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Molecular identification and functional characterization of a tumor necrosis factor (TNF) gene in Crassostrea hongkongensis.

Abstract：

:Tumor necrosis factor superfamily (TNFSF) represents a group of multifunctional inflammatory cytokines that have been shown to participate in a variety of pathological and immunological process. However, the functions of these proteins in oyster are still poorly understood. In the present study, an oyster TNF homolog (named ChTNF) was identified from a cDNA library of Crassostrea hongkongensis. The complete cDNA of ChTNF was 2457bp in length containing an open reading frame (ORF) of 1044bp, a 5'-untranslated region (UTR) of 381bp and a 3'-UTR of 1032bp. The deduced ChTNF protein consisted of 347 amino acids with a characteristic transmembrane domain and a typical TNF homology domain (THD). Quantitative real-time PCR analysis revealed that ChTNF was broadly expressed in various oyster tissues and different stages of embryonic development. In addition, transcriptional analysis indicated that ChTNF transcription levels in hemocytes were increased significantly in pathogen challenge groups (Vibrio alginolyticus and Staphylococcus haemolyticus) compared to that in the control. Moreover, in vitro PAMP (lipopolysaccharide and peptidoglycan) treatments showed a stimulatory effect on the expression of ChTNF in the primary cultured hemocytes of C. hongkongensis. Finally, dual-luciferase reporter assays revealed that ChTNF could activate the NF-κB-Luc reporter in a dose-dependent manner in HEK293T cells. Altogether, these findings may provide valuable information regarding oyster TNFs and its role in innate immunity.

journal_name

Immunobiology

journal_title

Immunobiology

authors

Qu F,Xiang Z,Zhang Y,Li J,Xiao S,Zhang Y,Qin Y,Zhou Y,Yu Z

doi

10.1016/j.imbio.2017.02.002

subject

Has Abstract

pub_date

2017-05-01 00:00:00

pages

751-758

issue

5

eissn

0171-2985

issn

1878-3279

pii

S0171-2985(17)30018-9

journal_volume

222

pub_type

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