Abstract：
:The bioinspired, microscale tissue engineering approach has emerged in recent years as a promising alternative to preformed scaffolds. Using this approach, complex tissues and organs can be efficiently engineered from microscale modules to replicate the intricate architecture and physiology of vascularized organs and tissues. Previously, we demonstrated assembly of a prototype, engineered liver tissue, formed by the fusion of hepatocyte-containing capsules. Here, we analyzed the effects of various controllable system parameters with the aim of predicting the operating limits of our modular tissue in high cell density, perfused cultures. Both the capsule diameter and construct height were limited by mass transfer requirements, while the shear stress on the capsule wall and the pressure drop across the packed capsule bed were dictated by the capsule diameter and permissible flow rates of the system. Our analysis predicts that capsules with a 200 µm radius can efficiently maintain hepatocytes at cell densities comparable to liver tissue. Some model predictions were validated by packed bed perfusion cultures. Flow-induced bed compaction hysteresis was tested experimentally and found to have minimal effect on flow characteristics. The effectiveness factor (η) for the overall oxygen transfer within packed beds of capsule modules was estimated to be 0.72 for all conditions. Primary hepatocytes encapsulated in the capsules exhibited normal metabolism and formed spheroids during a 7-day culture. The model predictions can be useful to study mass transfer and shear stress in high-density perfusion cultures. Overall, analysis of a perfused, capsule-based, modular tissue demonstrated the feasibility of the technology as a platform for fabrication of highly metabolic solid organs.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Annamalai RT,Matthew HWTdoi
10.1007/s10439-018-02192-ysubject
Has Abstractpub_date
2019-05-01 00:00:00pages
1223-1236issue
5eissn
0090-6964issn
1573-9686pii
10.1007/s10439-018-02192-yjournal_volume
47pub_type
杂志文章abstract：:Premature ovarian insufficiency (POI) is a major complication of cytotoxic treatments due to extreme ovarian sensitivity to chemotherapy and radiation. In pediatric cancer patients modern therapy has improved the long-term survival to over 80% in the United States. However, these cancer survivors face long-term health...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1780-6
更新日期：2017-07-01 00:00:00
abstract：:Linear autoregressive (AR) model-based heart rate (HR) spectral analysis has been widely used to study HR dynamics. Owing to system and measurement noise, the parameters of an AR model have intrinsic statistical uncertainty. In this study, we evaluate how this AR parameter uncertainty can translate to uncertainty in H...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368320
更新日期：1995-03-01 00:00:00
abstract：:To better understand oxygen utilization by the retina, a mathematical model of oxygen diffusion and consumption in the cat outer, avascular retina was developed by analyzing previously recorded profiles of oxygen tension (PO2) as a function of retinal depth. Simple diffusion modelling of the oxygen distribution throug...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368415
更新日期：1990-01-01 00:00:00
abstract：:Examining students' learning styles can yield information useful to the design of learning activities, courses, and curricula. A variety of measures have been used to characterize learning styles, but the literature contains little information specific to biomedical engineering (BMEN) students. We, therefore, utilized...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1512677
更新日期：2002-09-01 00:00:00
abstract：:A study that customizes the feature generator parameters of an asynchronous Brain Interface (BI) is discussed. The goal is to detect the presence of a certain pattern, in the ongoing EEG, associated with a specific movement and to improve the system's performance. Results of this study show that customization mostly b...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9097-5
更新日期：2006-06-01 00:00:00
abstract：:Poor prosthetic fit is often the result of heterotopic ossification (HO), a frequent problem following blast injuries for returning service members. Osseointegration technology offers an advantage for individuals with significant HO and poor socket tolerance by using direct skeletal attachment of a prosthesis to the d...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0050-2
更新日期：2010-09-01 00:00:00
abstract：:Typical inverse dynamics approaches to the calculation of muscle, ligament, and joint contact forces are based on a step-wise solution of the equations of motion. This approach is therefore limited in its ability to provide insight as to the muscular, ligamentous, and articular interactions that create joint stability...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0303-8
更新日期：2011-07-01 00:00:00
abstract：:This study was aimed at developing a physical model, supported by experimental observations, to describe the formation and growth of microbubbles seen in patients with mitral mechanical heart valves (MHV). This phenomenon, often referred to as high intensity transient signals (HITS), appears as bright, intense, high-v...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.231
更新日期：1999-11-01 00:00:00
abstract：:Development of increasingly complex integrated cellular systems will be a major challenge for the next decade and beyond, as we apply the knowledge gained from the sub-disciplines of regenerative medicine, synthetic biology, micro-fabrication and nanotechnology, systems biology, and developmental biology. In this pros...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-013-0902-7
更新日期：2014-02-01 00:00:00
abstract：:Polymeric heart valves have the potential to reduce thrombogenic complications associated with current mechanical valves and overcome fatigue-related problems experienced by bioprosthetic valves. In this paper we characterize the in vitro velocity and Reynolds Shear Stress (RSS) fields inside and downstream of three d...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9117-5
更新日期：2006-06-01 00:00:00
abstract：:We describe a system for real-time control of biological and other experiments. This device, based around the Real-Time Linux operating system, was tested specifically in the context of dynamic clamping, a demanding real-time task in which a computational system mimics the effects of nonlinear membrane conductances in...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1408929
更新日期：2001-10-01 00:00:00
abstract：:A major benefit of computational modeling in biomechanics research is its ability to estimate internal muscular demands given limited input information. However, several assumptions regarding model parameters and constraints may influence model outputs. This research evaluated the influence of model parameter variabil...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1035-3
更新日期：2014-09-01 00:00:00
abstract：:The efforts to develop structural materials for biodegradable metal implants have lately shifted their focus from Magnesium and Iron base alloys towards Zinc. This was mainly due to the accelerated corrosion rate of Mg that is accompanied by hydrogen gas evolution, formation of voluminous iron oxide products with redu...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02245-w
更新日期：2019-06-01 00:00:00
abstract：:We propose a multiphysical mathematical model by fully coupling lipid deposition, monocytes/macrophages recruitment and angiogenesis to investigate the pathophysiological responses of an atherosclerotic plaque to the dynamic changes in the microenvironment. The time evolutions of cellular (endothelial cells, macrophag...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02173-1
更新日期：2019-02-01 00:00:00
abstract：:Spine musculoskeletal models used to estimate loads and displacements require many simplifying assumptions. We examined how assumptions about subject size and vertebral positions can affect the model outcomes. Head and neck models were developed to represent 30 subjects (15 males and 15 females) in neutral posture and...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-2084-9
更新日期：2018-11-01 00:00:00
abstract：:We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1591189
更新日期：2003-09-01 00:00:00
abstract：:Thorax injuries mainly due to rib fractures have been associated with high rates of morbidity and mortality in motor vehicle crashes. Thoracic biomechanics has been studied extensively, but there are no robust biomechanical response targets for ribs that consider age, sex, body size, and vulnerability factors. The obj...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02613-x
更新日期：2021-02-01 00:00:00
abstract：:Wall stress analysis of abdominal aortic aneurysm (AAA) is a promising method of identifying AAAs at high risk of rupture. However, neglecting residual strains (RS) in the load-free configuration of patient-specific finite element analysis models is a sever limitation that strongly affects the computed wall stresses. ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0749-y
更新日期：2013-07-01 00:00:00
abstract：:Alzheimer's disease (AD) is an irreversible brain disorder which represents the most common form of dementia in western countries. An early and accurate diagnosis of AD would enable to develop new strategies for managing the disease; however, nowadays there is no single test that can accurately predict the development...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0155-7
更新日期：2011-01-01 00:00:00
abstract：:Although medical specialties have recognized the importance of using ultrasonic imaging, dentistry is only beginning to discover its benefit. This has particularly been important in the field of periodontics which studies infections in the gum and bone tissues that surround the teeth. This study investigates the feasi...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0089-0
更新日期：2010-11-01 00:00:00
abstract：:We have developed a method for quantifying the complexity of activation patterns observed during ventricular fibrillation (VF) that is based on our previously reported methodology for decomposing epicardial mapping data into a set of isolated wavefronts. One-half second datasets are acquired from a 21 x 24 array of un...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02684160
更新日期：1997-09-01 00:00:00
abstract：:Arterial restenosis following stent deployment may be influenced by the local flow environment within and around the stent. We have used computational fluid dynamics to investigate the flow field in the vicinity of model stents positioned within straight and curved vessels. Our simulations have revealed the presence o...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-2499-y
更新日期：2005-04-01 00:00:00
abstract：:This article presents a computer-aided detection system for capsule endoscopy (CE) images using contourlet-based color textural features to recognize tumors in the digestive tract. As tumor exhibits rich information in color texture, a novel color texture feature based on contourlet transform is proposed to describe c...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-011-0380-8
更新日期：2011-12-01 00:00:00
abstract：:Collagen is known to be a viscoelastic material and therefore is an important source of time dependent behavior in soft tissue. Though there are material models for soft tissue that rely on properties and structure of constituent collagen fibers, such models generally utilize only the elastic properties of collagen, a...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-006-9124-6
更新日期：2006-07-01 00:00:00
abstract：:We present here the first quantitative correlation for cell contact guidance in an oriented fibrillar network in terms of biased cell migration. The correlation is between the anisotropic cell diffusion parameter, DA = Dx/Dy, and the collagen gel birefringence, delta n, a measure of axially biased collagen fibril orie...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368241
更新日期：1994-07-01 00:00:00
abstract：:Blood flow simulations are making their way into the clinic, and much attention is given to estimation of fractional flow reserve in coronary arteries. Intracardiac blood flow simulations also show promising results, and here the flow field is expected to depend on the pulmonary venous (PV) flow rates. In the absence ...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-018-02153-5
更新日期：2019-02-01 00:00:00
abstract：:A role for mechanical stimulation in the control of cell fate has been proposed and mechanical conditioning of mesenchymal stem cells (MSCs) is of interest in directing MSC behavior for tissue engineering applications. This study investigates strain-induced differentiation and proliferation of MSCs, and investigates t...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9979-4
更新日期：2010-05-01 00:00:00
abstract：:Vibration under shear flow causes the reduction of flow resistance for shear-thinning fluids. The present study investigates the effect of vibration on the flow resistance of a nonaggregating red blood cell (RBC) suspension with a newly designed pressure-scanning capillary viscometer (PSCV). The PSCV was originally de...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1603751
更新日期：2003-10-01 00:00:00
abstract：:Presented is a discussion of steps towards the creation of a database of the microcirculation encompassing anatomical and functional experimental data, and conceptual and computational models. The discussion includes issues of database utility, organization, data deposition, and linkage to other databases. The databas...
journal_title：Annals of biomedical engineering
pub_type：
doi：10.1114/1.112
更新日期：1998-11-01 00:00:00
abstract：:The false vocal folds are believed to be components of the acoustic filter that is responsible for shaping the voice. However, the effects of false vocal folds on the vocal fold vibration and the glottal aerodynamic during phonation remain unclear. This effect has implications for computational modeling of phonation a...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-008-9630-9
更新日期：2009-03-01 00:00:00
