Application of the Sleeping Beauty transposon system to avian cells.

Abstract:

:We have for the first time assessed the ability of the Sleeping Beauty (SB) transposon system to enhance transgenesis in chicken and turkey cells. The efficiency of transgenesis with a transposon encoding an antibiotic resistance gene was dramatically enhanced 15- to 35-fold when transposase was supplied by co-transfection of immortalized chicken and turkey cells with a construct encoding SB. In contrast, transgenesis of primary chicken embryo fibroblast (CEF) cells was not significantly increased by providing transposase, suggesting that the benefits of transposon-transgenesis in primary avian cells will require the application of more efficient transfection methods, further enhanced SB transposase or an alternative transposon system.

journal_name

Anim Genet

journal_title

Animal genetics

authors

Kong BW,Carlson DF,Fahrenkrug SC,Foster DN

doi

10.1111/j.1365-2052.2008.01702.x

subject

Has Abstract

pub_date

2008-04-01 00:00:00

pages

180-6

issue

2

eissn

0268-9146

issn

1365-2052

pii

AGE1702

journal_volume

39

pub_type

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