Effects of Chemically Doped Bioactive Borate Glass on Neuron Regrowth and Regeneration.

Abstract:

:Peripheral nerve injuries present challenges to regeneration. Currently, the gold standard for nerve repair is an autograft that results in another region of the body suffering nerve damage. Previously, bioactive borate glass (BBG) has been studied in clinical trials to treat patients with non-healing wounds, and we have reported that BBG is conducive for soft tissue repair. BBG provides structural support, degrades in a non-cytotoxic manner, and can be chemically doped. Here, we tested a wide range of chemical compounds that are reported to have neuroprotective characteristics to promote regeneration of peripheral neurons after traumatic injury. We hypothesized that chemical dopants added in trace amounts to BBG would improve neuronal survival and neurite outgrowth from dorsal root ganglion (DRG) explants. We measured neurite outgrowth from whole DRG explants, and survival rates of dissociated neurons and support cells that comprise the DRG. Results show that chemically doped BBGs have differentially variable effects on neuronal survival and outgrowth, with iron, gallium, and zinc improving outgrowth of neurons, and iodine causing the most detriment to neurons. Because chemically doped BBGs support increased nerve regrowth and survival, they show promise for use in peripheral nerve regeneration.

journal_name

Ann Biomed Eng

authors

Gupta B,Papke JB,Mohammadkhah A,Day DE,Harkins AB

doi

10.1007/s10439-016-1689-0

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

3468-3477

issue

12

eissn

0090-6964

issn

1573-9686

pii

10.1007/s10439-016-1689-0

journal_volume

44

pub_type

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