Abstract：
BACKGROUND:Great potential of cellular therapies has generated extensive research in the field of cells harvesting and culturing. Transplantation of cell cultures has been used in a variety of therapeutic programs but in many cases it appeared that biomaterial scaffold or sheath would enhance cells regenerative potential. OBJECTIVE:Hydrogels composed of different proportions sodium and calcium alginates, were undertaken to evaluate their influence on mesenchymal stem cells and olfactory bulb-derived glial cells cultures. Additionally, these biomaterials were also enriched with fibrin protein. METHODS:The structure of materials was visualized by means of scanning electron microscopy. After seeding with cells - hydrogels were observed with inverted and fluorescence microscope. Cell's morphology, behavior and phenotype were analyzed in investigated materials by means of light, fluorescence and scanning electron microscopes. Also, viability assay was performed with Alamar Blue cytotoxic test. RESULTS:Our observations showed that basic alginate hydrogels had significant influence on both cell types. Materials maintained cells alive, which is desired attribute, however none of them kept cells in normal, flat form. Alginates with significant calcium component kept cells alive for longer period of culture. CONCLUSIONS:Addition of fibrin protein resulted in material's biocompatibility properties improvement, by creation of adhesion surface, which helps cells to keep proper morphology and behavior. Our findings suggest that addition of fibrin protein to alginate hydrogels improves them as cell carriers for regenerative medicine applications.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Marycz K,Szarek D,Grzesiak J,Wrzeszcz Kdoi
10.3233/BME-140966subject
Has Abstractpub_date
2014-01-01 00:00:00pages
1625-37issue
3eissn
0959-2989issn
1878-3619pii
V03L535424308484journal_volume
24pub_type
杂志文章abstract：:Hydroxyapatite is widely utilized as a component in dental hygiene agents. The ability to adsorb and remove dental plaque adhering to tooth surfaces is recognized as an important property of hydroxyapatite particles. In this study, the adsorptive ability of hydroxyapatite was evaluated based on its adsorptive amount a...
journal_title：Bio-medical materials and engineering
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更新日期：2014-01-01 00:00:00
abstract：:The selection of best material pair in the hip prosthetics design for improved performance and life relies on the estimation of hip joint contact stresses and contact pressure distribution during various dynamic loading cycles: Climbing Upstairs, Climbing downstairs and Knee bending. The maximum Von Mises stress, cont...
journal_title：Bio-medical materials and engineering
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doi：10.3233/BME-181019
更新日期：2018-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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doi：10.3233/BME-130811
更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141133
更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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更新日期：2006-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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pub_type： 临床试验,杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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journal_title：Bio-medical materials and engineering
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更新日期：2006-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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doi：
更新日期：1997-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141163
更新日期：2014-01-01 00:00:00
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更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141083
更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
pub_type： 杂志文章
doi：10.3233/BME-141252
更新日期：2015-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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doi：10.3233/BME-2012-0687
更新日期：2012-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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doi：
更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Bio-medical materials and engineering
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journal_title：Bio-medical materials and engineering
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更新日期：2000-01-01 00:00:00
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