Abstract：
:Medical studies have consistently shown that the best defense against cancer is early detection. Due to this, many efforts have been made to develop methods of screening patient blood quickly and cheaply. These methods range from separation via differences in size, electrostatic potential, chemical potential, antibody-binding affinity, among others. We propose a method of separating cells which have similar size and outer coatings, but which differ in their elastic properties. Such a method would be useful in detecting cancerous cells, which may have similar properties to leukocytes or erythrocytes but differ in their stiffness and deformation response. Here, we use coarse-grained model of a cell with membrane, cytoskeleton, and inner fluid to determine how small changes in the cell stiffness may be used to quickly and efficiently separate out irregular cells such as circulating tumor cells from a sample of blood. We focus specifically on the effects of volumetric flux and plate geometry on the ability of a separation plate to differentiate cells of similar but disparate stiffnesses. We show that volumetric flux is crucial in determining the stiffness cutoff for separating out cells of similar sizes, while the angle of the separation plate plays a less important role. With this work, we provide a comprehensive approach to the design factors of cell separation via elastic properties and hope to offer a guideline for the development of novel cytometry devices for the detection of irregular cells such as circulating tumor cells.
journal_name
Biomech Model Mechanobioljournal_title
Biomechanics and modeling in mechanobiologyauthors
Becton M,Averett RD,Wang Xdoi
10.1007/s10237-018-1093-9subject
Has Abstractpub_date
2019-04-01 00:00:00pages
425-433issue
2eissn
1617-7959issn
1617-7940pii
10.1007/s10237-018-1093-9journal_volume
18pub_type
杂志文章abstract：:In this manuscript, we present a combined experimental and computational technique that can identify the heterogeneous elastic properties of planar soft tissues. By combining inverse membrane analysis, digital image correlation, and bulge inflation tests, we are able to identify a tissue's mechanical properties locall...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-014-0646-9
更新日期：2015-10-01 00:00:00
abstract：:Elastin and collagen fibers play important roles in the mechanical properties of aortic media. Because knowledge of local fiber structures is required for detailed analysis of blood vessel wall mechanics, we investigated 3D microstructures of elastin and collagen fibers in thoracic aortas and monitored changes during ...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-016-0851-9
更新日期：2017-06-01 00:00:00
abstract：:In this study, we hypothesized that spatial relationships exist between the local mechanical environment and inflammatory marker expression in atherosclerotic plaques, and that these relationships are plaque-progression dependent. Histologic cross-sections were collected at regular intervals along the length of diseas...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-009-0151-8
更新日期：2009-12-01 00:00:00
abstract：:By following the common definition of forward-dynamics simulations, i.e. predicting movement based on (neural) muscle activity, this work describes, for the first time, a forward-dynamics simulation framework of a musculoskeletal system, in which all components are represented as continuous, three-dimensional, volumet...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-016-0850-x
更新日期：2017-06-01 00:00:00
abstract：:A dynamic model is proposed for shear stress induced adenosine triphosphate (ATP) release from endothelial cells (ECs). The dynamic behavior of the ATP/ADP concentration at the endothelial surface by viscous shear flow is investigated through simulation studies based on the dynamic ATP release model. The numerical res...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-007-0088-8
更新日期：2008-10-01 00:00:00
abstract：:The level of hematocrit (Hct) is known to affect mean arterial pressure (MAP) by influencing blood viscosity. In the healthy population, an increase in Hct (and corresponding increase in viscosity) tends to raise MAP. However, data from a clinical study of type 2 diabetic patients indicate that this relationship is no...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0258-y
更新日期：2011-07-01 00:00:00
abstract：:It has been demonstrated that interstitial fluid (IF) flow can play a crucial role in tumor cell progression. Swartz and collaborators (Cancer Cell 11: 526-538, Shields et al. 2007) demonstrated that cells that secrete the lymphoid homing chemokines CCL21/CCL19 and express their receptor CCR7 could use flow to bias th...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-019-01128-2
更新日期：2019-08-01 00:00:00
abstract：:Cardiovascular implantable devices alter the biofluid dynamics and biochemistry of the blood in which they are placed. These perturbations can lead to thrombus formation which may or may not be desired, depending on the application. In this work, a computational model is developed that couples biofluid dynamics and bi...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-1059-y
更新日期：2018-12-01 00:00:00
abstract：:Both the clinically established diameter criterion and novel approaches of computational finite element (FE) analyses for rupture risk stratification of abdominal aortic aneurysms (AAA) are based on assumptions of population-averaged, uniform material properties for the AAA wall. The presence of inter-patient and intr...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-012-0436-1
更新日期：2013-08-01 00:00:00
abstract：:The primary capillary plexus in early yolk sacs is remodeled into matured vitelline vessels aligned in the direction of blood flow at the onset of cardiac contraction. We hypothesized that the influence of fluid shear stress on cellular behaviors may be an underlying mechanism by which some existing capillary channels...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-005-0001-2
更新日期：2005-12-01 00:00:00
abstract：:Adherent cells normally apply forces as a generic means of sensing and responding to the mechanical nature of their surrounding environment. How these forces vary as a function of the extracellular rigidity is critical to understanding the regulatory functions that drive important phenomena such as wound healing or mu...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-013-0508-x
更新日期：2014-04-01 00:00:00
abstract：:One of only a few approved and available anabolic treatments for severe osteoporosis is daily injections of PTH (1-34). This drug has a specific dual action which can act either anabolically or catabolically depending on the type of administration, i.e. intermittent or continuous, respectively. In this paper, we prese...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-020-01307-6
更新日期：2020-10-01 00:00:00
abstract：:Inflammation, a precursor to many diseases including cancer and atherosclerosis, induces differential surface expression of specific vascular molecules. Blood-borne nanoparticles (NPs), loaded with therapeutic and imaging agents, can recognize and use these molecules as vascular docking sites. Here, a computational mo...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-013-0520-1
更新日期：2014-06-01 00:00:00
abstract：:Hemolysis is a major concern in blood-circulating devices, which arises due to hydrodynamic loading on red blood cells from ambient flow environment. Hemolysis estimation models have often been used to aid hemocompatibility design. The preponderance of hemolysis models was formulated on the basis of laminar flows. How...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-01108-y
更新日期：2019-06-01 00:00:00
abstract：:Recently, physiological and biomechanical studies on animal models with metal implants filling full-thickness cartilage defects have resulted in good clinical outcomes. The knowledge of the time-dependent macroscopic behavior of cartilage surrounding the metal implant is essential for understanding the joint function ...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-011-0346-7
更新日期：2012-05-01 00:00:00
abstract：:The present study aims at investigating biomechanical failure behaviour of human aneurismal aortic tissues so as to diagnose the rupture risk of aneurysms more accurately. An inflation test is performed on aneurismal aortic tissues up to failure and full-field measurements are achieved using stereo digital image corre...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-011-0356-5
更新日期：2012-07-01 00:00:00
abstract：:In myocardial infarction, muscle tissue of the heart is damaged as a result of ceased or severely impaired blood flow. Survivors have an increased risk of further complications, possibly leading to heart failure. Material properties play an important role in determining post-infarction outcome. Due to spatial variatio...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-1028-5
更新日期：2018-10-01 00:00:00
abstract：:Collagen degradation is one of the early signs of osteoarthritis. It is not known how collagen degradation affects chondrocyte volume and morphology. Thus, the aim of this study was to investigate the effect of enzymatically induced collagen degradation on cell volume and shape changes in articular cartilage after a h...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-012-0409-4
更新日期：2013-06-01 00:00:00
abstract：:The controlled cortical impact (CCI) model is widely used in many laboratories to study traumatic brain injury (TBI). Although external impact parameters during CCI tests could be clearly defined, little is known about the internal tissue-level mechanical responses of the rat brain. Furthermore, the external impact pa...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0212-z
更新日期：2010-12-01 00:00:00
abstract：:The two main load bearing tissues of the intervertebral disc are the nucleus pulposus and the annulus fibrosus. Both tissues are composed of the same basic components, but differ in their organization and relative amounts. With degeneration, the clear distinction between the two tissues disappears. The changes in bioc...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-016-0835-9
更新日期：2017-04-01 00:00:00
abstract：:Nanomechanics of individual collagen fibrils govern the mechanical behavior of the majority of connective tissues, yet the current models lack significant details. Majority of the current models assume a rod-shape molecule with homogenous mechanical properties. Recent X-ray crystallography revealed significantly diffe...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-014-0615-3
更新日期：2015-06-01 00:00:00
abstract：:Computational models of the heart have reached a level of maturity that enables sophisticated patient-specific simulations and hold potential for important applications in diagnosis and therapy planning. However, such clinical use puts strict demands on the reliability and accuracy of the models and requires the sensi...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-019-01153-1
更新日期：2019-10-01 00:00:00
abstract：:Long-term adaptation of soft tissues is realized through growth and remodeling (G&R). Mathematical models are powerful tools in testing hypotheses on G&R and supporting the design and interpretation of experiments. Most theoretical G&R studies concentrate on description of either growth or remodeling. Our model combin...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0204-z
更新日期：2010-12-01 00:00:00
abstract：:Articular cartilage is a multicomponent, poroviscoelastic tissue with nonlinear mechanical properties vital to its function. A consequent goal of repair or replacement of injured cartilage is to achieve mechanical properties in the repair tissue similar to healthy native cartilage. Since fresh healthy human articular ...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-011-0338-7
更新日期：2012-05-01 00:00:00
abstract：:This paper evaluates numerically coupled blood flow and wall structure interactions in a representative stented abdominal aortic aneurysm (AAA) model, leading potentially to endovascular graft (EVG) failure. A total of 12 biomechanical contributors to possible EVG migration were considered. The results show that after...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-005-0003-0
更新日期：2005-12-01 00:00:00
abstract：:Computational fluid dynamics (CFD) is an increasingly used method for investigation of hemodynamic parameters and their alterations under pathological conditions, which are important indicators for diagnosis of cardiovascular disease. In hemodynamic simulation models, the employment of appropriate boundary conditions ...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-1045-4
更新日期：2018-12-01 00:00:00
abstract：:The key parameters determining the elastic properties of an unidirectional mineralized bone fibril-array decomposed in two further hierarchical levels are investigated using mean field methods. Modeling of the elastic properties of mineralized micro- and nanostructures requires accurate information about the underlyin...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-010-0190-1
更新日期：2010-10-01 00:00:00
abstract：:Locust wings are able to sustain millions of cycles of mechanical loading during the lifetime of the insect. Previous studies have shown that cross veins play an important role in delaying crack propagation in the wings. Do cross veins thus also influence the fatigue behaviour of the wings? Since many important fatigu...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-017-0930-6
更新日期：2017-12-01 00:00:00
abstract：:Skin expansion delivers newly grown skin that maintains histological and mechanical features of the original tissue. Although it is the gold standard for cutaneous defect correction today, the underlying mechanisms remain poorly understood. Here we present a novel technique to quantify anisotropic prestrain, deformati...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-015-0650-8
更新日期：2015-10-01 00:00:00
abstract：:Intracellular calcium dynamics plays an important role in the regulation of vascular endothelial cellular functions. In order to probe the intracellular calcium dynamic response under synergistic effect of wall shear stress (WSS) and adenosine triphosphate (ATP) signals, a novel microfluidic device, which provides the...
journal_title：Biomechanics and modeling in mechanobiology
pub_type： 杂志文章
doi：10.1007/s10237-018-1076-x
更新日期：2019-02-01 00:00:00
