Differential between protein and mRNA expression of CCR7 and SSTR5 receptors in Crohn's disease patients.

Abstract:

:Crohn's disease (CD) is a multifactorial chronic inflammatory bowel disease of unknown cause. The aim of the present study was to explore if mRNA over-expression of SSTR5 and CCR7 found in CD patients could be correlated to respective protein expression. When compared to healthy donors, SSTR5 was over-expressed 417 +/- 71 times in CD peripheral blood mononuclear cells (PBMCs). Flow cytometry experiments showed no correlation between mRNA and protein expression for SSTR5 in PBMCs. In an attempt to find a reason of such a high mRNA expression, SSTR5 present on CD PBMCs were tested and found as biologically active as on healthy cells. In biopsies of CD intestinal tissue, SSTR5 was not over-expressed but CCR7, unchanged in PBMCs, was over-expressed by 10 +/- 3 times in the lamina propria. Confocal microscopy showed a good correlation of CCR7 mRNA and protein expression in CD intestinal biopsies. Our data emphasize flow and image cytometry as impossible to circumvent in complement to molecular biology so to avoid false interpretation on receptor expressions. Once confirmed by further large-scale studies, our preliminary results suggest a role for SSTR5 and CCR7 in CD pathogenesis.

journal_name

Mediators Inflamm

authors

Taquet N,Dumont S,Vonesch JL,Hentsch D,Reimund JM,Muller CD

doi

10.1155/2009/285812

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

285812

eissn

0962-9351

issn

1466-1861

journal_volume

2009

pub_type

杂志文章
  • Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1.

    abstract::Little work is done to develop Aloe vera (AV) using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacill...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2016/2945650

    authors: Jiang M,Deng K,Jiang C,Fu M,Guo C,Wang X,Wang X,Meng F,Yang S,Deng K,Chen T,Xin H

    更新日期:2016-01-01 00:00:00

  • Interleukin 35 Polymorphisms Are Associated with Decreased Risk of Premature Coronary Artery Disease, Metabolic Parameters, and IL-35 Levels: The Genetics of Atherosclerotic Disease (GEA) Study.

    abstract::Interleukin 35 (IL-35) is a heterodimeric cytokine involved in the development of atherosclerosis. The aim of the present study was to establish if the polymorphisms of IL-12A and EBI3 genes that encode the IL-35 subunits are associated with the development of premature coronary artery disease (CAD) in Mexican individ...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2017/6012795

    authors: Posadas-Sánchez R,Pérez-Hernández N,Angeles-Martínez J,López-Bautista F,Villarreal-Molina T,Rodríguez-Pérez JM,Fragoso JM,Posadas-Romero C,Vargas-Alarcón G

    更新日期:2017-01-01 00:00:00

  • High-Fat Diet Aggravates the Intestinal Barrier Injury via TLR4-RIP3 Pathway in a Rat Model of Severe Acute Pancreatitis.

    abstract:Objective:For patients with severe acute pancreatitis (SAP), a high body mass index (BMI) increases the possibility of infection derived from the intestine. In this study, we evaluate whether TAK242 can alleviate severe acute pancreatitis-associated injury of intestinal barrier in high-fat diet-fed rats. Methods:A SAP...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2019/2512687

    authors: Su YR,Hong YP,Mei FC,Wang CY,Li M,Zhou Y,Zhao KL,Yu J,Wang WX

    更新日期:2019-12-17 00:00:00

  • Circulating proangiogenic cytokines and angiogenesis inhibitor endostatin in untreated patients with chronic lymphocytic leukemia.

    abstract::The serum concentration of two pro-angiogenic cytokines: basic fibroblast growth factor (bFGF) and transforming growth factor beta1 (TGF-beta1), and anti-angiogenic factor endostatin in the serum of 80 never treated B-cell chronic lymphocytic leukemia (CLL) patients and 27 healthy volunteers was measured using an enzy...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1080/0962935031000134888

    authors: Gora-Tybor J,Blonski JZ,Robak T

    更新日期:2003-06-01 00:00:00

  • Reduction of IL-17A might suppress the Th1 response and promote the Th2 response by boosting the function of Treg cells during silica-induced inflammatory response in vitro.

    abstract::Silica inhalation can induce chronic lung inflammation and fibrosis. Upon silica stimulation, activated macrophages trigger the T-lymphocyte which can differentiate into many different types of Th cells, including the recently discovered Th17 cells. IL-17A, the typical Th17 cytokine, is reported in some inflammatory d...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2014/570894

    authors: Tang W,Liu F,Chen Y,Song L,Dai W,Li C,Weng D,Chen J

    更新日期:2014-01-01 00:00:00

  • Hemorrhagic Shock Sensitized the Diaphragm to Ventilator-Induced Dysfunction through the Activation of IL-6/JAK/STAT Signaling-Mediated Autophagy in Rats.

    abstract::Mechanical ventilation (MV) is a major life support technique for the management of trauma-associated hemorrhagic shock (HS). Ventilator-induced diaphragm dysfunction (VIDD), one of the most common complications of MV, has been well demonstrated in animal and human studies. However, few data are available concerning t...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2019/3738409

    authors: Zhang LJ,Ni SZ,Zhou XL,Zhao Y

    更新日期:2019-11-14 00:00:00

  • Neuroinflammation Induced by Surgery Does Not Impair the Reference Memory of Young Adult Mice.

    abstract::Postoperative cognitive dysfunction (POCD) increases morbidity and mortality after surgery. But the underlying mechanism is not clear yet. While age is now accepted as the top one risk factor for POCD, results from studies investigating postoperative cognitive functions in adults have been controversial, and data abou...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2016/3271579

    authors: Zhao Y,Huang L,Xu H,Wu G,Zhu M,Tian J,Wang H,Wang X,Yu W,Yang L,Su D

    更新日期:2016-01-01 00:00:00

  • Testosterone-induced effects on lipids and inflammation.

    abstract::Chronic pain has to be considered in all respects a debilitating disease and 10-20% of the world's adult population is affected by this disease. In the most general terms, pain is symptomatic of some form of dysfunction and (often) the resulting inflammatory processes in the body. In the study of pain, great attention...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2013/183041

    authors: Vodo S,Bechi N,Petroni A,Muscoli C,Aloisi AM

    更新日期:2013-01-01 00:00:00

  • Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis.

    abstract::The effects of intravenous injection of Porphyromonas gingivalis (Pg) on rabbit inflammatory immune response and atherosclerosis were evaluated by establishing a microamount Pg bacteremia model combined with high-fat diet. Twenty-four New Zealand rabbits were randomly divided into Groups A-D (n = 6). After 14 weeks, l...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2015/364391

    authors: Lin G,Chen S,Lei L,You X,Huang M,Luo L,Li Y,Zhao X,Yan F

    更新日期:2015-01-01 00:00:00

  • Dexmedetomidine Attenuates Monocyte-Endothelial Adherence via Inhibiting Connexin43 on Vascular Endothelial Cells.

    abstract::Current studies have identified the multifaceted protective functions of dexmedetomidine on multiple organs. For the first time, we clarify effects of dexmedetomidine on monocyte-endothelial adherence and whether its underlying mechanism is relative to connexin43 (Cx43), a key factor regulating monocyte-endothelial ad...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2020/7039854

    authors: Chai Y,Yu R,Liu Y,Wang S,Yuan D,Chen J

    更新日期:2020-02-10 00:00:00

  • COVID-19 and Toll-Like Receptor 4 (TLR4): SARS-CoV-2 May Bind and Activate TLR4 to Increase ACE2 Expression, Facilitating Entry and Causing Hyperinflammation.

    abstract::Causes of mortality from COVID-19 include respiratory failure, heart failure, and sepsis/multiorgan failure. TLR4 is an innate immune receptor on the cell surface that recognizes pathogen-associated molecular patterns (PAMPs) including viral proteins and triggers the production of type I interferons and proinflammator...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2021/8874339

    authors: Aboudounya MM,Heads RJ

    更新日期:2021-01-14 00:00:00

  • IL-10-Producing B Cells Suppress Effector T Cells Activation and Promote Regulatory T Cells in Crystalline Silica-Induced Inflammatory Response In Vitro.

    abstract::Long-term exposure to crystalline silica leads to silicosis, which is characterized by persistent lung inflammation and lung fibrosis. Multiple immune cells have been demonstrated to participate in crystalline silica-induced immune responses. Our previous study indicated that B10 could control lung inflammation throug...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2017/8415094

    authors: Lu Y,Liu F,Li C,Chen Y,Weng D,Chen J

    更新日期:2017-01-01 00:00:00

  • Plasma macrophage colony-stimulating factor levels during cardiopulmonary bypass with extracorporeal circulation.

    abstract::Leukocytosis and thrombocytopenia occur during cardiopulmonary bypass (CPB) with extracorporeal circulation (ECC). Elevated circulating concentrations of macrophage colony-stimulating factor (M-CSF) are reported during thrombocytopenia and leukopenia of different origins. We have assessed M-CSF concentrations in 40 pa...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/S0962935196000518

    authors: Denizot Y,Fixe P,Cornu E,Nathan N

    更新日期:1996-01-01 00:00:00

  • Lactoferrin levels in the gastric tissue of Helicobacter pylori-positive and -negative patients and its effect on anemia.

    abstract:AIM:To determine gastric tissue lactoferrin (Lf) levels of Helicobacter pylori- (Hp-) positive and -negative patients and its effect on anemia. METHODS:Cases in which initial presentation was of abdominal pain and that were Hp-positive at endoscopy were included. Hp-positive cases and -negative controls were divided i...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2012/214581

    authors: Doğan Y,Erkan T,Önal Z,Usta M,Doğusoy G,Çokuğraş FÇ,Kutlu T

    更新日期:2012-01-01 00:00:00

  • Protection from endotoxic uveitis by intravitreal Resolvin D1: involvement of lymphocytes, miRNAs, ubiquitin-proteasome, and M1/M2 macrophages.

    abstract::This study investigated the protective effects of intravitreal Resolvin D1 (RvD1) against LPS-induced rat endotoxic uveitis (EIU). RvD1 was administered into the right eye at a single injection of 5 μL volume containing 10-100-1000 ng/kg RvD1 1 h post-LPS injection (200 μg, Salmonella minnesota) into thefootpad of Spr...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2015/149381

    authors: Rossi S,Di Filippo C,Gesualdo C,Potenza N,Russo A,Trotta MC,Zippo MV,Maisto R,Ferraraccio F,Simonelli F,D'Amico M

    更新日期:2015-01-01 00:00:00

  • Effect of Blood Component Coatings of Enosseal Implants on Proliferation and Synthetic Activity of Human Osteoblasts and Cytokine Production of Peripheral Blood Mononuclear Cells.

    abstract::The study monitored in vitro early response of connective tissue cells and immunocompetent cells to enosseal implant materials coated by different blood components (serum, activated plasma, and plasma/platelets) to evaluate human osteoblast proliferation and synthetic activity and inflammatory response presented as a ...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2016/8769347

    authors: Himmlova L,Kubies D,Hulejova H,Bartova J,Riedel T,Stikarova J,Suttnar J,Pesakova V

    更新日期:2016-01-01 00:00:00

  • Temporomandibular joint bone tissue resorption in patients with early rheumatoid arthritis can be predicted by joint crepitus and plasma glutamate level.

    abstract::The aim was to investigate whether bone tissue resorption in early RA is related to crepitus of the temporomandibular joint (TMJ) and systemic levels of inflammatory mediators and markers and sex steroid hormones. Twentynine women and 18 men with recently diagnosed RA were examined for TMJ bone erosions with computeri...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2010/627803

    authors: Hajati AK,Näsström K,Alstergren P,Bratt J,Kopp S

    更新日期:2010-01-01 00:00:00

  • Dietary Magnesium Intake and Leukocyte Telomere Attrition in Adults: The Regulatory Role of Serum Tumor Necrosis Factor α.

    abstract:Objectives:In this study, we assessed the effects of dietary magnesium on leukocyte telomere length (LTL). Designs:The current cross-sectional analysis was based on data collected within a type 2 diabetes project. Settings. Dietary magnesium intake is associated with peripheral blood leukocyte telomere length (LTL). H...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2020/7610436

    authors: Yu J,Liu H,He S,Li P,Ma C,Ma M,Liu Y,Lv L,Ping F,Zhang H,Li W,Sun Q,Xu L,Li Y

    更新日期:2020-05-22 00:00:00

  • Inhaled corticosteroid effects both eosinophilic and non-eosinophilic inflammation in asthmatic patients.

    abstract:AIM:To determine induced sputum cell counts and interleukin-8 (IL-8), tumor necrosis factor alpha (TNF-alpha) and leukotriene B4 (LTB4) levels as markers of neutrophilic inflammation in moderate persistent asthma, and to evaluate the response to inhaled steroid therapy. METHODS:Forty-five moderate asthmatic patients a...

    journal_title:Mediators of inflammation

    pub_type: 临床试验,杂志文章

    doi:10.1080/09629350400003118

    authors: Basyigit I,Yildiz F,Ozkara SK,Boyaci H,Ilgazli A

    更新日期:2004-08-01 00:00:00

  • Tumour necrosis factor-alpha mediates blood-brain barrier damage in HIV-1 infection of the central nervous system.

    abstract::The pathogenesis of brain inflammation and damage by human immunodeficiency virus (HIV) infection is unclear. Because blood-brain barrier damage and impaired cerebral perfusion are common features of HIV-1 infection, we evaluated the role of tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) in ...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/S0962935192000292

    authors: Sharief MK,Ciardi M,Thompson EJ,Sorice F,Rossi F,Vullo V,Cirelli A

    更新日期:1992-01-01 00:00:00

  • Interactions between Gut Microbiota and Immunomodulatory Cells in Rheumatoid Arthritis.

    abstract::Rheumatoid arthritis (RA) is one of the most common autoimmune diseases caused by abnormal immune activation and immune tolerance. Immunomodulatory cells (ICs) play a critical role in the maintenance and homeostasis of normal immune function and in the pathogenesis of RA. The human gastrointestinal tract is inhabited ...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2020/1430605

    authors: Xu H,Zhao H,Fan D,Liu M,Cao J,Xia Y,Ju D,Xiao C,Guan Q

    更新日期:2020-09-09 00:00:00

  • Prevention of LPS-induced acute lung injury in mice by progranulin.

    abstract::The acute respiratory distress syndrome (ARDS), a clinical complication of severe acute lung injury (ALI) in humans, is a leading cause of morbidity and mortality in critically ill patients. Despite decades of research, few therapeutic strategies for clinical ARDS have emerged. Here we carefully evaluated the effect o...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2012/540794

    authors: Guo Z,Li Q,Han Y,Liang Y,Xu Z,Ren T

    更新日期:2012-01-01 00:00:00

  • Exposure to Air Pollution Exacerbates Inflammation in Rats with Preexisting COPD.

    abstract::Particulate matter with an aerodynamic diameter equal or less than 2.5 micrometers (PM2.5) is associated with the development of chronic obstructive pulmonary disease (COPD). The mechanisms by which PM2.5 accelerates disease progression in COPD are poorly understood. In this study, we aimed to investigate the effect o...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2020/4260204

    authors: Wang J,Li Y,Zhao P,Tian Y,Liu X,He H,Jia R,Oliver BG,Li J

    更新日期:2020-05-08 00:00:00

  • Chemokines and chemokine receptors in multiple sclerosis.

    abstract::Multiple sclerosis is an autoimmune disease with classical traits of demyelination, axonal damage, and neurodegeneration. The migration of autoimmune T cells and macrophages from blood to central nervous system as well as the destruction of blood brain barrier are thought to be the major processes in the development o...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2014/659206

    authors: Cheng W,Chen G

    更新日期:2014-01-01 00:00:00

  • Anti-inflammatory effects of ellagic acid on acute lung injury induced by acid in mice.

    abstract::Acute lung injury (ALI) is characterized by alveolar edema and uncontrolled neutrophil migration to the lung, and no specific therapy is still available. Ellagic acid, a compound present in several fruits and medicinal plants, has shown anti-inflammatory activity in several experimental disease models. We used the non...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2013/164202

    authors: Cornélio Favarin D,Martins Teixeira M,Lemos de Andrade E,de Freitas Alves C,Lazo Chica JE,Artério Sorgi C,Faccioli LH,Paula Rogerio A

    更新日期:2013-01-01 00:00:00

  • Urinary eosinophil protein X in children with atopic asthma.

    abstract:UNLABELLED:The aim of this study was to investigate the relationship between urinary eosinophil protein X (uEPX) and asthma symptoms, lung function, and other markers of eosinophilic airway inflammation in asthmatic school children. METHODS:A cross-sectional study was performed in 180 steroid dependent atopic children...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/2007/49240

    authors: Nuijsink M,Hop WC,Sterk PJ,Duiverman EJ,Hiemstra PS,de Jongste JC,CATO Study Group.

    更新日期:2007-01-01 00:00:00

  • The Th1:th2 dichotomy of pregnancy and preterm labour.

    abstract::Pregnancy is a unique immunological state in which a balance of immune tolerance and suppression is needed to protect the fetus without compromising the mother. It has long been established that a bias from the T helper 1 cytokine profile towards the T helper 2 profile contributes towards successful pregnancy maintena...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2012/967629

    authors: Sykes L,MacIntyre DA,Yap XJ,Teoh TG,Bennett PR

    更新日期:2012-01-01 00:00:00

  • Erratum to "Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines".

    abstract::[This corrects the article DOI: 10.1155/2018/3809092.]. ...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,已发布勘误

    doi:10.1155/2019/4524179

    authors: Curtiss E,Jiang Y,Liu L,Hawthorne C,Zhang J,Steinle JJ

    更新日期:2019-06-18 00:00:00

  • From Placenta to Polycystic Ovarian Syndrome: The Role of Adipokines.

    abstract::Adipokines are cytokines produced mainly by adipose tissue, besides many other tissues such as placenta, ovaries, peripheral-blood mononuclear cells, liver, muscle, kidney, heart, and bone marrow. Adipokines play a significant role in the metabolic syndrome and in cardiovascular diseases, have implications in regulati...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章,评审

    doi:10.1155/2016/4981916

    authors: Sartori C,Lazzeroni P,Merli S,Patianna VD,Viaroli F,Cirillo F,Amarri S,Street ME

    更新日期:2016-01-01 00:00:00

  • Tumour necrosis factor-alpha and adenosine in endotoxin shockleading related cardiovascular symptoms.

    abstract::We have observed uncontrollable cardiogenic shock as a cardiovascular manifestation of systemic inflammatory response syndrome (SIRS) leading to death in a 62-year-old woman. The diagnosis of SIRS was based on the demonstration of endotoxinaemia, and highly elevated plasma levels of tumour necrosis factor (TNF)-alpha,...

    journal_title:Mediators of inflammation

    pub_type: 杂志文章

    doi:10.1155/S0962935195000731

    authors: Sipka S,Seres T,Dinya Z,Szekanecz Z,Szentmiklósi J,Bodolay E,Szegedi G

    更新日期:1995-01-01 00:00:00