Isolation and genetic characterization of the porcine apolipoprotein E gene.

Abstract:

:The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene. A single positive recombinant phage clone containing a 10.7-kb insert was isolated from a porcine genomic library, and a 4.2-kb fragment was subcloned and sequenced. The 4.2-kb fragment contained the entire apo-E gene in addition to upstream and downstream sequences (GenBank accession no. 470240). The porcine apo-E gene is made up of four exons and three introns, and encodes a preapo-E protein comprised of a signal peptide of 18 amino acids and a mature protein of 299 amino acids. The porcine apo-E gene contains a (CG)13 microsatellite marker within intron three. This microsatellite is moderately polymorphic, and at least four alleles were evident at this locus among 10 animals from each of the Yorkshire, Hampshire, Landrace and Duroc breeds. Finally, localization of the porcine apo-E gene to chromosome 6 band q2.1 was determined by fluorescent in situ hybridization and confirmed by genetic linkage analysis.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Ramsoondar JJ,Rucker EB,Vasquez JC,Gallagher DS,Grimm DR,Lunney JK,Schook LB,Piedrahita JA

doi

10.1046/j.1365-2052.1998.00273.x

subject

Has Abstract

pub_date

1998-02-01 00:00:00

pages

43-7

issue

1

eissn

0268-9146

issn

1365-2052

journal_volume

29

pub_type

杂志文章
  • The genomic architecture of South African mutton, pelt, dual-purpose and nondescript sheep breeds relative to global sheep populations.

    abstract::South Africa has a diverse array of phenotypically distinct and locally adapted sheep breeds that have been developed for different production systems ranging from mutton to wool and pelt, and some dual-purpose and nondescript breeds kept by smallholder farmers. This study investigated genetic diversity, population ge...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12991

    authors: Dzomba EF,Chimonyo M,Snyman MA,Muchadeyi FC

    更新日期:2020-12-01 00:00:00

  • A single nucleotide polymorphism in suppressor of cytokine signalling-2 is associated with growth and feed conversion efficiency in pigs.

    abstract::Feed efficiency and growth are the most important traits in pig production, and very few genetic markers have been reported to be associated with feed efficiency. The suppressor of cytokine signalling-2 (encoded by SOCS2) is the main negative regulator of somatic growth, and the knockout of SOCS2 and naturally mutant ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2010.02107.x

    authors: Chen Y,Piper E,Zhang Y,Tier B,Graser HU,Luxford BG,Moran C

    更新日期:2011-04-01 00:00:00

  • Marker-derived phylogeny of European cattle supports demic expansion of agriculture.

    abstract::Using frequencies of 86 genes from 23 loci of blood group systems, blood and milk proteins, the genetic relationships among 14 cattle breeds including four native Balkan and four synthetic Balkan-Alp breeds were studied. The dendrogram and nonlinear map construction shows a consensus 'Balkan breed cluster', an 'Alp br...

    journal_title:Animal genetics

    pub_type: 历史文章,杂志文章

    doi:10.1111/j.1365-2052.1994.tb00399.x

    authors: Medjugorac I,Kustermann W,Lazar P,Russ I,Pirchner F

    更新日期:1994-06-01 00:00:00

  • Identification of copy number variations in three Chinese horse breeds using 70K single nucleotide polymorphism BeadChip array.

    abstract::Copy number variation (CNV), an essential form of genetic variation, has been increasingly recognized as one promising genetic marker in the analysis of animal genomes. Here, we used the Equine 70K single nucleotide polymorphism genotyping array for the genome-wide detection of CNVs in 96 horses from three diverse Chi...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12451

    authors: Kader A,Liu X,Dong K,Song S,Pan J,Yang M,Chen X,He X,Jiang L,Ma Y

    更新日期:2016-10-01 00:00:00

  • Altered expression of CYP17A1 and CYP19A1 in undescended testes of dogs with unilateral cryptorchidism.

    abstract::Cryptorchidism is the most common disorder of sex development in dogs and testosterone plays a crucial role in the inguinal phase of the testes descending into the scrotum. The molecular background of impaired testosterone synthesis in the testes of cryptorchid dogs is poorly elucidated. In this study, we analyzed the...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12977

    authors: Krzeminska P,Stachowiak M,Skrzypski M,Nowak T,Maslak A,Switonski M

    更新日期:2020-10-01 00:00:00

  • Maternity validation using sire-only BovineSNP50 BeadChip data.

    abstract::Based on pairwise identity-by-state (IBS) distances and whole-genome SNP data, kinship was investigated in the Israeli Holstein population. A total of 789 bulls, including most of the artificial insemination sires in service since 1987, were genotyped by the BovineSNP50 BeadChip. This sample included up to five genera...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12062

    authors: Seroussi E,Glick G,Shirak A,Ezra E,Zeron Y,Ron M,Weller JI

    更新日期:2013-12-01 00:00:00

  • T lymphocyte development and maturation in horses.

    abstract::Monoclonal antibodies specific for equine T lymphocyte subpopulations were produced and procedures for the continuous culture of equine lymphocytes were developed. These reagents and procedures were used to analyse the appearance, maturation and functions of T lymphocytes in normal horses and in T lymphocyte deficient...

    journal_title:Animal genetics

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2052.1988.tb00825.x

    authors: Perryman LE,Wyatt CR,Magnuson NS,Mason PH

    更新日期:1988-01-01 00:00:00

  • Assessment of DNA fingerprinting for determining genetic diversity in sheep populations.

    abstract::Genomic DNA, prepared from 12 animals from four sheep flocks, was digested with either HaeIII or HinfI and probed with three DNA fingerprinting probes. Mean DNA fingerprint band sharing and band frequency calculated for each flock were used to estimate genetic diversity. Each of the DNA fingerprinting systems showed t...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1993.tb00345.x

    authors: Hermans IF,Morris CA,Chambers GK,Towers NR,Jordan TW

    更新日期:1993-10-01 00:00:00

  • No detectable association of the ESR PvuII mutation with sow productivity in a Meishan x Large White F2 population.

    abstract::The polymorphism at the PvuII recognition site in the ESR gene showed no statistically significant association with sow productivity traits in a Meishan x Large White F2 population. Estimates of the effect on litter size were, however, in the opposite direction and statistically different from previously published est...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.2002.00889.x

    authors: Gibson JP,Jiang ZH,Robinson JA,Archibald AL,Haley CS

    更新日期:2002-12-01 00:00:00

  • IGF2 gene characterization and association with rib eye area in beef cattle.

    abstract::Insulin-like growth factor 2 (IGF2) is an imprinted gene expressed in most tissues affecting lean muscle content in mice, pigs and cattle. We previously identified the bovine IGF2 c.-292C>T SNP in the non-translated exon 2. Using this SNP, we demonstrated biallelic expression of IGF2 after birth. Seven alternatively s...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2007.01576.x

    authors: Goodall JJ,Schmutz SM

    更新日期:2007-04-01 00:00:00

  • Mapping of 443 porcine EST improves the comparative maps for SSC1 and SSC7 with the human genome.

    abstract::Numerous mapping studies of complex traits in the pig have resulted in quantitative trait loci (QTL) intervals of 10-20 cM. To improve the chances to identify the genes located in such intervals, increased expressed sequence tags (EST)-based marker density, coupled with comparative mapping with species whose genomes h...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2005.01328.x

    authors: Demeure O,Pomp D,Milan D,Rothschild MF,Tuggle CK

    更新日期:2005-10-01 00:00:00

  • Expression levels of 25 genes in liver and testis located in a QTL region for androstenone on SSC7q1.2.

    abstract::A quantitative trait locus (QTL) for boar fat androstenone levels has been identified near the SSC7 centromere in a Large White × Meishan cross. Backcrosses were produced to isolate the Chinese haplotype in a European genetic background. The expression of 25 genes from the QTL region was studied in the testes and live...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2011.02195.x

    authors: Robic A,Fève K,Larzul C,Billon Y,van Son M,Liaubet L,Sarry J,Milan D,Grindflek E,Bidanel JP,Riquet J

    更新日期:2011-12-01 00:00:00

  • Characterization of the porcine acyl-CoA synthetase long-chain 4 gene and its association with growth and meat quality traits.

    abstract::Summary Long-chain acyl-CoA synthetase (ACSL) catalyses the formation of long-chain acyl-CoA from fatty acid, ATP and CoA, activating fatty acids for subsequent reactions. Long-chain acyl-CoA synthetase thus plays an essential role in both lipid biosynthesis and fatty acid degradation. The ACSL4 gene was evaluated as ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2006.01436.x

    authors: Mercadé A,Estellé J,Pérez-Enciso M,Varona L,Silió L,Noguera JL,Sánchez A,Folch JM

    更新日期:2006-06-01 00:00:00

  • Detecting selection signatures in three Iranian sheep breeds.

    abstract::The objective of genome mapping is to achieve valuable insight into the connection between gene variants (genotype) and observed traits (phenotype). Part of that objective is to understand the selective forces that have operated on a population. Finding links between genotype-phenotype changes makes it possible to ide...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12772

    authors: Manzari Z,Mehrabani-Yeganeh H,Nejati-Javaremi A,Moradi MH,Gholizadeh M

    更新日期:2019-06-01 00:00:00

  • The genetic structure of Spanish Celtic horse breeds inferred from microsatellite data.

    abstract::Partition of the genetic variability, genetic structure and relationships among seven Spanish Celtic horse breeds were studied using PCR amplification of 13 microsatellites on 481 random individuals. In addition, 60 thoroughbred horses were included. The average observed heterozygosity and the mean number of alleles w...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1046/j.1365-2052.2000.00591.x

    authors: Cañon J,Checa ML,Carleos C,Vega-Pla JL,Vallejo M,Dunner S

    更新日期:2000-02-01 00:00:00

  • Disclosing the origin and diversity of Omani cattle.

    abstract::Among all livestock species, cattle have a prominent status as they have contributed greatly to the economy, nutrition and culture from the beginning of farming societies until the present time. The origins and diversity of local cattle breeds have been widely assessed. However, there are still some regions for which ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2012.02399.x

    authors: Mahgoub O,Babiker HA,Kadim IT,Al-Kindi M,Hassan S,Al-Marzooqi W,Eltahir YE,Al-Abri MA,Al-Khayat A,Al-Sinani KR,Hilal Al-Khanjari H,Costa V,Chen S,Beja-Pereira A

    更新日期:2013-06-01 00:00:00

  • Isolation and sequencing of porcine lipoprotein lipase cDNA and its use in multiallelic restriction fragment length polymorphism detection.

    abstract::Porcine lipoprotein lipase (LPL) cDNA has been cloned and sequenced. The deduced amino acid sequence shows a high degree of identity to LPL from other species, and contains the Ser/His/Asp triade characteristic of serine proteases and esterases. A repetitive element is present in the 3'-untranslated region of the cDNA...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1992.tb00170.x

    authors: Harbitz I,Kristensen T,Kran S,Davies W

    更新日期:1992-01-01 00:00:00

  • Genetic diversity and differentiation of Chinese domestic buffalo based on 30 microsatellite markers.

    abstract::To determine genetic diversity and evolutionary relationships among Chinese buffalo populations, 18 indigenous swamp buffalo populations and two introduced river buffalo breeds were genotyped for 30 microsatellite loci. The mean number of alleles across the 30 loci was 8.13, and the expected heterozygosity ranged from...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2007.01648.x

    authors: Zhang Y,Sun D,Yu Y,Zhang Y

    更新日期:2007-12-01 00:00:00

  • Identifying genetic loci controlling neonatal passive transfer of immunity using a hybrid genotyping strategy.

    abstract::Colostrum intake is critical to a piglet's survival and can be measured by precipitating out the γ-immunoglobulins from serum with ammonium sulfate (immunocrit). Genetic analysis of immunocrits on 5312 piglets indicated that the heritabilities (se) for direct and maternal effects were 0.13 (0.06) and 0.53 (0.08) respe...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12131

    authors: Rohrer GA,Rempel LA,Miles JR,Keele JW,Wiedmann RT,Vallet JL

    更新日期:2014-06-01 00:00:00

  • Biochemical genetic variants in mice selectively bred for sensitivity or resistance to ethanol-induced sedation.

    abstract::The distribution of biochemical genetic variants was examined among eight inbred strains of mice, which served as contributors to a heterogeneous stock of mice (HS), and in short-sleep (SS) and long-sleep (LS) mice, selectively bred from the HS stock for differential ethanol sensitivity. Fifteen loci for enzymes of al...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1986.tb03195.x

    authors: Holmes RS,Petersen DR,Deitrich RA

    更新日期:1986-01-01 00:00:00

  • Polymorphisms in eggshell organic matrix genes are associated with eggshell quality measurements in pedigree Rhode Island Red hens.

    abstract::Novel and traditional eggshell quality measurements were made from up to 2000 commercial pedigree hens for a candidate gene association analysis with organic eggshell matrix genes: ovocleidin-116, osteopontin (SPP1), ovocalyxin-32 (RARRES1), ovotransferrin (LTF), ovalbumin and ovocalyxin-36, as well as key genes in th...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2008.01794.x

    authors: Dunn IC,Joseph NT,Bain M,Edmond A,Wilson PW,Milona P,Nys Y,Gautron J,Schmutz M,Preisinger R,Waddington D

    更新日期:2009-02-01 00:00:00

  • Associations of GH gene variants with performance traits in F2 generations of European wild boar, Piétrain and Meishan pigs.

    abstract::The role of the porcine GH gene was investigated in 292 F2 animals of mating Wild Boar x Piétrain and in 310 F2 animals of mating Meishan x Piétrain. Forty-three traits of fattening, carcass composition, meat quality and stress resistance were recorded. For the analysis of associations between GH gene variants and qua...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.1997.00093.x

    authors: Knorr C,Moser G,Müller E,Geldermann H

    更新日期:1997-04-01 00:00:00

  • The Burmese cat as a genetic model of type 2 diabetes in humans.

    abstract::The recent extension of genetic tools to the domestic cat, together with the serendipitous consequences of selective breeding, have been essential to the study of the genetic diseases that affect them. Cats are increasingly presented for veterinary surveillance and share many of human's heritable diseases, allowing th...

    journal_title:Animal genetics

    pub_type: 杂志文章,评审

    doi:10.1111/age.12799

    authors: Samaha G,Beatty J,Wade CM,Haase B

    更新日期:2019-08-01 00:00:00

  • An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R-banding.

    abstract::Presented herein is an updated sheep cytogenetic map that contains 452 loci (291 type I and 161 type II) assigned to specific chromosome bands or regions on standard R-banded ideograms. This map, which significantly extends our knowledge of the physical organization of the ovine genome, includes new assignments for 88...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2007.01598.x

    authors: Di Meo GP,Perucatti A,Floriot S,Hayes H,Schibler L,Rullo R,Incarnato D,Ferretti L,Cockett N,Cribiu E,Williams JL,Eggen A,Iannuzzi L

    更新日期:2007-06-01 00:00:00

  • Non-synonymous SNPs in MC1R gene are associated with the extended black variant in domestic ducks (Anas platyrhynchos).

    abstract::In this study, we performed a sequence characterization of the duck melanocortin 1 receptor (alpha-melanocyte stimulating hormone receptor) (MC1R) gene to analyze the relationship between MC1R polymorphism and the extended black variant in domestic ducks based on the extended black (E) and non-extended black (e(+) ) a...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2012.02377.x

    authors: Yu W,Wang C,Xin Q,Li S,Feng Y,Peng X,Gong Y

    更新日期:2013-04-01 00:00:00

  • The influence of extracellular matrix and prolactin on global gene expression profiles of primary bovine mammary epithelial cells in vitro.

    abstract::An in vitro bovine mammosphere model was characterized for use in lactational biology studies using a functional genomics approach. Primary bovine mammary epithelial cells cultured on a basement membrane, Matrigel, formed three-dimensional alveoli-like structures or mammospheres. Gene expression profiling during mammo...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2009.01964.x

    authors: Riley LG,Gardiner-Garden M,Thomson PC,Wynn PC,Williamson P,Raadsma HW,Sheehy PA

    更新日期:2010-02-01 00:00:00

  • Mutation in the melanocortin 1 receptor is associated with amber colour in the Norwegian Forest Cat.

    abstract::Amber (previously called X-Colour) is a yellow recessive coat colour observed in the Norwegian Forest Cat (NFC) population and apparently absent in other cat breeds. Until now, there has never been any scientific evidence of yellow recessive mutation (e) reported in the extension gene in Felidae. We sequenced the comp...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2009.01864.x

    authors: Peterschmitt M,Grain F,Arnaud B,Deléage G,Lambert V

    更新日期:2009-08-01 00:00:00

  • Molecular characterization of the human ABO blood group orthologus system in pigs.

    abstract::The selection and use of animals with blood group 0 in the process of transplanting pig organs or tissues into humans can positively contribute to the control of acute immune rejection due to differences in blood groups. Exon-specific PCRs for the porcine blood group A transferase gene against genomic DNA from either ...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2010.02152.x

    authors: Nguyen DT,Choi H,Jo H,Kim JH,Dirisala VR,Lee KT,Kim TH,Park KK,Seo K,Park C

    更新日期:2011-06-01 00:00:00

  • Identification and SNP association analysis of a novel gene in chicken.

    abstract::A novel gene that was predicted to encode a long noncoding RNA (lncRNA) transcript was identified in a previous study that aimed to detect candidate genes related to growth rate differences between Chinese local breed Gushi chickens and Anka broilers. To characterise the biological function of the lncRNA, we cloned an...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/age.12387

    authors: Mei X,Kang X,Liu X,Jia L,Li H,Li Z,Jiang R

    更新日期:2016-02-01 00:00:00

  • Novel alternative splicing by exon skipping in KIT associated with whole-body roan in an intercrossed population of Landrace and Korean Native pigs.

    abstract::The KIT locus has been suggested to be a strong candidate region linked with whole-body roan in the F(2) population produced by intercrosses between Landrace and Korean Native pigs. In this manuscript, we report the finding of a novel alternative splicing event in the porcine KIT gene that results in the skipping of e...

    journal_title:Animal genetics

    pub_type: 杂志文章

    doi:10.1111/j.1365-2052.2011.02225.x

    authors: Lim HT,Zhong T,Cho IC,Seo BY,Kim JH,Lee SS,Ko MS,Park HB,Kim BW,Lee JH,Jeon JT

    更新日期:2011-08-01 00:00:00