Abstract：
:We present a method of measuring force response of a single living cell, attached to a substrate, in situ, by a functionalized microelectromechanical systems sensor that applies local deformation on the cell. The sensor is a single crystal silicon microcantilever beam with prescribed shape and geometry, and is coated by a thin layer of fibronectin. It is brought in contact with a cell to form adhesion cites, and is then moved by a piezoactuator to deform the cell locally. The force is transmitted from the adhesion site(s) on the cantilever to the sites on the substrate through the cytoskeleton. The interaction force between the cell and the cantilever is measured from the deformation of the cantilever and its spring constant, which can be obtained by several independent means. The force and the cell deformation can be 10 s of nano-Newtons and micrometers, respectively. We demonstrate the method using two families of force sensors with spring constants of 18 and 0.4 nN/microm. Several cells, endothelial and fibroblast, are deformed by tens of micrometers until the adhesion sites failed. Their force-deformation response shows strong linearity. Several possible mechanisms are discussed to explain the linear response.
journal_name
Ann Biomed Engjournal_title
Annals of biomedical engineeringauthors
Saif MT,Sager CR,Coyer Sdoi
10.1114/1.1591189subject
Has Abstractpub_date
2003-09-01 00:00:00pages
950-61issue
8eissn
0090-6964issn
1573-9686journal_volume
31pub_type
杂志文章abstract：:Tendon stem cells (TSCs) may be used to effectively repair or regenerate injured tendons. However, the fates of TSCs once implanted in vivo remain unclear. This study was aimed to determine the feasibility of labeling TSCs with super-paramagnetic iron oxide (SPIO) nano-particles to track TSCs in vivo using MRI. Rabbit...
journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-013-0802-x
更新日期：2013-10-01 00:00:00
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02390377
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2004-04-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368022
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：1998-05-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-012-0722-1
更新日期：2013-04-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-016-1579-5
更新日期：2016-09-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 临床试验,杂志文章
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更新日期：2001-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-007-9350-6
更新日期：2007-10-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02368173
更新日期：1992-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-020-02522-z
更新日期：2020-07-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-018-02137-5
更新日期：2019-01-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0231-z
更新日期：2011-03-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章,评审
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journal_title：Annals of biomedical engineering
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2009-05-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/s10439-010-0050-2
更新日期：2010-09-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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更新日期：2007-08-01 00:00:00
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
doi：10.1007/BF02364168
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journal_title：Annals of biomedical engineering
pub_type： 杂志文章
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journal_title：Annals of biomedical engineering
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更新日期：2003-12-01 00:00:00
