In vitro and in vivo effects of suppressor of cytokine signalling 7 knockdown in breast cancer: the influence on cellular response to hepatocyte growth factor.

Abstract:

PURPOSE:Suppressor of cytokine signaling 7 (SOCS7) is a member of the SOCS family and is known to interact with phospholipase Cγ-1 (PLCγ-1), a key downstream mediator of the hepatocyte growth factor (HGF)/C-MET axis. Here, we report our observations of the effect of knocking down SOCS7 gene on the behaviour of breast cancer cells both in vitro and in vivo and to elucidate whether this involves HGF/C-MET pathway using the PLCγ-1 blocker U73122. METHODS:MCF7 and MDA-MB-231 breast cancer cells were transfected with anti-SOCS7 ribozymal transgene, to create sublines with SOCS7 knockdown. The in vitro growth and migration of the cells were evaluated in basic conditions and with HGF and U73122 treatment using growth assays, scratch-wound, and electrical cell impedance sensing (ECIS) migration assays. MCF7 and MDA-MB-231 in vivo tumour xenograft growth were also studied. RESULTS:Basal in vitro growth and migration of both cellular lines and the in vivo MCF7 xenograft growth were significantly enhanced with SOCS7 knockdown. In vitro HGF treatment has further influenced the growth and migration when SOCS7 gene was knocked-down in both cellular lines (P < 0.05). PLCγ-1 pharmacological inhibition of the HGF/C-MET cascade during their in vitro growth and migration seemed to only occur when SOCS7 gene was knocked down. CONCLUSIONS:We report a unique regulatory role for SOCS7 in controlling the malignant behaviour of breast cancer lines MCF7 and MDA-MB-231 in vitro and the MCF7 tumour xenografts in vivo. We also report a regulatory role for SOCS7 during the in vitro HGF-induced growth and migration in these cells as HGF treatment and SOCS7 loss have synergistically enhanced these functions. This SOCS7 knockdown-attributed effect could be due to a precise anti-PLCγ-1 role.

journal_name

Biomed Res Int

authors

Sasi W,Ye L,Jiang WG,Sharma AK,Mokbel K

doi

10.1155/2014/648040

subject

Has Abstract

pub_date

2014-01-01 00:00:00

pages

648040

eissn

2314-6133

issn

2314-6141

journal_volume

2014

pub_type

杂志文章
  • Enhanced Production of Polymyxin E in Paenibacillus polymyxa by Replacement of Glucose by Starch.

    abstract::Polymyxin E or colistin, produced by Paenibacillus polymyxa, is an important antibiotic against Gram-negative pathogens. The objective of this study is to evaluate the effect of starch in fermentation medium on colistin biosynthesis in P. polymyxa. The results indicated that replacement of glucose by starch stimulated...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/1934309

    authors: Yu Z,Sun Z,Yin J,Qiu J

    更新日期:2018-10-14 00:00:00

  • Varicocele Repair Improves Testicular Histology in Men with Nonobstructive Azoospermia.

    abstract:OBJECTIVE:To determine the histopathological differences after varicocele repair in testicular tissue in males with nonobstructive azoospermia. METHODS:Between 2009 and 2014, 45 men with complete azoospermia and palpable varicocele, presenting with primary infertility of at least 1 year, undergoing varicocele repair a...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2015/709452

    authors: Ustuner M,Yilmaz H,Yavuz U,Ciftci S,Saribacak A,Aynur BS,Yasar H,Culha MM

    更新日期:2015-01-01 00:00:00

  • Current Concept and Update of the Macrophage Plasticity Concept: Intracellular Mechanisms of Reprogramming and M3 Macrophage "Switch" Phenotype.

    abstract::Macrophages play a key role in immunity. In this review, we consider the traditional notion of macrophage plasticity, data that do not fit into existing concepts, and a hypothesis for existence of a new switch macrophage phenotype. Depending on the microenvironment, macrophages can reprogram their phenotype toward the...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2015/341308

    authors: Malyshev I,Malyshev Y

    更新日期:2015-01-01 00:00:00

  • A new sampling method for spleen stiffness measurement based on quantitative acoustic radiation force impulse elastography for noninvasive assessment of esophageal varices in newly diagnosed HCV-related cirrhosis.

    abstract::In our study, we evaluated the feasibility of a new sampling method for splenic stiffness (SS) measurement by Quantitative Acoustic Radiation Force Impulse Elastography (Virtual Touch Tissue Quantification (VTTQ)).We measured SS in 54 patients with HCV-related cirrhosis of whom 28 with esophageal varices (EV), 27 with...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/365982

    authors: Rizzo L,Attanasio M,Pinzone MR,Berretta M,Malaguarnera M,Morra A,L'Abbate L,Balestreri L,Nunnari G,Cacopardo B

    更新日期:2014-01-01 00:00:00

  • Vitamin B-6 Supplementation Could Mediate Antioxidant Capacity by Reducing Plasma Homocysteine Concentration in Patients with Hepatocellular Carcinoma after Tumor Resection.

    abstract::Vitamin B-6 has a strong antioxidative effect. It would be useful to determine whether vitamin B-6 supplementation had effects on antioxidant capacities in patients with hepatocellular carcinoma (HCC) who had recently undergone tumor resection. Thirty-three HCC patients were randomly assigned to either the placebo (n ...

    journal_title:BioMed research international

    pub_type: 杂志文章,随机对照试验

    doi:10.1155/2016/7658981

    authors: Cheng SB,Lin PT,Liu HT,Peng YS,Huang SC,Huang YC

    更新日期:2016-01-01 00:00:00

  • Immunomodulation by Zearalenone in Carp (Cyprinus carpio L.).

    abstract::The mycotoxin zearalenone (ZEN) is a frequent contaminant of animal feeds, but its effects on fish have not yet been investigated extensively. In order to fill this gap a feeding trial with juvenile carp was conducted. Three groups of fish were fed feeds contaminated with ZEN at three concentrations (low: 332 μg kg(-1...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/420702

    authors: Pietsch C,Junge R,Burkhardt-Holm P

    更新日期:2015-01-01 00:00:00

  • HMMBinder: DNA-Binding Protein Prediction Using HMM Profile Based Features.

    abstract::DNA-binding proteins often play important role in various processes within the cell. Over the last decade, a wide range of classification algorithms and feature extraction techniques have been used to solve this problem. In this paper, we propose a novel DNA-binding protein prediction method called HMMBinder. HMMBinde...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2017/4590609

    authors: Zaman R,Chowdhury SY,Rashid MA,Sharma A,Dehzangi A,Shatabda S

    更新日期:2017-01-01 00:00:00

  • The Frequency and Spectrum of Chromosomal Translocations in a Cohort of Sri Lankans.

    abstract::Translocations are the most common type of structural chromosomal abnormalities. Unbalanced translocations are usually found in children who present with congenital abnormalities, developmental delay, or intellectual disability. Balanced translocations are usually found in adults who frequently present with reproducti...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/9797104

    authors: Paththinige CS,Sirisena ND,Kariyawasam UGIU,Dissanayake VHW

    更新日期:2019-04-02 00:00:00

  • Corrigendum to "Influence of a 10-Day Mimic of Our Ancient Lifestyle on Anthropometrics and Parameters of Metabolism and Inflammation: The "Study of Origin"".

    abstract::[This corrects the article DOI: 10.1155/2016/6935123.]. ...

    journal_title:BioMed research international

    pub_type: 已发布勘误

    doi:10.1155/2017/1641589

    authors: Pruimboom L,Ruiz-Núñez B,Raison CL,Muskiet FAJ,Freese J

    更新日期:2017-01-01 00:00:00

  • Seasonal Variation in Essential Oils Composition and the Biological and Pharmaceutical Protective Effects of Mentha longifolia Leaves Grown in Tunisia.

    abstract::This research assessed the seasonal variation of the chemical composition and antibacterial and anticholinesterase activities of essential oils extracted from M. longifolia leaves. The leaves organic fractions were also investigated for their biological activities and pharmacological functions. The essential oil highe...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/7856517

    authors: Zouari-Bouassida K,Trigui M,Makni S,Jlaiel L,Tounsi S

    更新日期:2018-12-09 00:00:00

  • Identifying Potentially Avoidable Emergency Department Visits of Long-Term Care Hospital Residents in Korea: A Multicenter Retrospective Cohort Study.

    abstract::The aims of this study were to investigate the reasons of transfers from long-term care hospitals (LTCHs) to emergency departments (EDs) of university hospitals in geriatric patients and to categorize the avoidable causes of these transfers. This retrospective multicenter study involved patients aged 65 years and olde...

    journal_title:BioMed research international

    pub_type: 杂志文章,多中心研究

    doi:10.1155/2019/7041607

    authors: Kim K,Lee DH,Yune HY,Wee JH,Kim DH,Kim EC,Lim JY,Choi SP

    更新日期:2019-06-19 00:00:00

  • Synthesis and Properties of pH-, Thermo-, and Salt-Sensitive Modified Poly(aspartic acid)/Poly(vinyl alcohol) IPN Hydrogel and Its Drug Controlled Release.

    abstract::Modified poly(aspartic acid)/poly(vinyl alcohol) interpenetrating polymer network (KPAsp/PVA IPN) hydrogel for drug controlled release was synthesized by a simple one-step method in aqueous system using poly(aspartic acid) grafting 3-aminopropyltriethoxysilane (KH-550) and poly(vinyl alcohol) (PVA) as materials. The h...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/236745

    authors: Lu J,Li Y,Hu D,Chen X,Liu Y,Wang L,Zhao Y

    更新日期:2015-01-01 00:00:00

  • Long-term effects of postearthquake distress on brain microstructural changes.

    abstract::Stressful events can have both short- and long-term effects on the brain. Our recent investigation identified short-term white matter integrity (WMI) changes in 30 subjects soon after the Japanese earthquake. Our findings suggested that lower WMI in the right anterior cingulum (Cg) was a pre-existing vulnerability fac...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/180468

    authors: Sekiguchi A,Kotozaki Y,Sugiura M,Nouchi R,Takeuchi H,Hanawa S,Nakagawa S,Miyauchi CM,Araki T,Sakuma A,Taki Y,Kawashima R

    更新日期:2014-01-01 00:00:00

  • Uptake of etoposide in CT-26 cells of colorectal cancer using folate targeted dextran stearate polymeric micelles.

    abstract::Targeted drug delivery using folate receptors is one of the most interesting chemotherapeutic research areas over the past few years. A novel folate targeted copolymer was synthesized using dextran stearate coupled to folic acid. FT-IR and NMR spectroscopy were used to confirm successful conjugation. Micelles prepared...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/708593

    authors: Varshosaz J,Hassanzadeh F,Sadeghi-Aliabadi H,Firozian F

    更新日期:2014-01-01 00:00:00

  • Variations in the Intragene Methylation Profiles Hallmark Induced Pluripotency.

    abstract::We demonstrate the potential of differentiating embryonic and induced pluripotent stem cells by the regularized linear and decision tree machine learning classification algorithms, based on a number of intragene methylation measures. The resulting average accuracy of classification has been proven to be above 95%, whi...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/976362

    authors: Druzhkov P,Zolotykh N,Meyerov I,Alsaedi A,Shutova M,Ivanchenko M,Zaikin A

    更新日期:2015-01-01 00:00:00

  • In Vitro Comparison of Fluoride, Magnesium, and Calcium Phosphate Materials on Prevention of White Spot Lesions around Orthodontic Brackets.

    abstract::One common negative side effect of orthodontic treatment with fixed appliances is the development of white spot lesions (WSLs) around brackets. This study is aimed at comparing the efficacy of various oral hygiene practices in preventing enamel demineralization around orthodontic brackets under similar in vitro condit...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/1989817

    authors: Lale S,Solak H,Hınçal E,Vahdettin L

    更新日期:2020-03-30 00:00:00

  • Short-Term Results Suggest That Sleeved Stomach without Resection Is as Effective as Sleeve Gastrectomy in Improving Glucose Control in Type 2 Diabetes Mellitus Sprague-Dawley Rat Model.

    abstract:Background:Although sleeve gastrectomy results in good weight loss and metabolic improvements, it is an irreversible procedure. Therefore, we attempted to assess the possibility of creating a sleeved stomach without resection. Material and Methods. A total of 22 male Sprague-Dawley rats with type 2 diabetes were random...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/9024923

    authors: Zhang W,Widjaja J,Yao L,Shao Y,Zhu X,Li C

    更新日期:2020-05-01 00:00:00

  • Clinical evaluation of CyberKnife in the treatment of vestibular schwannomas.

    abstract:OBJECTIVE:This study assessed the posttreatment tumor control and auditory function of vestibular schwannoma (VS) patients after CyberKnife (CK) and analyzed the possible prognostic factors of hearing loss. METHODS:We retrospectively studied 117 VS patients, with Gardner-Robertson (GR) classification grades I to IV, w...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/297093

    authors: Tsai JT,Lin JW,Lin CM,Chen YH,Ma HI,Jen YM,Chen YH,Ju DT

    更新日期:2013-01-01 00:00:00

  • The Usefulness of Performing Biochemical Tests in the Saliva of Kickboxing Athletes in the Dynamic of Training.

    abstract::The study aimed to determine the suitability of testing the saliva of kickboxing athletes to show changes in biochemical parameters in dynamic of training. 8 elite male athletes (mean age 17.29± 0.31 years, body mass 66.82± 3.46kg, with 5.62±0.96 years of training experience) participated in the study. Indicators of l...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/2014347

    authors: Volodchenko OA,Podrigalo LV,Iermakov SS,Żychowska MT,Jagiełło W

    更新日期:2019-01-10 00:00:00

  • Iron-Chelating Agent Can Maintain Bone Homeostasis Disrupted by Iron Overload by Upregulating Wnt/Beta-Catenin Signaling.

    abstract:Background:The incidence of osteoporotic fractures is increasing. In this study, we explored the activities of Wnt/β-catenin signaling in bone tissues with iron accumulation. Methods:We established rat bipedal walking models (RBWM), and a portion of our RBWM rats were intraperitoneally injected with ferric ammonium ci...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2020/8256261

    authors: Xu W,Yu R,Zhu X,Li Z,Jia J,Li D,Chen Y,Zhang X

    更新日期:2020-05-24 00:00:00

  • Diagnosis and Treatment of Human Salmonellosis in Addis Ababa City, Ethiopia.

    abstract:Background:Diagnosis using reliable tools and treatment following in vitro antimicrobial susceptibility tests are critical to proper addressing of antibiotic-resistant Salmonella infection. Methodology:A cross-sectional study was conducted to assess the practice of diagnosis and treatment of salmonellosis in Addis Aba...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/6406405

    authors: Garedew L,Solomon S,Worku Y,Worku H,Gemeda D,Lelissa G,Mamuye Y,Abubeker R,Mihret A,Fentaw S,Worku A,Bahiru M,Erenso G

    更新日期:2018-05-23 00:00:00

  • The usefulness of impedance cardiography for predicting beneficial effects of cardiac rehabilitation in patients with heart failure.

    abstract:AIM:Cardiac rehabilitation (CR) is an important part of heart failure (HF) treatment. The aim of this paper was to evaluate if thoracic fluid content (TFC) measured by impedance cardiography (ICG) is a useful parameter for predicting the outcome of CR. METHODS:Fifty HF patients underwent clinical and noninvasive haemo...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2013/595369

    authors: Gielerak G,Krzesiński P,Piotrowicz E,Piotrowicz R

    更新日期:2013-01-01 00:00:00

  • Evaluation of plant phenolic metabolites as a source of Alzheimer's drug leads.

    abstract::Epidemiological studies have proven an association between consumption of polyphenols and prevention of Alzheimer's disease, the most common form of dementia characterized by extracellular deposition of amyloid beta plaques. The aim of this study is pharmacological screening of the aqueous alcohol extract of Markhamia...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2014/843263

    authors: Hassaan Y,Handoussa H,El-Khatib AH,Linscheid MW,El Sayed N,Ayoub N

    更新日期:2014-01-01 00:00:00

  • Circulating Irisin Level and Thyroid Dysfunction: A Systematic Review and Meta-Analysis.

    abstract::Both thyroid hormones and irisin have profound influences on the metabolism of the human body. Based on their similarities, several studies have been conducted to explore changes in irisin levels in patients with hypothyroidism and hyperthyroidism. This study was conducted in accordance with the PRISMA statement and t...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2020/2182735

    authors: Shan D,Zou L,Liu X,Cai Y,Dong R,Hu Y

    更新日期:2020-10-28 00:00:00

  • Rapid-Onset Obesity with Hypoventilation, Hypothalamic, Autonomic Dysregulation, and Neuroendocrine Tumors (ROHHADNET) Syndrome: A Systematic Review.

    abstract:Background and Aim:ROHHADNET (rapid-onset obesity with hypoventilation, hypothalamic, autonomic dysregulation, neuroendocrine tumor) syndrome is a rare disease with grave outcome. Although early recognition is essential, prompt diagnosis may be challenging due to its extreme rarity. This study aimed to systematically r...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2018/1250721

    authors: Lee JM,Shin J,Kim S,Gee HY,Lee JS,Cha DH,Rim JH,Park SJ,Kim JH,Uçar A,Kronbichler A,Lee KH,Shin JI

    更新日期:2018-11-21 00:00:00

  • Angiopoietin-1 Promotes the Integrity of Neovascularization in the Subcutaneous Matrigel of Type 1 Diabetic Rats.

    abstract:Objective:This study aimed to investigate the effects of Ang-1 on neovascularization of diabetic organs by subcutaneous Matrigel angiogenesis model, established in type 1 diabetic rats. Methods:Ang-1 adenoviral vector was constructed. The rat model was established by STZ and divided into four group. The Matrigel was i...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2019/2016972

    authors: Li S,Zou H,Gong M,Chen Y,Yan X,Yu L,Yang Y

    更新日期:2019-01-09 00:00:00

  • Omega-3 Polyunsaturated Fatty Acids: The Way Forward in Times of Mixed Evidence.

    abstract::Almost forty years ago, it was first hypothesized that an increased dietary intake of omega-3 polyunsaturated fatty acids (PUFA) from fish fat could exert protective effects against several pathologies. Decades of intense preclinical investigation have supported this hypothesis in a variety of model systems. Several c...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2015/143109

    authors: Weylandt KH,Serini S,Chen YQ,Su HM,Lim K,Cittadini A,Calviello G

    更新日期:2015-01-01 00:00:00

  • Amplification of the insulin-like growth factor 1 receptor gene is a rare event in adrenocortical adenocarcinomas: searching for potential mechanisms of overexpression.

    abstract:CONTEXT:IGF1R overexpression appears to be a prognostic biomarker of metastatic pediatric adrenocortical tumors. However, the molecular mechanisms that are implicated in its upregulation remain unknown. Aim. To investigate the potential mechanisms involved in IGF1R overexpression. PATIENTS AND METHODS:We studied 64 ad...

    journal_title:BioMed research international

    pub_type: 临床试验,杂志文章

    doi:10.1155/2014/936031

    authors: Ribeiro TC,Jorge AA,Almeida MQ,Mariani BM,Nishi MY,Mendonca BB,Fragoso MC,Latronico AC

    更新日期:2014-01-01 00:00:00

  • The Omega-3 Fatty Acid Docosahexaenoic Acid Modulates Inflammatory Mediator Release in Human Alveolar Cells Exposed to Bronchoalveolar Lavage Fluid of ARDS Patients.

    abstract:BACKGROUND:This study investigated whether the 1 : 2 ω-3/ω-6 ratio may reduce proinflammatory response in human alveolar cells (A549) exposed to an ex vivo inflammatory stimulus (bronchoalveolar lavage fluid (BALF) of acute respiratory distress syndrome (ARDS) patients). Methods. We exposed A549 cells to the BALF colle...

    journal_title:BioMed research international

    pub_type: 杂志文章

    doi:10.1155/2015/642520

    authors: Cotogni P,Trombetta A,Muzio G,Maggiora M,Canuto RA

    更新日期:2015-01-01 00:00:00

  • Maternal Overnutrition Programs Central Inflammation and Addiction-Like Behavior in Offspring.

    abstract::Obesity or maternal overnutrition during pregnancy and lactation might have long-term consequences in offspring health. Fetal programming is characterized by adaptive responses to specific environmental conditions during early life stages. Programming alters gene expression through epigenetic modifications leading to ...

    journal_title:BioMed research international

    pub_type: 杂志文章,评审

    doi:10.1155/2018/8061389

    authors: Montalvo-Martínez L,Maldonado-Ruiz R,Cárdenas-Tueme M,Reséndez-Pérez D,Camacho A

    更新日期:2018-06-20 00:00:00