Abstract:
:We have for the first time assessed the ability of the Sleeping Beauty (SB) transposon system to enhance transgenesis in chicken and turkey cells. The efficiency of transgenesis with a transposon encoding an antibiotic resistance gene was dramatically enhanced 15- to 35-fold when transposase was supplied by co-transfection of immortalized chicken and turkey cells with a construct encoding SB. In contrast, transgenesis of primary chicken embryo fibroblast (CEF) cells was not significantly increased by providing transposase, suggesting that the benefits of transposon-transgenesis in primary avian cells will require the application of more efficient transfection methods, further enhanced SB transposase or an alternative transposon system.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Kong BW,Carlson DF,Fahrenkrug SC,Foster DNdoi
10.1111/j.1365-2052.2008.01702.xsubject
Has Abstractpub_date
2008-04-01 00:00:00pages
180-6issue
2eissn
0268-9146issn
1365-2052pii
AGE1702journal_volume
39pub_type
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