Abstract:
:Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1 C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Zhang J,Wu H,Chen Q,Zhao P,Zhao H,Yao W,Wei Mdoi
10.1155/2015/391070subject
Has Abstractpub_date
2015-01-01 00:00:00pages
391070eissn
2314-6133issn
2314-6141journal_volume
2015pub_type
杂志文章abstract::Cancer cells acquire unique secretome compositions that contribute to tumor development and metastasis. The aim of our study was to elucidate the biological processes involved in cervical cancer, by performing a proteomic analysis of the secretome from the following informative cervical cell lines: SiHa (HPV16+), HeLa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/4180703
更新日期:2017-01-01 00:00:00
abstract::Interest is rising for animal modeling of pathological gambling. Using the operant probabilistic-delivery task (PDT), gambling proneness can be evaluated in laboratory animals. Drawing a comparison with rats, this study evaluated the common marmoset (Callithrix jacchus) using a PDT. By nose- or hand-poking, subjects l...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/927685
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To assess quality of care of women with severe maternal morbidity and to identify associated factors. METHOD:This is a national multicenter cross-sectional study performing surveillance for severe maternal morbidity, using the World Health Organization criteria. The expected number of maternal deaths was cal...
journal_title:BioMed research international
pub_type: 杂志文章,多中心研究
doi:10.1155/2014/989815
更新日期:2014-01-01 00:00:00
abstract::Oyster mushroom Pleurotus florida was cultivated on different combinations of wheat straw (WS) as basal substrate and oyster shell powder (OSP) supplement. The OSP supplementation considerably responded to different cultivation phases. The mycelium grew fast and showed rapid growth rate (8.91 mmd(-1)) in WS + OSP (97 ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/386265
更新日期:2014-01-01 00:00:00
abstract::Cystic change in metastatic lymph nodes of papillary thyroid carcinoma (PTC) is a diagnostic challenge for fine needle aspiration (FNA) because of the scant cellularity. The aim of this study was to evaluate the measurement of thyroglobulin in fine needle aspirate (Tg-FNA) for detecting metastatic PTC in patients with...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/8905916
更新日期:2016-01-01 00:00:00
abstract::[This corrects the article DOI: 10.1155/2017/5760612.]. ...
journal_title:BioMed research international
pub_type: 杂志文章,已发布勘误
doi:10.1155/2018/7108272
更新日期:2018-01-08 00:00:00
abstract::Prostate cancer is a life-threatening and highly heterogeneous malignancy. In the past decade, circulating tumor cells (CTCs) have been suggested to play a critical role in the occurrence and progression of prostate cancer. In particular, as the "seed" of the cancer metastasis cascade, CTCs determine numerous biologic...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2021/6230826
更新日期:2021-01-08 00:00:00
abstract::Cancer is known to have unique metabolic features such as Warburg effect. Current cancer therapy has moved forward from cytotoxic treatment to personalized, targeted therapies, with some that could lead to specific metabolic changes, potentially monitored by imaging methods. In this paper we addressed the important as...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/625095
更新日期:2014-01-01 00:00:00
abstract:Background:A multifactorial decision support system (mDSS) is a tool designed to improve the clinical decision-making process, while using clinical inputs for an individual patient to generate case-specific advice. The study provides an overview of the literature to analyze current available mDSS focused on prostate ca...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2019/4961768
更新日期:2019-06-06 00:00:00
abstract::A dentin-cement-prosthesis complex restored with either all-porcelain, cured resin-composite, or cast base metal alloy and cemented with either of the different resin cements was trimmed into a mini-dumbbell shape for tensile testing. The fractured surfaces and characterization of the dentin-cement interface of bonded...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/656948
更新日期:2015-01-01 00:00:00
abstract::Background. Endemic areas of tegumentary leishmaniasis (TL) in Salta, Argentina, present some overlap zones with the geographical distribution of Chagas disease, with mixed infection cases being often detected. Objectives. The purpose of this study was to determine the magnitude of Leishmania sp. infection and potenti...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/6456031
更新日期:2016-01-01 00:00:00
abstract::Different animal models for peritoneal dialysis (PD) have been used in the past decades to develop PD fluids compatible with patient life and to identify markers of peritoneal fibrosis and inflammation. Only few of those studies have taken into account the importance of uraemia-induced alterations at both systemic and...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/106902
更新日期:2015-01-01 00:00:00
abstract::Prior to the "onset of hearing," developing cochlear inner hair cells (IHCs) and primary auditory neurons undergo experience-independent activity, which is thought to be important in retaining and refining neural connections in the absence of sound. One of the major hypotheses regarding the origin of such activity inv...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/367939
更新日期:2014-01-01 00:00:00
abstract::Integrin αIIbβ3 plays a crucial role in the process of platelet aggregation. Three integrin αIIbβ3 antagonists (abciximab, eptifibatide, and tirofiban) have been approved by FDA for clinical use. Unfortunately, they all showed severe side effects such as thrombocytopenia and bleeding risk. Thus, researches on the deve...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/8587164
更新日期:2016-01-01 00:00:00
abstract::Giardia duodenalis is a small intestinal protozoan parasite of several terrestrial vertebrates. This work aims to assess the genotypic variability of Giardia duodenalis isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (gdh) and ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/875048
更新日期:2013-01-01 00:00:00
abstract::There were no systematic researches about autophagy-related long noncoding RNA (lncRNA) signatures to predict the survival of patients with colon adenocarcinoma. It was necessary to set up corresponding autophagy-related lncRNA signatures. The expression profiles of lncRNAs which contained 480 colon adenocarcinoma sam...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9807918
更新日期:2020-09-02 00:00:00
abstract::Metastasis is the leading cause of lung cancer-associated death. Downregulated expression of E-cadherin followed by epithelial-mesenchymal transition (EMT) is critical for metastasis initiation in lung cancer. BTBD7 plays essential roles in lung cancer metastasis, but the mechanisms remain unknown. This study aimed to...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/5937635
更新日期:2019-11-27 00:00:00
abstract::Mouse lines selected for maximal (AIRmax) or minimal acute inflammatory reaction (AIRmin) were used to characterize the immune response and the influence of genetic background during pristane-induced arthritis (PIA). Susceptible AIRmax mice demonstrated exacerbated cellular profiles during PIA, with intense infiltrati...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1267038
更新日期:2018-10-08 00:00:00
abstract:Objective:It is sometimes difficult to obtain complete/partial response of large hepatocellular carcinoma (HCC) nodules by trans-arterial chemoembolization/embolization (TACE/TAE). The aim is retrospective investigation of tumor response of large HCC nodules (>7 cm) treated by the new TACE technique, repeated alternate...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9289321
更新日期:2020-01-19 00:00:00
abstract:Objective:This study evaluated the bioactivity and physicochemical properties of three commercial calcium silicate-based endodontic materials (MTA, EndoSequence Root Repair Material putty, and Biodentine™). Material and Methods. Horizontal sections of 3 mm thickness from 18 root canals of human teeth were subjected to ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/9576930
更新日期:2020-05-22 00:00:00
abstract::The purpose of this study was to describe the first metabolic profile of human sperm cells through the application of an untargeted platform based on gas chromatography-mass spectrometry (GC-MS). Sperm cell samples from patients diagnosed with idiopathic asthenozoospermia (n = 30) and healthy subjects (n = 30) were an...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8327506
更新日期:2018-02-28 00:00:00
abstract::Cancer and diabetes are among the most common diseases in western societies. Epidemiological studies have shown that diabetic patients have a significantly higher risk of developing a number of different types of cancers and that individuals with comorbidity (cancer and diabetes/prediabetes) have a poorer prognosis re...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/165105
更新日期:2015-01-01 00:00:00
abstract::Objectives. The aim of the study is to detect the prevalence and the characteristics of infraorbital canal and Haller's cells on panoramic radiography of edentulous patients. Methods. The study group comprised 291 panoramic radiographs of edentulous patients. Radiographs were interpreted for the visibility and charact...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1293124
更新日期:2018-07-08 00:00:00
abstract:UNLABELLED:Calcium oxalate nephrolithiasis is the most common urological disease, but noninvasive and convenient methods of diagnosis are rarely available. OBJECTIVE:The present study aimed to identify potential urine biomarkers for noninvasive diagnosis of CaOx nephrolithiasis. METHODOLOGY:Urine samples from 72 pati...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/415651
更新日期:2014-01-01 00:00:00
abstract::The AT-hook transcription factor, AKNA, is a nuclear protein that affects a few physiological and pathological processes including cancer. Here, we investigated the role of AKNA in gastric cancer (GC). By using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot assays, AKNA was found deregulat...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6726759
更新日期:2020-05-13 00:00:00
abstract:PURPOSE:Recovery of walking outdoors after hip fracture is important for equal participation in the community. The causes of poor recovery are not fully understood. This study investigates recovery of walking outdoors and associated determinants after hip fracture. METHODS:A prospective follow-up study, among clinical...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/289549
更新日期:2014-01-01 00:00:00
abstract::The repair of bone defects is a big challenge in reconstructive surgery. Periosteal distraction osteogenesis (PDO), as a promising technique used for bone regeneration, forms a space between the periosteum and bone cortex to regenerate the new bone merely by distracting the periosteum. In order to investigate the infl...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7381391
更新日期:2020-04-23 00:00:00
abstract::Diarrhea is a major clinical problem affecting foals up to 3 months of age. The aim of this study was to identify enteric microorganisms involved in monoinfections and coinfections and the associated virulence factors in healthy and diarrheic foals. Diarrheic (D) (n = 56) and nondiarrheic (ND) foals (n = 60) up to thr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/1512690
更新日期:2016-01-01 00:00:00
abstract::Morphine is considered a highly potent analgesic agent used to relieve suffering of patients with cancer. Several in vitro and in vivo studies showed that morphine also modulates angiogenesis and regulates tumour cell growth. Unfortunately, the results obtained by these studies are still contradictory. In order to bet...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/161508
更新日期:2015-01-01 00:00:00
abstract::Seed inoculation with bacterial consortium was found to increase legume yield, providing a higher growth than the standard nitrogen treatment methods. Alfalfa plants were inoculated by mono- and binary compositions of nitrogen-fixing microorganisms. Their physiological and biochemical properties were estimated. Inocul...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/202597
更新日期:2015-01-01 00:00:00