, microsatellite markers were developed in a targeted approach. A bovine bacterial artificial chromosome (...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.2002.00808.x

    authors: Kirkpatrick BW,Byla B,Kurar E,Warren WC

    更新日期:2002-02-01 00:00:00

  • Maternity validation using sire-only BovineSNP50 BeadChip data.

    abstract::Based on pairwise identity-by-state (IBS) distances and whole-genome SNP data, kinship was investigated in the Israeli Holstein population. A total of 789 bulls, including most of the artificial insemination sires in service since 1987, were genotyped by the BovineSNP50 BeadChip. This sample included up to five genera...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12062

    authors: Seroussi E,Glick G,Shirak A,Ezra E,Zeron Y,Ron M,Weller JI

    更新日期:2013-12-01 00:00:00

  • Genetic diversity within chicken populations from different continents and management histories.

    abstract::Intra-population variation was assessed in 1970 chickens from 64 populations using 29 autosomal microsatellites. On average, 95% of the loci were polymorphic across populations. In 1456 (c. 83%) of the 1763 combinations of populations and polymorphic loci, no departure from Hardy-Weinberg equilibrium was observed. On ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2007.01650.x

    authors: Granevitze Z,Hillel J,Chen GH,Cuc NT,Feldman M,Eding H,Weigend S

    更新日期:2007-12-01 00:00:00

  • Mitochondrial PEPCK: a highly polymorphic gene with alleles co-selected with Marek's disease resistance in chickens.

    abstract::The gene coding for the mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M), a pivotal component in gluconeogenesis from lactate via the Cori cycle, was highly polymorphic in strains of egg-type chickens (White Leghorn) of different origins. Based on MspI restriction fragment polymorphisms a total of seven allel...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.1998.295362.x

    authors: Li S,Zadworny D,Aggrey SE,Kuhnlein U

    更新日期:1998-10-01 00:00:00

  • A radiation hybrid map of bovine chromosome 24 and comparative mapping with human chromosome 18.

    abstract::We present herein a bovine chromosome 24 (BTA24) radiation hybrid (RH) map using 40 markers scored on a panel of 90 RHs. Of these markers, 29 loci were ordered with odds of at least 1000:1 in a framework map. An average retention frequency of 17.4% was observed, with relatively higher frequencies near the centromere. ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.2003.00996.x

    authors: Kurar E,Womack JE,Kirkpatrick BW

    更新日期:2003-06-01 00:00:00

  • Comparative transcriptome analysis reveals genes related to the yolk ratio of duck eggs.

    abstract::Yolk ratio is an important production index in the salted duck egg industry. Yolk constituents are deposited during development of follicles. However, the molecular mechanism responsible for different yolk ratios in laying ducks remains elusive. In this study, Shaoxing ducks laying eggs with different yolk ratios were...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12820

    authors: Du X,Ren JD,Xu XQ,Chen GH,Huang Y,Du JP,Tao ZR,Cai ZX,Lu LZ,Yang H

    更新日期:2019-10-01 00:00:00

  • Disclosing the origin and diversity of Omani cattle.

    abstract::Among all livestock species, cattle have a prominent status as they have contributed greatly to the economy, nutrition and culture from the beginning of farming societies until the present time. The origins and diversity of local cattle breeds have been widely assessed. However, there are still some regions for which ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2012.02399.x

    authors: Mahgoub O,Babiker HA,Kadim IT,Al-Kindi M,Hassan S,Al-Marzooqi W,Eltahir YE,Al-Abri MA,Al-Khayat A,Al-Sinani KR,Hilal Al-Khanjari H,Costa V,Chen S,Beja-Pereira A

    更新日期:2013-06-01 00:00:00

  • A single-base change in the tyrosine kinase II domain of ovine FGFR3 causes hereditary chondrodysplasia in sheep.

    abstract::Ovine hereditary chondrodysplasia, or spider lamb syndrome (SLS), is a genetic disorder that is characterized by severe skeletal abnormalities and has resulted in substantial economic losses for sheep producers. Here we demonstrate that a non-synonymous T>A transversion in the highly conserved tyrosine kinase II domai...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2005.01398.x

    authors: Beever JE,Smit MA,Meyers SN,Hadfield TS,Bottema C,Albretsen J,Cockett NE

    更新日期:2006-02-01 00:00:00

  • Restriction fragment length polymorphisms for growth hormone, prolactin, osteonectin, alpha crystallin, gamma crystallin, fibronectin and 21-steroid hydroxylase in cattle.

    abstract::Genomic DNAs from animals representing six breeds of cattle (Angus, Brahman, Hereford, Holstein, Jersey and Texas Longhorn) were screened with cloned gene probes in a search for restriction fragment length polymorphisms (RFLPs). Eleven RFLPs were identified using seven different probes: growth hormone, prolactin, oste...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1989.tb00866.x

    authors: Theilmann JL,Skow LC,Baker JF,Womack JE

    更新日期:1989-01-01 00:00:00

  • Quantitative trait loci for litter size and prenatal loss in a White Duroc x Chinese Erhualian resource population.

    abstract::To detect quantitative trait loci (QTL) for litter size related traits, the total number of born piglets (TNB), the number of born alive piglets (NBA), the number of stillborn piglets (NSB) and the number of mummies (NM) at the first parity were recorded in 299 F(2) sows in a White Duroc x Chinese Erhualian intercross...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2009.01931.x

    authors: Li K,Ren J,Xing Y,Zhang Z,Ma J,Guo Y,Huang L

    更新日期:2009-12-01 00:00:00

  • Joint report of the Third International Workshop on Lymphocyte Alloantigens of the Horse, Kennett Square, Pennsylvania, 25-27 April 1984.

    abstract::The Third International Workshop on Lymphocyte Alloantigens of the Horse was held on 25-27 April 1984 in Kennett Square, Pennsylvania. Twelve laboratories from five countries participated. The principal purpose of this Workshop was to determine the phenotypic and gene frequencies of the 10 equine lymphocyte antigens (...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1986.tb00730.x

    authors: Antczak DF,Bailey E,Barger B,Guerin G,Lazary S,McClure J,Mottironi VD,Symons R,Templeton J,Varewyck H

    更新日期:1986-01-01 00:00:00

  • Toward the most ideal case-control design with related and unrelated dogs in whole-exome sequencing studies.

    abstract::With the recent development of whole-exome sequencing enrichment designs for the dog, a novel tool for disease-association studies became available. The aim of disease-association studies is to identify one or a very limited number of putative causal variants or genes from the large pool of genetic variation. To maxim...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12400

    authors: Broeckx BJ,Coopman F,Verhoeven GE,De Keulenaer S,De Meester E,Bavegems V,Smets P,Van Ryssen B,Van Nieuwerburgh F,Deforce D

    更新日期:2016-04-01 00:00:00

  • Analysis of ROH patterns in the Noriker horse breed reveals signatures of selection for coat color and body size.

    abstract::Overlapping runs of homozygosity (ROH islands) shared by the majority of a population are hypothesized to be the result of selection around a target locus. In this study we investigated the impact of selection for coat color within the Noriker horse on autozygosity and ROH patterns. We analyzed overlapping homozygous ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12797

    authors: Grilz-Seger G,Druml T,Neuditschko M,Mesarič M,Cotman M,Brem G

    更新日期:2019-08-01 00:00:00

  • Unveiling introgression in bumblebee (Bombus terrestris) populations through mitogenome-based markers.

    abstract::The bumblebee, Bombus terrestris, is an important pollinator commercially used on a global scale. The exported subspecies B. t. terrestris has colonised diverse environments, in some cases displacing wild pollinators to the verge of local extinction. In this sense, the native Iberian subspecies B. t. lusitanicus may b...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12874

    authors: Cejas D,López-López A,Muñoz I,Ornosa C,De la Rúa P

    更新日期:2020-02-01 00:00:00

  • A genome-wide association study for the number of teats in European rabbits (Oryctolagus cuniculus) identifies several candidate genes affecting this trait.

    abstract::In the European rabbit (Oryctolagus cuniculus), a polytocous livestock species, the number of teats indirectly impacts the doe reproduction efficiency and, in turn, the sustainable production of rabbit meat. In this study, we carried out a genome-wide association study (GWAS) for the total number of teats in 247 Itali...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.13036

    authors: Bovo S,Schiavo G,Utzeri VJ,Ribani A,Schiavitto M,Buttazzoni L,Negrini R,Fontanesi L

    更新日期:2021-01-11 00:00:00

  • A transposable element insertion in APOB causes cholesterol deficiency in Holstein cattle.

    abstract::Cholesterol deficiency, a new autosomal recessive inherited genetic defect in Holstein cattle, has been recently reported to have an influence on the rearing success of calves. The affected animals show unresponsive diarrhea accompanied by hypocholesterolemia and usually die within the first weeks or months of life. H...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12410

    authors: Menzi F,Besuchet-Schmutz N,Fragnière M,Hofstetter S,Jagannathan V,Mock T,Raemy A,Studer E,Mehinagic K,Regenscheit N,Meylan M,Schmitz-Hsu F,Drögemüller C

    更新日期:2016-04-01 00:00:00

  • Identification and SNP association analysis of a novel gene in chicken.

    abstract::A novel gene that was predicted to encode a long noncoding RNA (lncRNA) transcript was identified in a previous study that aimed to detect candidate genes related to growth rate differences between Chinese local breed Gushi chickens and Anka broilers. To characterise the biological function of the lncRNA, we cloned an...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12387

    authors: Mei X,Kang X,Liu X,Jia L,Li H,Li Z,Jiang R

    更新日期:2016-02-01 00:00:00

  • Genetic diversity analysis of French goat populations reveals selective sweeps involved in their differentiation.

    abstract::After domestication 11 000 years ago in Asia Minor, the goat followed human migration to Europe and Asia. It was then introduced in Africa and is now raised all over the world. In this study, we exploited a dataset composed of 54 000 SNPs (Illumina goat DNA chip) to analyze the genetic diversity of 223 individuals bel...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12752

    authors: Oget C,Servin B,Palhière I

    更新日期:2019-02-01 00:00:00

  • Linkage of bilateral convergent strabismus with exophthalmus (BCSE) to BTA5 and BTA18 in German Brown cattle.

    abstract::Bilateral convergent strabismus with exophthalmus (BCSE) is a widespread inherited eye defect in several cattle populations. Its progressive condition often leads to blindness in affected cattle and decreases their usability. Furthermore, the German animal welfare laws prevent breeding with animals whose progeny are e...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2008.01771.x

    authors: Mömke S,Fink S,Wöhlke A,Drögemüller C,Distl O

    更新日期:2008-10-01 00:00:00

  • Intrageneric amplification of horse microsatellite markers with emphasis on the Przewalski's horse (E. przewalskii).

    abstract::Primer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the dom...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1994.tb00530.x

    authors: Breen M,Downs P,Irvin Z,Bell K

    更新日期:1994-12-01 00:00:00

  • A genome-wide association study reveals a QTL influencing caudal supernumerary teats in Holstein cattle.

    abstract::Supernumerary teats represent a common abnormality of the bovine udder. A genome-wide association study was performed based on the proportion of the occurrence of supernumerary teats in the daughters of 1097 Holstein bulls. The heritability of caudal supernumerary teats without mammary gland in this study was 0.604. T...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12215

    authors: Joerg H,Meili C,Ruprecht O,Bangerter E,Burren A,Bigler A

    更新日期:2014-12-01 00:00:00

  • Genome-wide identification of quantitative trait loci in a cross between Hampshire and Landrace I: carcass traits.

    abstract::We report the identification of quantitative trait loci (QTL) affecting carcass composition, carcass length, fat deposition and lean meat content using a genome scan across 462 animals from a combined intercross and backcross between Hampshire and Landrace pigs. Data were analysed using multiple linear regression fitt...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2005.01405.x

    authors: Karlskov-Mortensen P,Bruun CS,Braunschweig MH,Sawera M,Markljung E,Enfält AC,Hedebro-Velander I,Josell A,Lindahl G,Lundström K,von Seth G,Jørgensen CB,Andersson L,Fredholm M

    更新日期:2006-04-01 00:00:00