Abstract:
:MicroRNAs (miRNAs) are short, non-coding RNAs that negatively regulate target gene expression, and play an important role in cerebral ischemic injury. MiR-146a has been reported to be highly related to cell invasion, metastasis, immunity, inflammation and apoptosis. Previous studies have indicated that miR-146a can either inhibit or promote apoptosis through different pathophysiological processes. In our previous study, miR-146a in the blood was down-regulated during acute ischemic stroke. However, the connection between miR-146a and acute cerebral ischemic injury and the mechanism underlying the connection remain unclear. Here, we aimed to investigate the role of miR-146a and its possible target genes in human SK-N-SH cells subjected to 16h of oxygen-glucose deprivation and 12h of reperfusion (OGD/R) injury. Cells were transfected with miR-146a mimic or inhibitor to alter the expression of miR-146a. MiR-146a in the SK-N-SH cells was down-regulated after OGD/R injury. Moreover, bioinformatics analysis and dual luciferase assays demonstrated that miR-146a directly recognized the 3'-UTR of the pro-apoptotic genes, Caspase7 and Bcl-2-associated transcription factor 1 (Bclaf1). Furthermore, miR-146a over-expression effectively decreased the mRNA and protein expression of Caspase7 and Bclaf1, and aggravated OGD/R-induced cell apoptosis; in contrast, miR-146a down-regulation was neuroprotective. In conclusion, our study revealed that miR-146a contributes to OGD/R injury in vitro, while negatively regulating the pro-apoptotic genes, Caspase7 and Bclaf1. This special mechanism provides new insight into miRNA regulatory networks. In addition, miR-146a may offer a potential therapeutic approach to cerebral ischemic injury.
journal_name
Brain Resjournal_title
Brain researchauthors
Zhou X,Su S,Li S,Pang X,Chen C,Li J,Liu Jdoi
10.1016/j.brainres.2016.07.034subject
Has Abstractpub_date
2016-10-01 00:00:00pages
136-143issue
Pt Aeissn
0006-8993issn
1872-6240pii
S0006-8993(16)30516-9journal_volume
1648pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::The role of perceptual load in selective attention to multidimensional objects was investigated by independently manipulating the load along the task-relevant and the task-irrelevant dimensions in the central search array, which was flanked by congruent, incongruent, or neutral peripheral distractors. The relative bot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.07.071
更新日期:2006-10-09 00:00:00
abstract::In this study, we aimed at investigating the effect of an enriched environment (EE) on the recovery from chronic inflammatory pain. Inflammatory pain was induced by the injection of 2 mg of carrageenan (CAR) into the right knee of male Sprague-Dawley rats (n=34). Rats were housed either singly (S-housed) or in an EE (...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2009.04.039
更新日期:2009-06-18 00:00:00
abstract::Hypothalamic nuclei close to the third ventricle (VIII) represent key structures in avian osmoregulation concerned with the control of salt gland activity and release of the antidiuretic hormone [Arg8]vasotocin (AVT). Nitric oxide (NO) acting as a paracrine transmitter in the hypothalamus has been shown to contribute ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01205-6
更新日期:1999-04-17 00:00:00
abstract::To determine the contribution of presynaptic cholinergic mechanisms to the increased sensitivity of a genetically selected line of Sprague-Dawley rats (Flinders S-line) to the anticholinesterase, diisopropyl fluorophosphate (DFP), rats were sacrificed by focused microwave irradiation of the head 1 min after a pulse in...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91044-8
更新日期:1984-03-05 00:00:00
abstract::Two different ligands, tritiated oxytocin and a newly synthesized and monoiodinated oxytocin antagonist, were used to reveal sites which bind oxytocin in the brainstem and upper spinal cord of 12 human subjects. Tissue sections were incubated with either ligand at a concentration close to their respective dissociation...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90317-x
更新日期:1989-10-23 00:00:00
abstract::This study examined the projection from the rostral nucleus of the solitary tract (rNST) to the medial parabrachial nucleus (mPBN) in male Wistar rats using DiI as a retrograde tracer and biotinylated dextran as an anterograde tracer. Following successful unilateral injection of DiI into the mPBN (n = 8), retrogradely...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(96)00736-6
更新日期:1996-10-21 00:00:00
abstract::The actions of sodium valproate (NaVP) were studied in the in vitro hippocampus using extracellular, intracellular and voltage-clamp recording techniques. In the CA1 region, concentrations of 30-200 microM NaVP reduced the amplitude but not the time course of post-tetanic potentiation (PTP) of dendritic field excitato...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)90678-6
更新日期:1988-11-22 00:00:00
abstract::Extracellular recordings from 141 rat supraoptic nucleus neurons maintained in vitro in a perfused hypothalamic explant indicated that the excitability of 85% of cells was enhanced by the addition of 10-200 microM norepinephrine (NE) and the alpha-agonist methoxamine (MOXY), but not by isoproterenol, to the perfusion ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90499-2
更新日期:1984-07-30 00:00:00
abstract::Knife cuts in the frontal plane separating the anterior part of the caudate-putamen from the globus pallidus resulted in marked decreases in substances P levels in the reticular part of the substantia nigra. More caudal knife cuts were required in order to effect maximal decreases in nigral glutamic acid decarboxylase...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(77)91034-4
更新日期:1977-10-28 00:00:00
abstract::The locus coeruleus was stimulated in 62 cats in order to investigate the effect on cephalic blood flow and cephalic vascular resistance. Flow was measured by electromagnetic flow probes applied to the common carotid artery. Stimulation over a range of frequencies (0.2-200 s-1) produced a frequency-dependent fall in c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90236-6
更新日期:1983-11-14 00:00:00
abstract::The possibility that the motor cortex receives peripheral input directly from the thalamus was examined using the evoked potential method and the following results were obtained. Potentials in the motor cortex evoked by stimulation of superficial radial (SR) or group II deep radial (DR) nerve were neither abolished no...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90532-8
更新日期:1979-08-24 00:00:00
abstract::The changes in mean arterial pressure (MAP) and respiratory frequency (RF) in response to microinjection of NMDA or AMPA into the commissural subnucleus of the NTS (comNTS) at the calamus scriptorius level of awake rats were evaluated. Under tribromoethanol anesthesia, the rats received guide-cannulae in direction of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.09.017
更新日期:2005-11-23 00:00:00
abstract::Glutamate kills sensitive neurons through several steps downstream to receptor activation: increased free Ca2+ levels, activation of various enzymes and accumulation of reactive oxygen species (ROS). We have evaluated in a well established model of neuronal cultures the neuroprotective effects of blocking these mechan...
journal_title:Brain research
pub_type: 杂志文章
doi:
更新日期:1996-07-22 00:00:00
abstract::Auditory stimulation applied during rapid eye movement (REM) sleep enhances the duration of REM sleep in cats and humans. The present experiment investigated whether auditory stimulation would enhance REM sleep in young (3-6 months) rats, and also in old (22-24 months) rats which have impaired REM sleep. Baseline slee...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91299-t
更新日期:1992-09-04 00:00:00
abstract::At a physiologically relevant concentration (1.25 microM), aluminum had an activating effect on oligodendrocyte cell cultures, similar to that previously reported for other cell types. G protein-linked signal transduction was stimulated as indicated by enhanced production of IP3, and protein synthesis was increased. A...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)02597-0
更新日期:2002-06-21 00:00:00
abstract::Acute and repeated exposure to cocaine induces long-lasting alterations in neural networks that underlie compulsive drug seeking and taking. Cocaine exposure triggers complex adaptations in the brain that are mediated by dynamic patterns of gene expression that are translated into enduring changes. Recently, epigeneti...
journal_title:Brain research
pub_type: 杂志文章,评审
doi:10.1016/j.brainres.2014.09.069
更新日期:2015-12-02 00:00:00
abstract::Calcium accumulation and neuronal injury of rat cortical cell cultures in vitro were examined following oxygen deprivation under conditions of normal and low glucose. 45Ca2+ uptake and lactate dehydrogenase (LDH) release, measured at 12 and 24 h after oxygen deprivation, were significantly elevated in cultures exposed...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91527-y
更新日期:1993-04-02 00:00:00
abstract::Dorsal temporal pole (dTP) is a cortical region at the rostral end of the superior temporal gyrus that forms part of the ventral auditory object processing pathway. Anatomical connections with frontal and medial temporal areas, as well as a recent single-unit recording study, suggest this area may be an important part...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.12.025
更新日期:2016-06-01 00:00:00
abstract::Oxidative stress and massive production of nitric oxide (NO) have been implicated in the neuropathogenesis following peripheral nerve injury. This study was aimed to ascertain whether melatonin would exert its neuroprotective effect on the lesioned hypoglossal neurons after peripheral axotomy, since it is known to red...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02489-6
更新日期:2000-08-11 00:00:00
abstract::Facial allodynia is a migraine symptom that is generally considered to represent a pivotal point in migraine progression. Treatment before development of facial allodynia tends to be more successful than treatment afterwards. As such, understanding the underlying mechanisms of facial allodynia may lead to a better und...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2017.03.011
更新日期:2017-06-01 00:00:00
abstract::Injection of inositol 1,4,5-trisphosphate (InsP3) into dark-adapted Limulus ventral photoreceptors produces a series of discrete bursts of membrane depolarization. Prior injection of aequorin, a luminescent calcium indicator, reveals that the bursts of depolarization are accompanied by individual bursts of intracellul...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90860-2
更新日期:1987-10-13 00:00:00
abstract::Murine spinal cord primary mixed cultures were treated with the respiratory inhibitor, rotenone, to mimic hypoxic conditions. Under these conditions neurons rapidly underwent oncosis (necrosis) with a complete loss in viability occurring within 260 min; however, astrocytes, which accounted for most of the cell populat...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)01086-0
更新日期:1999-03-13 00:00:00
abstract::An investigation of reflex responses of leg muscles to sural stimulation during stepping was performed on human subjects. Non-noxious electrical stimulation applied during the swing phase or the latter half of the stance phase produced a mixed increase and decrease of EMG activity in the hamstring muscles. No response...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90123-3
更新日期:1983-12-12 00:00:00
abstract::Thalamocortical connections were studied after injections of retrograde tracers were made into rostral or caudal parts of cortical area Fr2 in adult rats. The data reveal that both rostral Fr2 (rFr2) and caudal Fr2 (cFr2) receive input from the centrolateral, central medial, interanteromedial, mediodorsal, paracentral...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91420-6
更新日期:1991-12-24 00:00:00
abstract::Many of our everyday actions are only appropriate in certain situations and selecting the appropriate behavior requires that we use current context and previous experience to guide our decisions. The current study examined hippocampal functional connectivity with prefrontal and striatal regions during a task that requ...
journal_title:Brain research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.brainres.2011.09.038
更新日期:2011-11-14 00:00:00
abstract::In the sea hare, Aplysia californica, consummatory feeding behavior is selectively abolished by bilateral crushes of the cerebral-buccal connectives and recovers by postlesion day 13. Recovered biting responses are initially weak and increase in magnitude gradually with time. The lesions do not affect appetitive feedi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91217-3
更新日期:1992-07-10 00:00:00
abstract::The effect of intraocular injection of the neurotoxin, kainic acid, on the retinofugal pathway has been examined by anterograde transport of horseradish peroxidase. Although less peroxidase was present in all the primary optic centers after the intraocular injection of kainic acid than in the controls, the area occupi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90939-9
更新日期:1984-01-09 00:00:00
abstract::We observed chronological changes of platelet endothelial cell adhesion molecule-1 (PECAM-1), final mediator of neutrophil transendothelial migration, immunoreactivity, and protein level in the gerbil hippocampus proper after 5 min of transient ischemia. One day after ischemic insult, PECAM-1 immunoreactivity and prot...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.04.049
更新日期:2005-06-28 00:00:00
abstract::The interpeduncular nucleus (IPN) was stimulated electrically while single-cell activity was recorded in the nucleus raphe magnus (NRM) of rats under pentobarbital anesthesia. Two classes of NRM cell were examined, those inhibited (off-cells) and those excited (on-cells) by noxious mechanical skin stimulation. Off-cel...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(90)91476-w
更新日期:1990-07-09 00:00:00
abstract::Treatment with thyrotropin-releasing hormone (TRH) significantly improved survival following induction of fatal systemic anaphylaxis in mice. The protective effect was mediated centrally since survival was increased by intracerebroventricular (i.c.v.) administration of TRH at doses which had no effect when given syste...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)91421-5
更新日期:1984-04-30 00:00:00