Abstract:
:Genomic imprinting is a mammalian developmental process that uses epigenetic mechanisms to induce monoallelic and parental-specific expression of particular autosomal genes. A crucial epigenetic event consists of DNA methylation of CpG-islands, which become differentially methylated regions (DMRs) on the maternal and paternal alleles during oogenesis or spermatogenesis (germline DMRs). By contrast, somatic DMRs are acquired after fertilization. While there are several studies referring to methylation acquisition within germline DMRs in the mouse and human, a comparable methylation analysis of orthologous sequences is still lacking in sheep. To identify germline DMRs, this study analysed the methylation status of the available CpG-islands of five ovine imprinted genes (H19, IGF2R, DLK1, DIO3 and BEGAIN) in mature spermatozoa and in female gametes at different stages of their follicle growth, including in vitro matured oocytes. The 5'-end CpG-island of H19 showed a full methylation in spermatozoa and an absent methylation in growing and fully grown oocytes. The intron 2 CpG-island of IGF2R was unmethylated in male gametes, while it showed a high level of methylation in early stages of oogenesis. The promoter CpG-islands of DLK1 and DIO3 were found to be unmethylated both in spermatozoa and oocytes. Finally, the exon 9 CpG-island of BEGAIN was hypermethylated in mature male gametes, while it showed an almost complete methylation only in late stages of oocyte development. Our findings suggest that DNA methylation establishment during early stages of sheep oogenesis and subsequent in vitro maturation is gene-specific and that, of the five genes investigated, only the CpG-islands of H19 and IGF2R might represent ovine germline DMRs.
journal_name
Anim Genetjournal_title
Animal geneticsauthors
Colosimo A,Di Rocco G,Curini V,Russo V,Capacchietti G,Berardinelli P,Mattioli M,Barboni Bdoi
10.1111/j.1365-2052.2009.01939.xsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
900-8issue
6eissn
0268-9146issn
1365-2052pii
AGE1939journal_volume
40pub_type
杂志文章相关文献
ANIMAL GENETICS文献大全abstract::The C3 polymorphism of equine serum or plasma revealed by agarose gel electrophoresis can be diagnosed with protein stain following acid protein fixation. In addition to the three alleles previously described (C31, C32, C33), a fourth allele (C34) was found. Population data for 25 domestic breeds and Equus przewalskii...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1990.tb03210.x
更新日期:1990-01-01 00:00:00
abstract::Single nucleotide polymorphisms (SNPs) in the calpain 1 (CAPN1) and calpastatin (CAST) genes were studied to determine their effects on meat tenderness in Bos taurus cattle. Strip loins (M. longissimus dorsi) were removed from cattle in four resource populations after slaughter (n = 1042), aged under controlled condit...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2006.01483.x
更新日期:2006-08-01 00:00:00
abstract::The stearoyl-CoA desaturase (delta-9-desaturase; SCD) gene is a candidate gene for fatty acid composition. It is located on pig SSC14 in a region where quantitative trait loci (QTL) for fatty acid composition were previously detected in a Duroc purebred population. The objective of the present study was to fine map th...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2011.02236.x
更新日期:2012-04-01 00:00:00
abstract::We determined the genetic diversity and evolutionary relationships among 26 Chinese indigenous horse breeds and two introduced horse breeds by genotyping these animals for 27 microsatellite loci. The 26 Chinese horse breeds come from 12 different provinces. Two introduced horse breeds were the Mongolia B Horse from Mo...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2010.02067.x
更新日期:2011-02-01 00:00:00
abstract::A canine blood group antigen, QN, which was detected by a naturally occurring alloantibody in the antiglobulin test, was shown to be antigenically related to the human A and cattle J antigens by absorption experiments. Family studies supported a dominant mode of inheritance with the gene controlling the production of ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1992.tb00153.x
更新日期:1992-01-01 00:00:00
abstract::The genetic consequences of population differentiation and isolation have been the subject of conservation biology. In this study, we analysed the genetic diversity and structure of Mongolian sheep in China. These animals belong to a traditional local breed with high production, extensive adaption, early maturity and ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2011.02181.x
更新日期:2011-10-01 00:00:00
abstract::A new variant of glucose phosphate isomerase (GPI), also known as phosphohexose isomerase (PHI), was detected in a primitive pig population. ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1988.tb00790.x
更新日期:1988-01-01 00:00:00
abstract::The ovine NRAMP1 and cervine NRAMP1 cDNAs were cloned by RT PCR of RNA derived from macrophage enriched leukocyte preparations. The complete coding and 3' regions were sequenced. Both sheep and deer NRAMP1 proteins are 548 amino acids long. There are 77 and 73 amino acid differences, respectively, compared to the mous...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.1998.00224.x
更新日期:1998-02-01 00:00:00
abstract::In domestic goats, the polled intersex syndrome (PIS) refers to XX female-to-male sex reversal associated with the absence of horn growth (polled). The causal variant was previously reported as a 11.7 kb deletion at approximately 129 Mb on chromosome 1 that affects the transcription of both FOXL2 and several long non-...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12918
更新日期:2020-06-01 00:00:00
abstract::We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1992.tb00180.x
更新日期:1992-01-01 00:00:00
abstract::The extent of linkage disequilibrium (LD) between genetic loci has implications for both association studies and the accuracy of genomic prediction. To characterise the persistence of LD in diverse sheep breeds, two SNP genotyping platforms were used. First, existing SNP genotypes from 63 breeds obtained using the ovi...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12197
更新日期:2014-10-01 00:00:00
abstract::Supernumerary teats represent a common abnormality of the bovine udder. A genome-wide association study was performed based on the proportion of the occurrence of supernumerary teats in the daughters of 1097 Holstein bulls. The heritability of caudal supernumerary teats without mammary gland in this study was 0.604. T...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12215
更新日期:2014-12-01 00:00:00
abstract::In the present study, 25 195 BAC ends for channel catfish (Ictalurus punctatus) were sequenced, generating 20 366 clean BAC-end sequences (BES), with an average read length of 557 bp after trimming. A total of 11 414 601 bp were generated, representing approximately 1.2% of the catfish genome. Based on this survey, th...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2006.01453.x
更新日期:2006-08-01 00:00:00
abstract::The genetic differentiation and phylogenetic relationships of 18 indigenous goat populations from seven East Asian countries were analysed based on data obtained from 26 microsatellite DNA markers. The mean number of alleles (MNA) per population ranged from 2.5 to 7.6, with an average of 5.8. Genetic variability estim...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2012.02334.x
更新日期:2012-12-01 00:00:00
abstract::An important aim in animal breeding is the improvement of growth and meat quality traits. Previous studies have demonstrated that genetic variants in the fat mass and obesity associated (FTO) gene have a relatively large effect on human obesity as well as on body composition in rodents and, more recently, in livestock...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12403
更新日期:2016-04-01 00:00:00
abstract::The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene. A single positive recombinant phage clone containing a 10.7-kb insert was isolated from a porcine genomic library, and a 4.2-kb fragment was subcloned and sequenced. The 4.2-kb fragment contained the e...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1046/j.1365-2052.1998.00273.x
更新日期:1998-02-01 00:00:00
abstract::A quantitative trait locus (QTL) for boar fat androstenone levels has been identified near the SSC7 centromere in a Large White × Meishan cross. Backcrosses were produced to isolate the Chinese haplotype in a European genetic background. The expression of 25 genes from the QTL region was studied in the testes and live...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2011.02195.x
更新日期:2011-12-01 00:00:00
abstract::In order to obtain monoclonal alloantibodies against bovine blood group antigens, lymph node cells from calves immunized with bovine red blood cells (RBC) were fused with either murine NSO/1 myeloma cells or a HAT sensitive murine x bovine heterohybridoma cell line. Both fusion partners resulted in heterohybridoma cel...
journal_title:Animal genetics
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::Overlapping runs of homozygosity (ROH islands) shared by the majority of a population are hypothesized to be the result of selection around a target locus. In this study we investigated the impact of selection for coat color within the Noriker horse on autozygosity and ROH patterns. We analyzed overlapping homozygous ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12797
更新日期:2019-08-01 00:00:00
abstract::Two new alleles (A and E) of the bovine MUC locus which encodes PAS-1 protein, a glycoprotein of the milk fat globule membrane, are reported. The A allele was found in Italian Brown while E was present in the Jersey and the Piedmont breeds. ...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1995.tb03163.x
更新日期:1995-06-01 00:00:00
abstract::Ovine hereditary chondrodysplasia, or spider lamb syndrome (SLS), is a genetic disorder that is characterized by severe skeletal abnormalities and has resulted in substantial economic losses for sheep producers. Here we demonstrate that a non-synonymous T>A transversion in the highly conserved tyrosine kinase II domai...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2005.01398.x
更新日期:2006-02-01 00:00:00
abstract::RNA editing is a post-transcription maturation process that diversifies genomically encoded information and can lead to transcriptome diversity. Thanks to next-generation sequencing technologies, a large number of editing sites have been identified in different species. Although this mechanism is well described in mam...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12818
更新日期:2019-10-01 00:00:00
abstract::We have previously identified and mapped porcine expressed sequence tags (ESTs) derived from genes that are preferentially expressed in liver. The aim of the present study was to identify single nucleotide polymorphisms (SNPs) in porcine genes encoding enzymes in hepatic metabolic pathways and use the SNPs for mapping...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2005.01351.x
更新日期:2005-12-01 00:00:00
abstract::Dexter cattle carry a genetic defect causing a dwarf phenotype in the heterozygotes (Dx+/-), while homozygotes (Dx+/+) are stillborn with extreme shortening of limbs and gross craniofacial defects and are described as 'bulldog' calves. The heterozygous phenotype has been likened to achondroplastic dwarfism in humans (...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1997.00068.x
更新日期:1997-02-01 00:00:00
abstract::Quantitative trait loci (QTL) mapping for susceptibility to a Salmonella Abortusovis vaccinal strain was performed using an experimental design involving 30 Romane sheep sire families (1216 progenies). Nine QTL corresponding to bacterial load, weight variations and antibody response criteria were mapped on eight chrom...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.2011.02291.x
更新日期:2012-10-01 00:00:00
abstract::The Ehlers-Danlos syndromes (EDSs) are a heterogeneous group of inherited connective tissue disorders characterized by skin hyperextensibility, joint hypermobility and tissue fragility. Inherited disorders similar to human EDS have been reported in different mammalian species. In the present study, we investigated a f...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12830
更新日期:2019-10-01 00:00:00
abstract::Two new haemoglobin variants, provisionally named Hb G and Hb H, were found during a survey of 295 Welsh Mountain cross-bred sheep. Both haemoglobins appear to be beta chain variants controlled by genes allelic to those for the common forms Hb A and Hb B. Studies on an anaemic Hb AH and an Hb AG type sheep showed that...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/j.1365-2052.1990.tb03215.x
更新日期:1990-01-01 00:00:00
abstract::The optimal management of the commercially important, but mostly over-exploited, pelagic tunas, albacore (Thunnus alalunga Bonn., 1788) and Atlantic bluefin tuna (BFT; Thunnus thynnus L., 1758), requires a better understanding of population structure than has been provided by previous molecular methods. Despite numero...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12051
更新日期:2013-12-01 00:00:00
abstract::Recently, the Swiss breeding association reported an increasing number of white-spotted cattle in the Brown Swiss breed, which is normally solid brown coloured. A total of 60 Brown Swiss cattle with variably sized white abdominal spots, facial markings and depigmented claws were collected for this study. A genome-wide...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12751
更新日期:2019-02-01 00:00:00
abstract::The bumblebee, Bombus terrestris, is an important pollinator commercially used on a global scale. The exported subspecies B. t. terrestris has colonised diverse environments, in some cases displacing wild pollinators to the verge of local extinction. In this sense, the native Iberian subspecies B. t. lusitanicus may b...
journal_title:Animal genetics
pub_type: 杂志文章
doi:10.1111/age.12874
更新日期:2020-02-01 00:00:00