Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells.

Abstract:

:Mesenchymal stem cells (MSCs) are a common tool in regenerative medicine. The nanoscale extracellular vesicles (nEVs) secreted by these cells were recently brought up to light thanks to their therapeutic potential. In this study, we assessed the in vitro behaviour of human umbilical vein endothelial cells (HUVECs) exposed to nEVs derived from human umbilical cord mesenchymal stem cells (hUC-MSCs). Nanoscale extracellular vesicles were isolated and characterized by NanoSight® and flow cytometry. HUVECs were stimulated with various concentrations of nEVs. To assess nEV interactions with HUVECs, confocal microscopy and angiogenesis assay were performed. The use of nEVs derived from hUC-MSCs was able to produce positive outcomes on HUVECs by acting on their angiogenic potential.

journal_name

Biomed Mater Eng

authors

Dostert G,Willemin AS,Jouan-Hureaux V,Louis H,Hupont S,Gillet P,Menu P,Decot V,Moby V,Velot É

doi

10.3233/BME-171626

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

S75-S79

issue

s1

eissn

0959-2989

issn

1878-3619

pii

BME1626

journal_volume

28

pub_type

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