Abstract:
:The relationship between oxidative stress and miRNA changes in placenta as a potential mechanism involved in preeclampsia (PE) is not fully elucidated. We investigated the impact of oxidative stress on miRNAs and mRNA expression profiles of genes associated with PE in villous 3A first trimester trophoblast cells exposed to H2O2 at 12 different concentrations (0-1 mM) for 0.5, 4, 24, and 48 h. Cytotoxicity, determined using the SRB assay, was used to calculate the IC50 of H2O2. RNA was extracted after 4 h exposure to H2O2 for miRNA and gene expression profiling. H2O2 exerted a concentration- and time-dependent cytotoxicity on 3A trophoblast cells. Short-term exposure of 3A cells to low concentration of H2O2 (5% of IC50) significantly altered miRNA profile as evidenced by significant changes in 195 out of 595 evaluable miRNAs. Tool for annotations of microRNAs (TAM) analysis indicated that these altered miRNAs fall into 43 clusters and 34 families, with 41 functions identified. Exposure to H2O2 altered mRNA expression of 22 out of 84 key genes involved in dysregulation of placental development. In conclusion, short-term exposure of villous first trimester trophoblasts to low concentrations of H2O2 significantly alters miRNA profile and expression of genes implicated in placental development.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Cross CE,Tolba MF,Rondelli CM,Xu M,Abdel-Rahman SZdoi
10.1155/2015/257090subject
Has Abstractpub_date
2015-01-01 00:00:00pages
257090eissn
2314-6133issn
2314-6141journal_volume
2015pub_type
杂志文章abstract:OBJECTIVE:Fever in systemic lupus erythematosus (SLE) can be caused by infection or flare-up of the disease. This study aimed to determine whether the ratio of the level of erythrocyte-bound C4d to that of complement receptor 1 (C4d/CR1) can serve as a useful biomarker in the differentiation between infection and flare...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/939783
更新日期:2015-01-01 00:00:00
abstract:Background:Comparison of single-level open and minimally invasive transforaminal lumbar interbody fusions (O-TLIF and MI-TLIF) of a single surgeon and presentation of his MI-TLIF learning curve in a retrospective observational cohort study. Methods:27 MI-TLIF and 31 O-TLIF patients, performed between 03/01/2013 and 03...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/3798537
更新日期:2020-01-25 00:00:00
abstract::Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic aci...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/391070
更新日期:2015-01-01 00:00:00
abstract::Human dental pulp stem cells (DPSCs) hold great promise in bone regeneration. However, the exact mechanism of osteogenic differentiation of DPSCs remains unknown, especially the role of exosomes played in. The DPSCs were cultured and received osteogenic induction; then, exosomes from osteogenic-induced DPSCs (OI-DPSC-...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6319395
更新日期:2020-09-28 00:00:00
abstract:Objective:To investigate the clinicopathological characteristics and risk factors in patients with lung cancer and COPD. Materials and Methods:We retrospectively reviewed the clinical data of 282 patients with lung cancer, including 174 and 108 patients with and without COPD, respectively. Information on age, sex, smo...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8398156
更新日期:2018-02-14 00:00:00
abstract::The 13 million nurses worldwide constitute most of the global healthcare workforce and are uniquely positioned to engage with others to address disparities in healthcare to achieve the goal of better health for all. A new vision for nurses involves active participation and collaboration with international colleagues a...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2016/3127543
更新日期:2016-01-01 00:00:00
abstract::The current data are still inconclusive in terms of a genetic component involved in the susceptibility to renal cell carcinoma. Our aim was to evaluate 40 selected candidate polymorphisms for potential association with clear cell renal cell carcinoma (ccRCC) based on independent group of 167 patients and 200 healthy c...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2015/860405
更新日期:2015-01-01 00:00:00
abstract::This study aimed at clarifying the mechanism and role of survivin in hypoxia-induced multidrug resistance (MDR) of laryngeal carcinoma cells. Human laryngeal cancer cells were incubated under hypoxia or normoxia. The expression of survivin was silenced by performing RNA interference. Additionally, by Western blot and ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/5696801
更新日期:2019-04-24 00:00:00
abstract::Dental modeling resins have been developed for use in areas where highly precise resin structures are needed. The manufacturers claim that these polymethyl methacrylate/methyl methacrylate (PMMA/MMA) resins show little or no shrinkage after polymerization. This study examined the polymerization shrinkage of five denta...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/914739
更新日期:2014-01-01 00:00:00
abstract:PURPOSE:To determine the predictive factors that affect short term and long term postoperative drift in intermittent exotropia after bilateral lateral rectus recession and to evaluate its effect on surgical outcome. METHODS:Retrospective review of 203 patients with diagnosis of intermittent exotropia, who had surgical...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/482093
更新日期:2014-01-01 00:00:00
abstract::Mycobacterium bovis causes classic bovine tuberculosis, a zoonosis which is still a concern in Africa. Biofilm forming ability of two Mycobacterium bovis strains was assessed on coupons of cement, ceramic, or stainless steel in three different microbiological media at 37°C with agitation for 2, 3, or 4 weeks to determ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/210165
更新日期:2014-01-01 00:00:00
abstract:Objective:Biomaterial research for soft tissue augmentation is an increasing topic in aesthetic medicine. Hyaluronic acid (HA) fillers are widely used for their low invasiveness and easy application to correct aesthetic defects or traumatic injuries. Some complications as acute or chronic inflammation can occur in pati...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8676343
更新日期:2020-05-21 00:00:00
abstract::The perceived risk of nonremunerated blood donation (NRBD) is one of the most important factors which hinder the Chinese public from donating blood. To understand deeply and measure scientifically the public's perceived risk of NRBD, in this paper the qualitative and quantitative methods were used to explore the const...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/302043
更新日期:2015-01-01 00:00:00
abstract::Caudal epidural block is a commonly used technique for surgical anesthesia in children and chronic pain management in adults. It is performed by inserting a needle through the sacral hiatus to gain entrance into the sacral epidural space. Using conventional blind technique, the failure rate of caudal epidural block in...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/9217145
更新日期:2017-01-01 00:00:00
abstract::Breast cancer is the most frequently occurring disease among women worldwide. The early stage of breast cancer identification is the key challenge in cancer control and prevention procedures. Although gene expression profiling helps to understand the molecular mechanism of diseases or disorder in the living system, ge...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3071013
更新日期:2016-01-01 00:00:00
abstract::Alzheimer's disease (AD) and multiple sclerosis are major neurodegenerative diseases, which are characterized by the accumulation of abnormal pathogenic proteins due to oxidative stress, mitochondrial dysfunction, impaired autophagy, and pathogens, leading to neurodegeneration and behavioral deficits. Herein, we revie...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2018/1483791
更新日期:2018-07-12 00:00:00
abstract::In continuance of our search for anticancer agents, we report herein the synthesis and anticancer activity of some novel oxadiazole analogues. The compounds were screened for anticancer activity as per National Cancer Institute (NCI US) protocol on leukemia, melanoma, lung, colon, CNS, ovarian, renal, prostate, and br...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/814984
更新日期:2014-01-01 00:00:00
abstract::Cabbages (Brassica oleracea L.) are an important vegetable crop around world, and cold temperature is among the most significant abiotic stresses causing agricultural losses, especially in cabbage crops. Plant bZIP transcription factors play diverse roles in biotic/abiotic stress responses. In this study, 119 putative...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/4376598
更新日期:2016-01-01 00:00:00
abstract:INTRODUCTION:This paper presents the final results of a cross-sectional study started in 2010. It compares the perceived efficacy of different types of tobacco health warning (texts versus shocking pictures) to quit or reduce tobacco use. METHODS:The study conducted between 2010 and 2012 in Italy enrolled adults smoke...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/723035
更新日期:2014-01-01 00:00:00
abstract::To study the effect of intra-abdominal hypertension (IAH) on the frequency of pneumonia with an experimental study, thirteen Sprague-Dawley rats were included. Eight out of thirteen animals were randomly assigned to receive 10 ml of benzalkonium chloride 0.2% (megacolon group) and five animals received 10 ml NaCl 0.9%...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/4601348
更新日期:2017-01-01 00:00:00
abstract::Mammalian protection against leishmanial infection depends on the development of an effective immune response. Zoonotic visceral leishmaniasis (ZVL) patients are usually unable to mount an effective immune response against the parasite and indeed appear to be severely immunosuppressed. This suppression has strong nons...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/5861453
更新日期:2017-01-01 00:00:00
abstract::Surgical flaps are frequently affected by ischemia/reperfusion (I/R) injury. Calcium-sensing receptor (CaSR) and stromal cell-derived factor-1α (SDF-1α) are closely associated with myocardial I/R injury. This study was performed to evaluate the feasibility of applying SDF-1α to counteract CaSR activation-mediated I/R ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8945850
更新日期:2018-01-11 00:00:00
abstract::[This corrects the article DOI: 10.1155/2016/6935123.]. ...
journal_title:BioMed research international
pub_type: 已发布勘误
doi:10.1155/2017/1641589
更新日期:2017-01-01 00:00:00
abstract::Exclusive enteral nutrition (EEN) has been proven to be effective and safe in treating pediatric Crohn's disease (CD). EEN induces pediatric CD remission possibly through three pathways: (1) direct anti-inflammatory effects, (2) improved epithelial barrier function, and (3) modulation of the gut microbiota. Recent stu...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/8102589
更新日期:2017-01-01 00:00:00
abstract::Little is known about how small variations in ionic currents and Ca²⁺ and Na⁺ diffusion coefficients impact action potential and Ca²⁺ dynamics in rabbit ventricular myocytes. We applied sensitivity analysis to quantify the sensitivity of Shannon et al. model (Biophys. J., 2004) to 5%-10% changes in currents conductanc...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/565431
更新日期:2013-01-01 00:00:00
abstract::Toll-like receptors (TLRs) play a key role in the innate immune response to numerous pathogens, including Acanthamoeba spp. The aim of this study was to determine the expression of TLR2 and TLR4 in the eyes of mice following intranasal infection with Acanthamoeba spp. in relation to the host's immunological status. Am...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/1401894
更新日期:2019-06-12 00:00:00
abstract:BACKGROUND:Inflammation has a key role in migraine pathophysiology. Vitamin D is an effective anti-inflammatory agent. The aim of this study was to investigate the association between migraine and two vitamin D receptor (VDR) polymorphisms (TaqI and FokI) and also the relationship between VDR polymorphisms and headache...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/351942
更新日期:2013-01-01 00:00:00
abstract::Spinal cord injured (SCI) individuals show an altered hemodynamic response to metaboreflex activation due to a reduced capacity to vasoconstrict the venous and arterial vessels below the level of the lesion. Exercise training was found to enhance circulating catecholamines and to improve cardiac preload and venous ton...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/893468
更新日期:2014-01-01 00:00:00
abstract:Aim:Little is known about the association between cancer antigen 125 (MUC16/CA125) concentrations and tumor diameter of patients with hepatocellular carcinoma (HCC) and low AFP levels. To fill this gap in our knowledge, we conducted a retrospective study of 427 patients with HCC with AFP ≤200 ng/mL who underwent R0 res...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6959637
更新日期:2019-09-29 00:00:00
abstract:PURPOSE:Suppressor of cytokine signaling 7 (SOCS7) is a member of the SOCS family and is known to interact with phospholipase Cγ-1 (PLCγ-1), a key downstream mediator of the hepatocyte growth factor (HGF)/C-MET axis. Here, we report our observations of the effect of knocking down SOCS7 gene on the behaviour of breast c...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/648040
更新日期:2014-01-01 00:00:00