Abstract:
:Dogs with lymphoma are established as good model for human non-Hodgkin lymphoma studies. Canine cell lines derived from lymphomas may be valuable tools for testing new therapeutic drugs. In this context, we established a canine T-cell line, PER-VAS, from a primary aggressive T-cell lymphoma with large granular morphology. Flow cytometric analysis revealed a stable immunophenotype: PER-VAS cells were positively labelled for CD5, CD45, MHC II and TLR3, and were negative for CD3, CD4 and CD8 expression. Although unstable along the culture process, IL-17 and MMP12 proteins were detectable as late as at passages 280 and 325i.e. respectively 24 and 29 months post isolation. At passage 325, PER-VAS cells maintained the expression of IL-17, CD3, CD56, IFNγ and TNFα mRNAs as shown by RT-PCR analysis. Stable rearrangement of the TCRγ gene has been evidenced by PCR. PER-VAS cells have a high proliferation index with a doubling time of 16.5h and were tumorigenic in Nude mice. Compared to the canine cell lines already reported, PER-VAS cells display an original expression pattern, close to NKT cells, which makes them valuable tools for in vitro comparative research on lymphomas.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Bonnefont-Rebeix C,Fournel-Fleury C,Ponce F,Belluco S,Watrelot D,Bouteille SE,Rapiteau S,Razanajaona-Doll D,Pin JJ,Leroux C,Marchal Tdoi
10.1016/j.imbio.2015.08.007subject
Has Abstractpub_date
2016-01-01 00:00:00pages
12-22issue
1eissn
0171-2985issn
1878-3279pii
S0171-2985(15)30047-4journal_volume
221pub_type
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