A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms.

Abstract:

:Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.

journal_name

Biomed Res Int

authors

Zhang J,Wu H,Chen Q,Zhao P,Zhao H,Yao W,Wei M

doi

10.1155/2015/391070

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

391070

eissn

2314-6133

issn

2314-6141

journal_volume

2015

pub_type

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