当前位置： SCI文献检索 > IMMUNOBIOLOGY期刊下所有文献 > An advanced approach for the characterization of dendritic cell-induced T cell proliferation in situ.

An advanced approach for the characterization of dendritic cell-induced T cell proliferation in situ.

Abstract：

:It is commonly accepted that dendritic cells (DCs) play a pivotal role in the induction of adaptive T cell-mediated immune responses. The clonal expansion of antigen-specific T cells within secondary lymphoid organs reflects the efficiency of antigen processing and presentation by DCs. Consequently, the quantification of proliferating antigen-specific T cells represents an important read-out for analyzing DC-T cell interactions. Standard proliferation assays are usually performed with cell suspensions of antigen-stimulated lymphocytes labelled with carboxyfluorescein diacetate succinimidyl ester, [(3)H] thymidine, or 5-bromo-2-deoxyuridine. However, these assays have important limitations, including the complete loss of information about the localization of proliferating cells in situ. Additionally, the ex vivo stimulation with antigen does not necessarily reflect the in vivo situation of cell proliferation. Therefore, an advanced approach for a detailed characterization of proliferating T cells in situ would be helpful for the interpretation of ongoing adaptive immune responses. Here, we describe the development of a fluorescence-based multicolour assay allowing the characterization and quantification of proliferation in different T cell subtypes in cryosections of lymphoid organs. Our approach combines the major benefits of flow cytometry and immunofluorescent-based histology, such as (i) multicolour phenotyping of cell populations and (ii) description of tissue-specific sites of cell proliferation.

journal_name

Immunobiology

journal_title

Immunobiology

authors

Florian C,Barth T,Wege AK,Männel DN,Ritter U

doi

10.1016/j.imbio.2010.05.017

subject

Has Abstract

pub_date

2010-09-01 00:00:00

pages

855-62

issue

9-10

eissn

0171-2985

issn

1878-3279

pii

S0171-2985(10)00083-5

journal_volume

215

pub_type

杂志文章

相关文献

IMMUNOBIOLOGY文献大全
  • DAP10 contributes to CD8(+) T cell-mediated cytotoxic effector mechanisms during Mycobacterium tuberculosis infection.

    abstract：:The activating C-type lectin-like receptor NKG2D, which is expressed by mouse NK cells and activated CD8 T cells, was previously demonstrated to be involved in tumor rejection and as a defense mechanism against viral and bacterial infections. Because CD8 T cells are important for protective immune responses during chr...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2010.09.010

    authors： Hessmann M,Rausch A,Rückerl D,Adams PS,Simon M,Gilfillan S,Colonna M,Ehlers S,Hölscher C

    更新日期：2011-05-01 00:00:00

  • BCG immunomodulation: From the 'hygiene hypothesis' to COVID-19.

    abstract：:The century-old tuberculosis vaccine BCG has been the focus of renewed interest due to its well-documented ability to protect against various non-TB pathogens. Much of these broad spectrum protective effects are attributed to trained immunity, the epigenetic and metabolic reprogramming of innate immune cells. As BCG v...

    journal_title：Immunobiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.imbio.2020.152052

    authors： Moulson AJ,Av-Gay Y

    更新日期：2020-12-24 00:00:00

  • Phagocyte recognition by lectin receptors.

    abstract：:A "lectin-like" receptor for bacterial cell wall sugars, originally described in the membrane of mouse peritoneal macrophages, which facilitates binding of various bacteria to the macrophage surface, has now been shown to be present on most phagocytes from a variety of species. The role of such a receptor in the infla...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(82)80090-9

    authors： Weir DM,Stewart J,Glass E

    更新日期：1982-04-01 00:00:00

  • Enriched natural killing and antibody-dependent cellular cytotoxicity in lymphocyte populations adherent to tumor cell monolayers.

    abstract：:We have previously shown that human peripheral blood lymphocytes that do not adhere to natural killer (NK)-sensitive target cell monolayers are at least partially depleted of NK activity. To demonstrate directly that NK effector cells adhere to the monolayers, we have now recovered the adherent populations by treatmen...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(82)80052-1

    authors： Jensen PJ,Koren HS

    更新日期：1982-05-01 00:00:00

  • Induction of tolerance to non-H-2 alloantigens is not restricted by the MHC molecules expressed on the donor cells in cyclophosphamide-induced tolerance.

    abstract：:A long-lasting skin tolerance to non-H-2 alloantigens has been reported to be readily induced, when the recipient mice were primed intravenously (i.v.) with 5 x 10(7) spleen cells (SC) plus 1.5 x 10(7) bone marrow cells (BMC) from the H-2 identical strains of mice, and treated intraperitoneally (i.p.) with 200 mg/kg C...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(11)80511-5

    authors： Tomita Y,Nomoto K

    更新日期：1990-11-01 00:00:00

  • RNA interference mediated silencing of Hsp60 gene in human monocytic myeloma cell line U937 revealed decreased dengue virus multiplication.

    abstract：:Heat shock proteins (Hsps) or stress proteins are highly conserved molecules and expressed in all cell types under stressful conditions like heat, cold, hypoxia and infections. The objective of the present study was to determine the effect of dengue virus infection on relative expression of stress proteins and their r...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2008.11.010

    authors： Padwad YS,Mishra KP,Jain M,Chanda S,Karan D,Ganju L

    更新日期：2009-01-01 00:00:00

  • Dendritic cells shuttle microbes across gut epithelial monolayers.

    abstract：:Understanding the mechanisms governing the type of induced immune response after microbial invasion, could be of crucial importance for the rational design of a bacteria-based vaccine. Targeting a vaccine directly to dendritic cells (DCs), which are considered the most powerful antigen presenting cells, could be extre...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1078/0171-2985-00094

    authors： Rescigno M,Rotta G,Valzasina B,Ricciardi-Castagnoli P

    更新日期：2001-12-01 00:00:00

  • Crosstalk between glucocorticoids and IL-4 modulates Ym1 expression in alternatively activated myeloid cells.

    abstract：:Airway epithelial cells induce a tolerogenic microenvironment by modulating immune cells in the lung. We recently showed that the supernatant of airway epithelial cells induces two marker genes of alternative activation, Ym1 and Ms4a8a, in respiratory myeloid cells. This induction was partially mediated by glucocortic...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2017.02.003

    authors： Ng Kuet Leong N,Brombacher F,Dalpke AH,Weitnauer M

    更新日期：2017-05-01 00:00:00

  • The in vitro activity of lectin I from mistletoe (ML I) and its isolated A and B chains on functions of macrophages and polymorphonuclear cells.

    abstract：:We investigated the effects of ML I and its isolated chains, A and B, in regard to selected functions of phagocytes (human granulocytes, paraffin-oil stimulated M phi from guinea pigs). On these cells, ML I has no cytotoxic effect between 10(-14) and 10(-8) (trypan blue exclusion and ethidium bromide exclusion). Over ...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(85)80002-4

    authors： Metzner G,Franz H,Kindt A,Fahlbusch B,Süss J

    更新日期：1985-07-01 00:00:00

  • The potential role of subclinical Bordetella Pertussis colonization in the etiology of multiple sclerosis.

    abstract：:It is established that (1) subclinical Bordetella pertussis colonization of the nasopharynx persists in highly vaccinated populations, and (2) B. pertussis toxin is a potent adjuvant that, when co-administered with neural antigens, induces neuropathology in experimental autoimmune encephalomyelitis, the principle anim...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2015.12.008

    authors： Rubin K,Glazer S

    更新日期：2016-04-01 00:00:00

  • Generation of a novel Cr2 gene allele by homologous recombination that abrogates production of Cr2 but is sufficient for expression of Cr1.

    abstract：:The enhancing effects of the complement system for humoral immunity have primarily focused upon the recognition of complement-bound foreign antigens by a co-receptor complex of the antigen-specific B cell receptor (BCR) and complement receptor 2 (Cr2). In vivo experiments using Cr2 gene deficient mice (which lack the ...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2013.08.003

    authors： Donius LR,Orlando CM,Weis JJ,Weis JH

    更新日期：2014-01-01 00:00:00

  • The TNF-α of mast cells induces pro-inflammatory responses during infection with Acinetobacter baumannii.

    abstract：:Mast cells serve important roles as sentinels against bacterial infection by secreting mediators stored in granules. Much of their effectiveness depends upon recruiting and/or modulating other immune cells. The location of mast cells implies that they recognize pathogens invading tissues or mucosal tissues. Acinetobac...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2017.05.015

    authors： Kikuchi-Ueda T,Kamoshida G,Ubagai T,Nakano R,Nakano A,Akuta T,Hikosaka K,Tansho-Nagakawa S,Kikuchi H,Ono Y

    更新日期：2017-11-01 00:00:00

  • Optimal conditions for the preparation of ferritin-labeled antibodies defined by binding to their antigen in an ELISA.

    abstract：:An ELISA with covalently fixed antigens was used to measure and define ferritin-labeled antibodies. In the presented model, RIG covalently fixed to glass tubes served as antigen. Twenty ferritin-labeled SWaRIG conjugates were prepared with different molar ratios of antibody: ferritin: glutaraldehyde. At various diluti...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(84)80021-2

    authors： Rauterberg EW,Schieck C,Kreft H,Römer W

    更新日期：1984-05-01 00:00:00

  • A common immunological epitope existing between HIV-1 gp41 and human interferon-alpha and -beta.

    abstract：:Based on the similar effects that HIV-1 gp41 like human type I interferons (IFN-alpha and -beta) upregulated MHC expression and inhibited lymphocyte proliferation, we compared amino acid sequences of gp41 and human type I interferons (IFN-alpha and -beta), and found sequence-similarity existing between the immunosuppr...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/s0171-2985(98)80043-0

    authors： Chen YH,Dierich MP

    更新日期：1998-02-01 00:00:00

  • Administration of anti-CD25 mAb leads to impaired α-galactosylceramide-mediated induction of IFN-γ production in a murine model.

    abstract：:CD4(+)CD25(+)Foxp3(+) T regulatory cells (Tregs) and CD1d-restricted invariant natural killer T (iNKT) cells are two cell types that are known to regulate immune reactions. Depletion or inactivation of Tregs using specific anti-CD25 antibodies in combination with immunostimulation is an attractive modality especially ...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2012.10.012

    authors： Rosalia RA,Štěpánek I,Polláková V,Šímová J,Bieblová J,Indrová M,Moravcová S,Přibylová H,Bontkes HJ,Bubeník J,Sparwasser T,Reiniš M

    更新日期：2013-06-01 00:00:00

  • Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets.

    abstract：:Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can act...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2015.07.018

    authors： Cortés-Garcia JD,López-López C,Cortez-Espinosa N,García-Hernández MH,Guzmán-Flores JM,Layseca-Espinosa E,Portales-Cervantes L,Portales-Pérez DP

    更新日期：2016-01-01 00:00:00

  • Cytokine treatment of macrophage suppression of T cell activation.

    abstract：:High Mphi:T cell ratios suppress the immune response to the retroviral superantigen Mls by IFNgamma-triggered production of the arg- and trp-consuming enzymes iNOS and IDO. Attempts to reverse suppression by treatment with pro-inflammatory cytokines revealed that IL-6 improved the T cell response to Mls and the pro-he...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2009.01.015

    authors： Silberman D,Bucknum A,Kozlowski M,Matlack R,Riggs J

    更新日期：2010-01-01 00:00:00

  • Monocyte-derived macrophages matured under prolonged hypoxia transcriptionally up-regulate HIF-1α mRNA.

    abstract：:This study tested the hypothesis that prolonged severe hypoxia during monocyte to macrophage differentiation results in macrophages with a pattern of gene expression and phenotype distinct from those maturing in normal oxygen levels. Macrophages accumulate in hypoxic and anoxic areas within pathological sites such as ...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2010.12.005

    authors： Staples KJ,Sotoodehnejadnematalahi F,Pearson H,Frankenberger M,Francescut L,Ziegler-Heitbrock L,Burke B

    更新日期：2011-07-01 00:00:00

  • Soluble macrophage-derived CD163: a homogenous ectodomain protein with a dissociable haptoglobin-hemoglobin binding.

    abstract：BACKGROUND:The soluble form of the haptoglobin-hemoglobin (Hp-Hb) scavenger receptor (sCD163) is a specific plasma/serum marker for macrophage activity. Here, we have characterized molecular forms in serum and investigated a role of sCD163 as a binder of Hp-Hb complexes. METHODS:The sCD163 species in serum (from 50 he...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2009.05.003

    authors： Møller HJ,Nielsen MJ,Maniecki MB,Madsen M,Moestrup SK

    更新日期：2010-05-01 00:00:00

  • Heterogeneity of the Mac-1 expression on peripheral blood neutrophils in patients with different types of epithelial ovarian cancer.

    abstract：:The expression level of Mac-1 on the surface of neutrophils is an important indicator of neutrophil activation. Under pathological conditions, Mac-1 is believed a key adhesion molecule that facilitates cancer progression and mediates the adhesion of tumour cells to the endothelium of blood vessels. Our previous findin...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2015.10.003

    authors： Bednarska K,Klink M,Wilczyński JR,Szyłło K,Malinowski A,Sułowska Z,Nowak M

    更新日期：2016-02-01 00:00:00

  • Clonal analysis of the synergistic mitogenic effect of lipopolysaccharide and dextran sulphate on B cell activation, growth, and differentiation into Ig-secreting cells.

    abstract：:Splenic B cells of BALB/c mice were stimulated in vitro either with lipopolysaccharide (LPS), dextran sulphate (DxS), or with both LPS and DxS. The absolute frequency of B cells that differentiate into clones of immunoglobulin (Ig)-secreting cells upon activation with these mitogens was determined by means of limiting...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/s0171-2985(88)80060-3

    authors： Bos NA,Meeuwsen CG,de Visser H,Benner R

    更新日期：1988-02-01 00:00:00

  • Increased cortisol levels in human umbilical cord blood inhibit interferon alpha production of neonates.

    abstract：:Immunosuppressive effects of corticosteroids are generally known, but the mechanisms influencing the immune system are poorly understood. However, a correlation between cortisol levels and cytokine production has been reported by several investigators. In the present study we determined cortisol concentrations in huma...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(99)80072-2

    authors： Reissland P,Wandinger KP

    更新日期：1999-06-01 00:00:00

  • Immunofluorescence studies on the codistribution immune deposits and complement in the thyroid glands of Obese strain (OS) chickens.

    abstract：:This study demonstrates immune complexes in thyroid glands of Obese strain (OS) chickens, that consist of thyroglobulin (Tg) and antibodies to Tg. In IIF tests it was shown that these complexes fix complement with an age-dependent increase from 12% in 19-day-old embryos up to 100% in 6-week-old animals. This finding a...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/S0171-2985(83)80035-7

    authors： Kofler H,Kofler R,Wolf H,Wick G

    更新日期：1983-05-01 00:00:00

  • Up-regulation of iNOS mRNA expression and increased production of NO in human monoblast cell line, U937 transfected by HTLV-I tax gene.

    abstract：:We investigated the mRNA expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) in human T cell lymphotropic virus type I (HTLV-I) p40tax-transfected U937 cells, a human monoblast cell line. Transfection of HTLV-I p40tax U937 cells induced up-regulation of iNOS mRNA expression and...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/s0171-2985(97)80083-6

    authors： Goto H,Nakamura T,Shirabe S,Ueki Y,Nishiura Y,Furuya T,Tsujino A,Nakane S,Eguchi K,Nagataki S

    更新日期：1997-11-01 00:00:00

  • Activation of the immune response against Infectious Bursal Disease Virus after intramuscular inoculation of an intermediate strain.

    abstract：:Infectious bursal disesase is a highly contagious, wide spread immunosuppressive chicken disease caused by the Infectious Bursal Disease Virus (IBDV). IBDV is a two segmented double-strand RNA virus, member of the Birnaviridae family. In order to study the interaction between IBDV and the immune system, chickens were ...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2011.03.003

    authors： Carballeda JM,Zoth SC,Gómez E,Gravisaco MJ,Berinstein A

    更新日期：2011-09-01 00:00:00

  • Generation of anti-Neu-glycolyl-ganglioside antibodies by immunization with an anti-idiotype monoclonal antibody: A self versus non-self-matter.

    abstract：:We have previously generated a murine anti-idiotype (Ab2) monoclonal antibody (mAb) to a murine Ab1 mAb, named P3, which selectively binds Neu-glycolyl (NeuGc)-sialic acid on several monosialo- and disialogangliosides, and also reacts with sulfatides and antigens expressed in human melanoma and breast tumors. This Ab2...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2005.02.002

    authors： Hernández AM,Rodríguez M,López-Requena A,Beausoleil I,Pérez R,Vázquez AM

    更新日期：2005-01-01 00:00:00

  • UL16 binding proteins.

    abstract：:According to present concepts, innate immunity plays an important role in tumor surveillance and immune modulation. The state of NK cells depends on the balance between inhibitory and activating signals from corresponding receptors. As one of the activating receptors, NKG2D recognises some self ligands such as MICA/B ...

    journal_title：Immunobiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.imbio.2004.04.008

    authors： Cao W,He W

    更新日期：2004-01-01 00:00:00

  • Cyclophosphamide-induced immunological tolerance: an overview.

    abstract：:A cyclophosphamide (CP)-induced tolerance system in mice that primarily consists of donor cell injection followed by CP-treatment was found useful for inducing a long-lasting allo- or xeno-tolerance to various solid organs. In the cells-followed-by-CP system, the sequential mechanisms of tolerance were clarified using...

    journal_title：Immunobiology

    pub_type： 杂志文章,评审

    doi：10.1016/S0171-2985(96)80033-7

    authors： Mayumi H,Umesue M,Nomoto K

    更新日期：1996-07-01 00:00:00

  • Enteric reovirus infection stimulates peanut-specific IgG2a responses in a mouse food allergy model.

    abstract：:IgE-mediated food allergies are an important cause of life-threatening hypersensitivity reactions. Orally administered peanut antigens mixed with the mucosal adjuvant cholera toxin (CT) induce a strong peanut extract (PE)-specific serum IgE response that is correlated with T-helper type 1 (Th1) and type 2 (Th2)-like T...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2010.02.004

    authors： Fecek RJ,Marcondes Rezende M,Busch R,Hassing I,Pieters R,Cuff CF

    更新日期：2010-12-01 00:00:00

  • Acute immunomodulatory effects of iron polyisomaltosate in rats.

    abstract：:Anti-anaemic drug ferric-sorbitol citrate showed immunomodulatory effects, activating NF-kappaB in peritoneal macrophages, which consequently secrete tumour necrosis factor-alpha (TNF-alpha). TNF-alpha activates NF-kappaB in spleen cells. The aim of this study was to investigate the effect of iron polyisomaltosate, an...

    journal_title：Immunobiology

    pub_type： 杂志文章

    doi：10.1016/j.imbio.2008.07.005

    authors： Poljak-Blazi M,Jaganjac M,Mustapic M,Pivac N,Muck-Seler D

    更新日期：2009-01-01 00:00:00