Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.

Abstract:

:In order to explore the effects of hydrogen sulfide (H2S) on myocardial fibrosis in diabetic rats and its underlying mechanisms, intraperitoneal injections of streptozotocin were used to establish the diabetes models and sodium hydrosulfide (NaHS) was used as an exogenous donor of H2S. Eight weeks later, Van Gieson staining was used to observe pathological changes, and basic hydrolysis methods were adopted to measure hydroxyproline content, while Western Blot was used to determine the expression of MMP2, MMP7, MMP11, MMP13, MMP16, TIMP1 and TGFβ1.The results showed that significant myocardial fibrosis, decreased TIMP1 and MMP2 expression and increased MMP7, MMP11, MMP13, MMP16 expressions occurred in diabetic group, but all these changes were significantly reversed in diabetic rats after NaHS treatment. This suggests that H2S could attenuate cardiac fibrosis induced by diabetes and its mechanisms may be related to its modulation of MMPs/TIMPs expression and regulation of TGFβ1.

journal_name

Biomed Mater Eng

authors

Xiao T,Zeng O,Luo J,Wu Z,Li F,Yang J

doi

10.3233/BME-151508

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

S2033-9

eissn

0959-2989

issn

1878-3619

pii

BME1508

journal_volume

26 Suppl 1

pub_type

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