Abstract：
:A role for mechanical stimulation in the control of cell fate has been proposed and mechanical conditioning of mesenchymal stem cells (MSCs) is of interest in directing MSC behavior for tissue engineering applications. This study investigates strain-induced differentiation and proliferation of MSCs, and investigates the cellular mechanisms of mechanotransduction. MSCs were seeded onto a collagen-coated silicone substrate and exposed to cyclic tensile mechanical strain of 2.5% at 0.17 Hz for 1-14 days. To examine mechanotransduction, cells were strained in the presence of the stretch-activated cation channel (SACC) blocker, gadolinium chloride (GdCl(3)); the extracellular regulated kinase (ERK) inhibitor, U0126; the p38 inhibitor, SB203580; and the phosphatidylinosito1 3-kinase (PI3-kinase) inhibitor, LY294002. Following exposure to strain, the osteogenic markers Cbfalpha1, collagen type I, osteocalcin, and BMP2 were temporally expressed. Exposure to strain in the presence of GdCl(3) (10 microM) reduced the induction of collagen I expression, thus identifying a role for SACC, at least in part, as mechanosensors in strain-induced MSC differentiation. The strain-induced synthesis of BMP2 was found to be reduced by inhibitors of the kinases, ERK, p38, and PI3 kinase. Additionally, mechanical strain reduced the rate of MSC proliferation. The identification of the mechanical control of MSC proliferation and the molecular link between mechanical stimulation and osteogenic differentiation has consequences for regenerative medicine through the development of a functional tissue engineering approach.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Kearney EM,Farrell E,Prendergast PJ,Campbell VAdoi
10.1007/s10439-010-9979-4subject
Has Abstractpub_date
2010-05-01 00:00:00pages
1767-79issue
5eissn
0090-6964issn
1573-9686journal_volume
38pub_type
杂志文章abstract：:Arterial restenosis following stent deployment may be influenced by the local flow environment within and around the stent. We have used computational fluid dynamics to investigate the flow field in the vicinity of model stents positioned within straight and curved vessels. Our simulations have revealed the presence o...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-2499-y
更新日期：2005-04-01 00:00:00
abstract：BACKGROUND:This work explores for the first time the effects of temperature increments on the development of high shear stresses between plaque and arterial wall due to their different dilatational properties. Data from the literature report febrile reactions prior to myocardial infarction in patients with normal coron...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9397-4
更新日期：2008-01-01 00:00:00
abstract：:The design and operating characteristics of the first self-contained in-the-ear device to deliver altered auditory feedback is described for applications with those who stutter. The device incorporates a microdigital signal processor core that reproduces the high fidelity of unaided listening and auditory self-monitor...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1541014
更新日期：2003-02-01 00:00:00
abstract：:The cerebral blood flow (CBF) response to changes in perfusion pressure mediated through decreases in arterial pressure, increases in cerebrospinal fluid (CSF) pressure and increases in jugular venous pressure was studied in anesthetized dogs. A preparation was developed in which each of the three relevant pressures c...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584249
更新日期：1985-01-01 00:00:00
abstract：:The aim of this study was to test the differentiative effects of osteoblasts after treatment with a static magnetic field (SMF). MG63 osteoblast-like cells were exposed to a 0.4-T SMF. The differentiation markers were assessed by observing the changes in alkaline phosphatase activity and electron microscopy images. Me...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9370-2
更新日期：2007-11-01 00:00:00
abstract：:In order to increase the probability of having a successful cataract post-surgery, the customisation of the haptic design of the intraocular lens (IOL) according to the characteristics of the patient is recommended. In this study, we present two prediction models based on deep neural networks (DNNs). One is capable of...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02636-4
更新日期：2020-12-01 00:00:00
abstract：:Linear autoregressive (AR) model-based heart rate (HR) spectral analysis has been widely used to study HR dynamics. Owing to system and measurement noise, the parameters of an AR model have intrinsic statistical uncertainty. In this study, we evaluate how this AR parameter uncertainty can translate to uncertainty in H...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368320
更新日期：1995-03-01 00:00:00
abstract：:Measurement of cell shortening is an important technique for assessment of physiology and pathophysiology of cardiac myocytes. Many types of heart disease are associated with decreased myocyte shortening, which is commonly caused by structural and functional remodeling. Here, we present a new approach for local measur...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1593-7
更新日期：2016-10-01 00:00:00
abstract：:The proliferation, migration, and adhesion of vascular smooth muscle cells (VSMCs) and their interactions with extracellular matrix are key features of atherosclerosis and restenosis. Recently, there has been evidence that magnetic fields exert multiple effects on the biological performance of cells and may aid in the...
journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
doi：10.1007/s10439-012-0602-8
更新日期：2012-12-01 00:00:00
abstract：:To study pulsatile pressure-flow rate relationships in the intact pulmonary vascular network of mice, we developed a protocol for measuring pulmonary vascular resistance and impedance in isolated, ventilated, and perfused mouse lungs. We used pulmonary emboli to validate the effect of vascular obstruction on resistanc...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-005-9050-z
更新日期：2006-04-01 00:00:00
abstract：:A mathematical model has been developed to investigate previously obtained experimental findings relating to the activation of factor X by surface-bound tissue factor--factor VIIa (TF:VIIa) in a tubular flow reactor. In those experiments, factor X was perfused through a microcapillary tube over a range of flow (shear)...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02660888
更新日期：1996-05-01 00:00:00
abstract：:In 12 dogs anesthetised with alpha-chloralose and urethane, and beta-adrenergic blocked with propranolol, the cervical vagi were stimulated for 60 seconds with supramaximal constant current pulses at frequencies between 2.0 and 10 Hz. The time course of the recovery of the cardiac period response, after cessation of v...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02584303
更新日期：1991-01-01 00:00:00
abstract：:The scarcity of data available on the best approach for pulmonary fetal valve replacement or implantation necessitate an investigation on whether practices using adult transcatheter valves could be translated to fetal applications. The objective of this study is to evaluate the hemodynamic characteristics and the turb...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02475-3
更新日期：2020-06-01 00:00:00
abstract：:An endovascular technique using a stent has been developed and successfully applied in the treatment of wide neck aneurysms. A stent can facilitate thrombosis in the aneurysm pouch while maintaining biocompatible passage of the parent artery. Insertion of the stent changes the flow characteristics inside the aneurysm ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1500406
更新日期：2002-07-01 00:00:00
abstract：:Development of increasingly complex integrated cellular systems will be a major challenge for the next decade and beyond, as we apply the knowledge gained from the sub-disciplines of regenerative medicine, synthetic biology, micro-fabrication and nanotechnology, systems biology, and developmental biology. In this pros...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-013-0902-7
更新日期：2014-02-01 00:00:00
abstract：:To better understand the relationship between kinetic processes of contraction and the dynamic features of an isometric twitch, studies were conducted using a mathematical model that included: (1) kinetics of cross bridge (XB) cycling; (2) kinetics of thin filament regulatory processes; (3) serial and feedback interac...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1366669
更新日期：2001-05-01 00:00:00
abstract：:Zebrafish is an emerging model system for cardiac conduction and regeneration. Zebrafish heart regenerates after 20% ventricular resection within 60 days. Whether cardiac conduction phenotype correlated with cardiomyocyte regeneration remained undefined. Longitudinal monitoring of the adult zebrafish heart (n = 12) wa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-9993-6
更新日期：2010-07-01 00:00:00
abstract：:Bending stiffness of passive-dynamic ankle-foot orthoses (PD-AFOs) is a functional characteristic thought to restore lost ankle function due to weakened plantar flexors. However, lower extremity impairment profiles of patients are seldom limited to plantar flexion weakness, and PD-AFO characteristics often influence g...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1067-8
更新日期：2015-02-01 00:00:00
abstract：:The effects of training on a person's ability to perceive, interpret and utilize information presented via the tactile sense were examined by using a dual-channel electrocutaneous tracking approach. The electrocutaneous code studied was linear pulse rate encoding of information. The stimulus had a pulse width of 200 m...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02367409
更新日期：1986-01-01 00:00:00
abstract：:Asymmetric 75% and 95% area reduction, transparent Sylgard stenotic models were operated under internal carotid artery (ICA) [Womersley parameter, alpha=5.36, Re(mean) =213 and 180, respectively, and Re(peak)=734 and 410, respectively] and left anterior descending coronary artery (LAD) flow wave forms (alpha=2.65, Re(...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.131
更新日期：1998-03-01 00:00:00
abstract：:The epidermal growth factor (EGF) is a growth factor with effects on many cell types and tissue. Morphometric and passive biomechanical properties were studied in isolated segments of the esophagus in 22 EGF-treated rats and 12 control rats. The rats were divided into groups with EGF treatment for 2, 4, 7, and 14 days...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1114/1.1575758
更新日期：2003-06-01 00:00:00
abstract：:Early intervention programs aim at improving cognitive and motor outcomes of preterm infants. Intensive custom-tailored training activities are usually accompanied by assessment procedures, which have shortcomings, such as subjectivity, complex setups, and need for structured environments. A novel sensorized system, c...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1669-4
更新日期：2016-12-01 00:00:00
abstract：:While considerable efforts have been expended to develop snore-driven markers for detecting obstructive sleep apnea (OSA), there is little emphasis on the relationship between the human upper airway (UA) dimensions and the attributes of snores. This paper aims to investigate the acoustical and perceptual impacts of ch...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-009-9745-7
更新日期：2009-09-01 00:00:00
abstract：:Arterial tissue is commonly assumed to be incompressible. While this assumption is convenient for both experimentalists and theorists, the compressibility of arterial tissue has not been rigorously investigated. In the current study we present an experimental-computational methodology to determine the compressibility ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-015-1417-1
更新日期：2016-04-01 00:00:00
abstract：:Much effort is expended in developing biomimetic scaffolds that provide the micro-architecture of native tissue with appropriate cellular niches. Such scaffolds are often seeded with progenitor cells to generate engineered replacements for diseased or damaged tissues. An alternative approach relies on biology, rather ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
doi：10.1007/s10439-014-1174-6
更新日期：2015-03-01 00:00:00
abstract：:We examine the low-frequency limit of hearing of the mammalian ear through the analytical modelling of the natural frequency of the tympanic membrane. The resulting equation of the natural frequency of the modelled tympanic membrane is numerically verified against previous theoretical studies, and is statistically val...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-019-02356-4
更新日期：2020-01-01 00:00:00
abstract：:Peripheral nerve injuries present challenges to regeneration. Currently, the gold standard for nerve repair is an autograft that results in another region of the body suffering nerve damage. Previously, bioactive borate glass (BBG) has been studied in clinical trials to treat patients with non-healing wounds, and we h...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1689-0
更新日期：2016-12-01 00:00:00
abstract：:Newborns with single ventricle physiology are usually palliated with a multi-staged procedure. When cardiovascular complications e.g., collateral vessel formation occur during the inter-stage periods, further treatments are required. An 8-month-old patient, who underwent second stage (i.e., bi-directional Glenn, BDG) ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-014-1113-6
更新日期：2015-06-01 00:00:00
abstract：:Measurement of the instantaneous pressure-volume relationship of the left ventricle is fundamental to the study of ventricular mechanics. In order to effectively investigate this relationship, it is necessary to vary and control the time course of ventricular volume change in a variety of prescribed manners. In the pa...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02367387
更新日期：1982-01-01 00:00:00
abstract：:The optimal means of electrically stimulating a skeletal muscle to contract around a fluid-filled pouch (i.e., a skeletal muscle ventricle [SMV]) has not been determined. A SMV was made from the latissimus dorsi muscle in five dogs and the rectus abdominis muscle in five dogs, and each SMV was electrically stimulated ...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02364611
更新日期：1990-01-01 00:00:00
