Abstract:
:Novel high-throughput deep sequencing technology has dramatically changed the study of the functional complexity of transcriptomes. Here, we report the first use of this technology for analysing the wide range of transcriptional changes in porcine adipose tissue from different breeds and different growth phases in a model of obesity. Digital gene expression (DGE) data sets were instrumental to verifying the predicted gene models. We obtained a sequencing depth of over 3 million tags per sample (lean, obese-a and obese-b). Tag mapping indicated expression of more than 76% of all genes represented in three transcript databases. We found the expression level of 1596 genes was significantly different between lean and obese-a library (P < 0.01). Among them, we found 84 genes expressed only in the obese-a library and 95 genes expressed only in the lean library. Moreover, the expression of 4403 genes was found to be remarkably different between the obese-a and obese-b library (P < 0.01); 642 of these were only expressed in obese-a, and 618 were only expressed in obese-b. When mapping to genes, it was found that the sense transcripts account for 67.42%, 68.65% and 66.61% of all clean tags in the lean, obese-a and obese-b libraries respectively. By comparison, the ratio of sense to antisense mapping of the total number of tags was approximately 6:1 for all libraries. This suggests that transcriptional regulation on the sense strand has a major role in adipose deposition, although a high number of antisense mapping events were also detected. We anticipated more than 20,000 different novel tags to be localized to the porcine genome. Among them, 799 different clean tags with a copy number of more than 1000 were detected. In conclusion, our deep sequencing analysis revealed a high degree of transcriptional complexity in the regulatory mechanisms of adipogenesis and resulted in the discovery and validation of new gene products in porcine adipose tissue.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Li XJ,Yang H,Li GX,Zhang GH,Cheng J,Guan H,Yang GSdoi
10.1111/j.1365-2052.2011.02240.xsubject
Has Abstractpub_date
2012-04-01 00:00:00pages
144-52issue
2eissn
0268-9146issn
1365-2052journal_volume
43pub_type
杂志文章相关文献
ANIMAL GENETICS文献大全abstract::Performance data for 164,046 Thoroughbreds entered in a race or official barrier trial in Australia were provided by Racing Information Services Australia. Analyses estimating the heritability for a range of racing performance traits using a single-trait animal model were performed using ASREML-R. Log of cumulative ea...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12234
更新日期:2015-02-01 00:00:00
abstract::In previous GWAS carried out in a Duroc commercial line (Lipgen population), we detected on pig chromosomes 3, 4 and 14 several QTL for gluteus medius muscle redness (GM a*), electric conductivity in the longissimus dorsi muscle (LD CE) and vaccenic acid content in the LD muscle (LD C18:1 n - 7), respectively. We have...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12864
更新日期:2020-02-01 00:00:00
abstract::The London strain of rainbow trout (Oncorhynchus mykiss) was created by interbreeding three other strains of rainbow trout and therefore was expected to have higher levels of genetic variation than other strains of rainbow trout. We examined 129 London strain rainbow trout from Indiana by allozyme electrophoresis to a...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.2001.00766.x
更新日期:2001-08-01 00:00:00
abstract::Amber (previously called X-Colour) is a yellow recessive coat colour observed in the Norwegian Forest Cat (NFC) population and apparently absent in other cat breeds. Until now, there has never been any scientific evidence of yellow recessive mutation (e) reported in the extension gene in Felidae. We sequenced the comp...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2009.01864.x
更新日期:2009-08-01 00:00:00
abstract::Forty-two microsatellite loci were analysed in 23 highly inbred chicken lines derived from Leghorn, Jungle Fowl, Fayoumi and Spanish breeds. Line-specific alleles among breeds and lines were detected. The band-sharing (BS) values were calculated and the proportion of shared alleles distances (Dps) were estimated. The ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.1999.00505.x
更新日期:1999-08-01 00:00:00
abstract::Presented herein is an updated sheep cytogenetic map that contains 452 loci (291 type I and 161 type II) assigned to specific chromosome bands or regions on standard R-banded ideograms. This map, which significantly extends our knowledge of the physical organization of the ovine genome, includes new assignments for 88...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2007.01598.x
更新日期:2007-06-01 00:00:00
abstract::There are two categories of immune responses - innate and adaptive immunity - both having polygenic backgrounds and a significant environmental component. In our study, adaptive immunity was represented by the specific antibody response toward keyhole limpet hemocyanin (KLH); innate immunity was represented by natural...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12280
更新日期:2015-06-01 00:00:00
abstract::Dexter cattle carry a genetic defect causing a dwarf phenotype in the heterozygotes (Dx+/-), while homozygotes (Dx+/+) are stillborn with extreme shortening of limbs and gross craniofacial defects and are described as 'bulldog' calves. The heterozygous phenotype has been likened to achondroplastic dwarfism in humans (...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1997.00068.x
更新日期:1997-02-01 00:00:00
abstract::The recent extension of genetic tools to the domestic cat, together with the serendipitous consequences of selective breeding, have been essential to the study of the genetic diseases that affect them. Cats are increasingly presented for veterinary surveillance and share many of human's heritable diseases, allowing th...
journal_title:Animal genetics
pub_type: 杂志文章,评审
doi:10.1111/age.12799
更新日期:2019-08-01 00:00:00
abstract::Almost half of all US households own a dog (Canis familiaris). Though these household pets can attack humans and other animals, they are also frequently victims of cruelty, neglect and theft. In human-oriented investigations, the tendency of domestic dogs to leave behind physical traces (such as hair) can serve as val...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12758
更新日期:2019-02-01 00:00:00
abstract::A high-resolution comprehensive map was constructed for porcine chromosome (SSC) 6, where quantitative trait loci (QTL) for reproduction and meat quality traits have been reported to exist. A radiation hybrid (RH) map containing 105 gene-based markers and 15 microsatellite markers was constructed for this chromosome u...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2004.01161.x
更新日期:2004-10-01 00:00:00
abstract::RNA editing is a post-transcription maturation process that diversifies genomically encoded information and can lead to transcriptome diversity. Thanks to next-generation sequencing technologies, a large number of editing sites have been identified in different species. Although this mechanism is well described in mam...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12818
更新日期:2019-10-01 00:00:00
abstract::The genetic differentiation and phylogenetic relationships of 18 indigenous goat populations from seven East Asian countries were analysed based on data obtained from 26 microsatellite DNA markers. The mean number of alleles (MNA) per population ranged from 2.5 to 7.6, with an average of 5.8. Genetic variability estim...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2012.02334.x
更新日期:2012-12-01 00:00:00
abstract::The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene. A single positive recombinant phage clone containing a 10.7-kb insert was isolated from a porcine genomic library, and a 4.2-kb fragment was subcloned and sequenced. The 4.2-kb fragment contained the e...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.1998.00273.x
更新日期:1998-02-01 00:00:00
abstract::The use of the AFLP (amplified fragment length polymorphism) technique for the characterization of highly inbred Iberian pig breed genotypes and the detection of strain-specific polymorphisms is demonstrated. Twelve different primer combinations were used on individual DNA samples from animals belonging to two black h...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.2000.00603.x
更新日期:2000-04-01 00:00:00
abstract::Toll-like receptor 3 (TLR3) has an important protective function against viral infection. The ability of an individual to respond properly to TLR ligands may be impaired by variants located in the TLR genes. By directly PCR sequencing four exons and their flanking sequence of chicken TLR3, a total of 50 nucleotide var...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2011.02244.x
更新日期:2012-06-01 00:00:00
abstract::An important aim in animal breeding is the improvement of growth and meat quality traits. Previous studies have demonstrated that genetic variants in the fat mass and obesity associated (FTO) gene have a relatively large effect on human obesity as well as on body composition in rodents and, more recently, in livestock...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12403
更新日期:2016-04-01 00:00:00
abstract::The stearoyl-CoA desaturase (delta-9-desaturase) gene encodes a key enzyme in the cellular biosynthesis of monounsaturated fatty acids. In our initial genome-wide association study (GWAS) of Chinese Holstein cows, 19 SNPs fell in a 1.8-Mb region (20.3-22.1 Mb) on chromosome 26 underlying the SCD gene and were highly s...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12432
更新日期:2016-08-01 00:00:00
abstract::A GWAS was performed using the genotypes obtained by PorcineSNP60 v2 BeadChip and 11 phenotypic traits (carcass lean meat percentage; backfat thickness; Longissimus thoracis muscle thickness; lightness; backfat thickness measured with caliper at the midline; meat pH measured at about 1 h post mortem and 24 h post mort...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.13013
更新日期:2020-12-01 00:00:00
abstract::Microsatellite polymorphisms are finding increasing use in genetics. In addition to the random isolation of microsatellite markers, such markers can also be developed from sequences already present in public domain databases. An advantage of public domain databases is that these microsatellites are known to be located...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1995.tb02636.x
更新日期:1995-04-01 00:00:00
abstract::Feed efficiency and growth are the most important traits in pig production, and very few genetic markers have been reported to be associated with feed efficiency. The suppressor of cytokine signalling-2 (encoded by SOCS2) is the main negative regulator of somatic growth, and the knockout of SOCS2 and naturally mutant ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2010.02107.x
更新日期:2011-04-01 00:00:00
abstract::Although there are differences in performance between genetic groups of channel catfish, identification and management of these groups is difficult because catfish strains look alike and individuals cannot be tagged efficiently. Thus, US catfish producers have not been able to objectively identify fish from different ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2007.01586.x
更新日期:2007-04-01 00:00:00
abstract::Extracellular superoxide dismutase (SOD3) is a major antioxidant enzyme that protects organs from damage by reactive oxygen species (ROS). In this study, the SOD3 gene was identified and characterized from the freshwater mussel Hyriopsis cumingii (Hc-SOD3). The cDNA sequence consists of 763 bp, encoding a protein of 2...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12059
更新日期:2013-12-01 00:00:00
abstract::The Neolithic introduction of domestic cattle into Europe was followed by differential adaptation, selection, migration and genetic isolation, leading ultimately to the emergence of specialized breeds. We have studied the differentiation of European cattle by amplified fragment length polymorphism (AFLP) fingerprintin...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2007.01554.x
更新日期:2007-02-01 00:00:00
abstract::Genetic diversity in the second domain exon of Atlantic salmon (Salmo salar) major histocompatibility complex (Mhc) class I was investigated in two dams and nine of their haploid offspring by means of polymerase chain reaction (PCR) and DNA sequence analysis. A similar study was also performed on nine diploid offsprin...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1997.00183.x
更新日期:1997-12-01 00:00:00
abstract::Body weight is a complex trait in cattle associated with commonly used commercial breeding measurements related to growth. Although many quantitative trait loci (QTL) for body weight have been identified in cattle so far, searching for genetic determinants in different breeds or environments is promising. Therefore, w...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12786
更新日期:2019-06-01 00:00:00
abstract::Four new alleles at the rabbit (Oryctolagus cuniculus) mannose-6-phosphate isomerase (E.C. 5.3.1.8, MPI) locus are proposed to account for phenotypes observed after starch gel electrophoresis and enzymatic staining of red cell lysates and tissues. Population data from various wild and domestic rabbit populations are p...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1995.tb02617.x
更新日期:1995-02-01 00:00:00
abstract::The Third International Workshop on Lymphocyte Alloantigens of the Horse was held on 25-27 April 1984 in Kennett Square, Pennsylvania. Twelve laboratories from five countries participated. The principal purpose of this Workshop was to determine the phenotypic and gene frequencies of the 10 equine lymphocyte antigens (...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1986.tb00730.x
更新日期:1986-01-01 00:00:00
abstract::A synthetic oligodeoxyribonucleotide probe (OAT36) comprising nine repeats of 5'GACA 3' and several enzymes were used to analyse cow, (Bos taurus) and buffalo (Bubalus bubalis) genomes and a number of monomorphic loci were detected in both the species. Different animals from the same species showed an almost 'similar'...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1993.tb00288.x
更新日期:1993-06-01 00:00:00
abstract::A candidate gene analysis of the microphthalmia-associated transcription factor (MITF) gene was used in an attempt to identify the genetic basis for a white-spotted coat color phenotype in the Asian swamp buffalo (Bubalus bubalis carabanensis). Ninety-three buffaloes-32 solid, 38 spotted and 23 white individuals-were ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12334
更新日期:2015-12-01 00:00:00