Abstract:
:S. epidermidis is responsible for biofilm-related nonunions. This study compares the response to S. epidermidis-infected fractures in rats systemically or locally injected with vancomycin or bone marrow mesenchymal stem cells (BMSCs) in preventing the nonunion establishment. The 50% of rats receiving BMSCs intravenously (s-rBMSCs) died after treatment. A higher cytokine trend was measured in BMSCs locally injected rats (l-rBMSCs) at day 3 and in vancomycin systemically injected rats (l-VANC) at day 7 compared to the other groups. At day 14, the highest cytokine values were measured in l-VANC and in l-rBMSCs for IL-10. µCT showed a good bony bridging in s-VANC and excellent both in l-VANC and in l-rBMSCs. The bacterial growth was lower in s-VANC and l-VANC than in l-rBMSCs. Histology demonstrated the presence of new woven bone in s-VANC and a more mature bony bridging was found in l-VANC. The l-rBMSCs showed a poor bony bridging of fibrovascular tissue. Our results could suggest the synergic use of systemic and local injection of vancomycin as an effective treatment to prevent septic nonunions. This study cannot sustain the systemic injection of BMSCs due to high risks, while a deeper insight into local BMSCs immunomodulatory effects is mandatory before developing cell therapies in clinics.
journal_name
Mediators Inflammjournal_title
Mediators of inflammationauthors
Lovati AB,Drago L,Bottagisio M,Bongio M,Ferrario M,Perego S,Sansoni V,De Vecchi E,Romanò CLdoi
10.1155/2016/9595706subject
Has Abstractpub_date
2016-01-01 00:00:00pages
9595706eissn
0962-9351issn
1466-1861journal_volume
2016pub_type
杂志文章abstract::Methyltransferase-like 3 (METTL3), an RNA N6-methyladenosine (m6A) methyltransferase, is essential for the m6A mRNA modification. As a key enzyme of m6A methylation modification, METTL3 has been implicated in immune and inflammation regulation. However, little is known of the role and underlying mechanism of METTL3 in...
journal_title:Mediators of inflammation
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abstract::Observation of the microcirculation of the hamster cheek pouch by intravital microscopy revealed five steps of neutrophil migration from the venules after topical application of leukotriene B(4) to the microvasculature: rolling along the venular wall (Step 1), adhesion to it (Step 2), disappearance from the vascular l...
journal_title:Mediators of inflammation
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abstract::Stimulation of phosphoinositide hydrolysis in myocardium from autoimmune myocarditis mice by ThEA and histamine was assayed. Myocardium from autoimmune heart, but not the normal forms, specifically increased phosphoinositide turnover in the presence of histaminergic agonists. This increment was blocked by a specific H...
journal_title:Mediators of inflammation
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journal_title:Mediators of inflammation
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abstract::Autism is a neurodevelopmental disorder characterized by problems in communication, social skills, and repetitive behavior. Recent studies suggest that apoptotic and inflammatory mechanisms may contribute to the pathogenesis of this disorder. Nuclear factor-κB (NF-κB) is an important gene transcriptional factor involv...
journal_title:Mediators of inflammation
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abstract::To investigate the role of AEG-1 in glycolysis and tumorigenesis, we construct myc-AEG-1 expression vector and demonstrate a novel mechanism that AEG-1 may increase the activity of AMPK by Thr172 phosphorylation. The higher expression levels of AEG-1 in colorectal carcinoma cells were found but showed significant diff...
journal_title:Mediators of inflammation
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abstract::The number of persons who relocate to regions of high altitude for work, pleasure, sport, or residence increases every year. It is known that the reduced supply of oxygen (O2) induced by acute or chronic increases in altitude stimulates the body to adapt to new metabolic challenges imposed by hypoxia. Sleep can suffer...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00
abstract::Interleukin-5 (IL-5) is a cytokine that preferentially effects the development and function of eosinophils, and is considered important in the pathophysiology of allergic inflammation. In this study, we evaluated the ability of recombinant human IL-5 (rHu IL-5) to promote tissue eosinophilia and the importance of this...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935195000536
更新日期:1995-01-01 00:00:00
abstract::Chronic pain has to be considered in all respects a debilitating disease and 10-20% of the world's adult population is affected by this disease. In the most general terms, pain is symptomatic of some form of dysfunction and (often) the resulting inflammatory processes in the body. In the study of pain, great attention...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/183041
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abstract::Serine proteinases have been recognized as playing an important role in inflammation via proteinase activated receptors (PARs). However, little is known about the influence of serine proteinases and PARs on TNF secretion from highly purified T cells. We challenged T cells from human peripheral blood with serine protei...
journal_title:Mediators of inflammation
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doi:10.1155/2013/165453
更新日期:2013-01-01 00:00:00
abstract:Objective:Renal ischemia/reperfusion injury (RI/RI) is the main cause of acute kidney injury. Total glucosides of paeony (TGP) are a traditional Chinese medicine. This study was aimed at exploring the role of TGP in RI/RI and its underlying mechanism of action. Methods:Rat RI/RI models were constructed by surgical ope...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2020/8869511
更新日期:2020-11-24 00:00:00
abstract::Lactobacillus species can exert health promoting effects in the gastrointestinal tract (GIT) through many mechanisms, which include pathogen inhibition, maintenance of microbial balance, immunomodulation, and enhancement of the epithelial barrier function. Different species of the genus Lactobacillus can evoke differe...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/237921
更新日期:2013-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effects and mechanisms of hydroxytyrosol (HT) during the pathogenesis of myocardial ischemia reperfusion (I/R) in rat hearts. METHODS:The rats were randomized into five groups: sham group, I/R group, HT+I/R group, HT+LY294002+I/R group, and LY+I/R group. Myocardial infarct size, markers of...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2016/1232103
更新日期:2016-01-01 00:00:00
abstract::In respiratory diseases, there is an increased expression of multiple inflammatory proteins in the respiratory tract, including cytokines, chemokines, and adhesion molecules. Chemokines have been shown to regulate inflammation and immune cell differentiation. Moreover, many of the known inflammatory target proteins, s...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2013/791231
更新日期:2013-01-01 00:00:00
abstract::Monocyte chemotactic protein (MCP-1) is a specific monocyte chemoattractant and activating factor produced by both immune cells (mononuclear phagocytes and lymphocytes) and non-immune cells (parenchymal and stromal cells). In order to define the conditions under which human monocytes express MCP-1, monocytes were expo...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/S0962935194000050
更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Platelet-activating factor (PAF) seems to be implicated in systemic lupus erythematosus (SLE) patients with associated renal diseases. AIMS:In this study, we ensured the role of PAF in SLE patients without renal complications. METHODS:Blood PAF and acetylhydrolase activity, plasma soluble phospholipase A(2...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/0962935031000097718
更新日期:2003-04-01 00:00:00
abstract::Background. Patients with UC have shown an important defect in the secretion and maintenance of the mucosal barrier as part of inadequate expression of mucin genes. The aim of the present study was to determine the expression of MUC12, MUC16, and MUC20 in colonic tissue from patients with UC in regard to their clinica...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2015/659018
更新日期:2015-01-01 00:00:00
abstract::In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN- γ have been observed in peripheral blood. The balance between Th1, Th17, and FoxP3(+) CD4(+) T cell subsets in sarcoidosis remains unclear. Bronchoalveolar lavage fluid ce...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2014/438070
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:In this work we studied the correlation between platelet count, platelet activation, and systemic inflammation in overweight, obese, and morbidly obese individuals. METHODS AND SUBJECTS:A total of 6319 individuals participated in the study. Complete blood counts, high sensitivity C-reactive protein (hs-CRP) ...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2008/834153
更新日期:2008-01-01 00:00:00
abstract::The serum concentration of two pro-angiogenic cytokines: basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGF-beta1), and anti-angiogenic factor endostatin in the serum of 80 never treated B-cell chronic lymphocytic leukemia (CLL) patients and 27 healthy volunteers was measured using an enzy...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1080/0962935031000134888
更新日期:2003-06-01 00:00:00
abstract:BACKGROUND:We have previously reported a continuous activation of caspase-1 and increased interleukin (IL)-1β levels in early rheumatoid arthritis (RA). These observations raised the hypothesis that drugs targeting the IL-1β pathway, in addition to tumour necrosis factor (TNF), may be particularly effective for early R...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2014/195327
更新日期:2014-01-01 00:00:00
abstract::Group A streptococcus (GAS) imposes a great burden on humans. Efforts to minimize the associated morbidity and mortality represent a critical issue. Glycogen synthase kinase-3 β (GSK-3 β) is known to regulate inflammatory response in infectious diseases. However, the regulation of GSK-3 β in GAS infection is still unk...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2013/720689
更新日期:2013-01-01 00:00:00
abstract::Chronic inflammation has an important role in the development and progression of most fibrotic diseases, for which no effective treatments exist. Tubulointerstitial fibrosis (TF) is characterized by irreversible deposition of fibrous tissue in chronic kidney diseases. Prolonged injurious stimuli and chronic inflammati...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2018/5103672
更新日期:2018-10-14 00:00:00
abstract::Exosomes are nanosized vesicles released from every cell in the body including those in the respiratory tract and lungs. They are found in most body fluids and contain a number of different biomolecules including proteins, lipids, and both mRNA and noncoding RNAs. Since they can release their contents, particularly mi...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2016/5628404
更新日期:2016-01-01 00:00:00
abstract::Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause. The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression. When compared to healthy donors, SSTR5 was over-expressed 417 +/...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2009/285812
更新日期:2009-01-01 00:00:00
abstract::It was recently demonstrated that TLR4 activation via dietary lipids triggers inflammatory pathway and alters insulin responsiveness in the fat tissue during obesity. Here, we question whether other TLR family members could participate in the TLR-mediated inflammatory processes occurring in the obese adipose tissue. W...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2010/823486
更新日期:2010-01-01 00:00:00
abstract::The acute respiratory distress syndrome (ARDS), a clinical complication of severe acute lung injury (ALI) in humans, is a leading cause of morbidity and mortality in critically ill patients. Despite decades of research, few therapeutic strategies for clinical ARDS have emerged. Here we carefully evaluated the effect o...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2012/540794
更新日期:2012-01-01 00:00:00
abstract:AIMS:This retrospective analysis was aimed at evaluating the effectiveness of treatment of persistent diabetic macular edema with intravitreal injections of 0.7 mg dexamethasone implant Ozurdex. The study comprised three male patients (6 eyes). RESULTS:The average thickness of the retina at baseline was 632 μm, the m...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2014/364143
更新日期:2014-01-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are usually small molecule peptides, which display broad-spectrum antimicrobial activity, high efficiency, and stability. For the multiple-antibiotic-resistant strains, AMPs play a significant role in the development of novel antibiotics because of their broad-spectrum antimicrobial activ...
journal_title:Mediators of inflammation
pub_type: 杂志文章,评审
doi:10.1155/2015/167572
更新日期:2015-01-01 00:00:00
abstract::Leptin participates in the inflammatory responses in multiple cell types and animal models. Chronic cerebral hypoperfusion (CCH) induces inflammation in the central nervous system (CNS), which acts as one of the main reasons for CCH-induced white matter lesions (WMLs). But whether leptin participates in the pathogenes...
journal_title:Mediators of inflammation
pub_type: 杂志文章
doi:10.1155/2020/7974537
更新日期:2020-12-18 00:00:00