A simple two dimensional culture method to study the hypertrophic differentiation of rat articular chondrocytes.

Abstract:

OBJECTIVES:Chondrocytes hypertrophy is a physiological process observed in endochondral ossification during development until adolescence in human. It can also be observed during pathophysiological conditions such as osteoarthritis. Hypertrophic chondrocytes synthesise collagen X and express matrix metalloproteinase 13 and alkaline phosphatase. The cellular models available to study this process are either not convenient, they might lead to a rapid dedifferentiation of chondrocytes, or they are far from the physiological conditions. The objective of this study was to design an user-friendly 2D-primary cell culture of young articular chondrocytes of rat able to follow the terminal differentiation process. EXPERIMENTAL DESIGN:After confluence, chondrocytes were cultured according to 4 differentiation protocols. Protocol 1 contained DMEM/F12 supplemented with 10% foetal bovine serum (FBS) and 2 μg/ml insulin. Protocol 2 contained alpha-MEM supplemented with 5% FBS and 2 μg/ml insulin. Protocol 3 contained 2% FBS and 2 μg/ml insulin. Protocol 4 contained DMEM/F12 supplemented with 2% FBS in absence or in presence of 2 μg/ml insulin and 37.5 μg/ml ascorbate. The cell morphology was observed by phase-contrast microscopy and the expression of markers specific of mature and hypertrophic chondrocytes were assessed by RT-qPCR. RESULTS:The effect of a decrease in nutrient quality of the culture medium after confluence was tested using protocols 1, 2 and 3. Protocol 1 did not allow the maintenance of chondrocyte phenotype more than one week, because cells became fibroblastic. A decrease in Sox9 mRNA expression, in collagen II/collagen I and in aggrecan/versican mRNA ratios was also found with protocol 1. Protocol 3 was the best when compared with protocols 1 and 2. It allowed chondrocytes to adopt a hypertrophic morphology. Cells also expressed the collagen X specific hypertrophic marker, and presented an increase in collagen II/I and aggrecan/versican ratios after 15 days of culture post-confluence. The effect of the insulin/ascorbate supplementation was studied using protocol 4. The insulin/ascorbate supplementation allowed an earlier chondrocytes conversion to terminal differentiation with a prolonged effect till 3 weeks post-confluence, compared to control without insulin/ascorbate. Finally, the profile of chondrocyte differentiation was checked during 5 successive sub-cultures. Only the first passage could be used to study hypertrophy. CONCLUSION:A convenient protocol to study chondrocyte hypertrophy is proposed. Protocol 4 offers the possibility to study this differentiation phenotype which is crucial for the development of articular diseases such as osteoarthritis. Our model could also be used in tissue engineering for cartilage repair strategies in which hypertrophic differentiation of chondrocyte should be avoided.

journal_name

Biomed Mater Eng

authors

Filip A,Bianchi A,Mainard D,Lacolley P,Magdalou J,Mercier N

doi

10.3233/BME-141252

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

87-102

issue

1 Suppl

eissn

0959-2989

issn

1878-3619

pii

C1175442X7W5H727

journal_volume

25

pub_type

杂志文章
  • Experimal study of young male drivers' responses to vehicle collision using EMG of lower extremity.

    abstract::A driver's response to a front-coming vehicle collision consists of braking reaction time and braking behavior. The purpose was to investigate drivers' responses at different speeds, relative distances, and particularly the behavior on the accelerator at the collision moment. Twelve young men participated in driving s...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151347

    authors: Gao Z,Li C,Hu H,Zhao H,Chen C,Yu H

    更新日期:2015-01-01 00:00:00

  • Optically-guided scalpel with light-scattering module for carpal tunnel surgical procedure via minimally invasive surgery.

    abstract::A novel technique and product for carpal tunnel microscopic surgical procedures using a modified medical device containing a head as a surgical scalpel under light guidance were designed and studied. The novel design of the medical device was developed and applied in a new carpal tunnel microscopic surgical procedure ...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151303

    authors: Chiang MC,Huang CC

    更新日期:2015-01-01 00:00:00

  • Renal epithelia in long term gradient culture for biomaterial testing and tissue engineering.

    abstract::In the organism epithelia perform perfect barrier functions. Strong rheological and mechanical influences constitute the normal environment of this tissue throughout life. Most epithelia are exposed to different fluids at the luminal and basal sides. To obtain realistic information about tissue development in modern b...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Minuth WW,Schumacher K,Strehl R

    更新日期:2005-01-01 00:00:00

  • Improved bond performance of a dental adhesive system using nano-technology.

    abstract::Since adhesive technology was introduced into dental field, metal-based restoration has been gradually replaced by metal-free restoration. Using the adhesive technology, minimum invasive technique has been possible in daily clinical practice as well as esthetic tooth-colored restorations have become very popular all o...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章,评审

    doi:10.3233/BME-2009-0587

    authors: Nagano F,Selimovic D,Noda M,Ikeda T,Tanaka T,Miyamoto Y,Koshiro K,Sano H

    更新日期:2009-01-01 00:00:00

  • Preparation of size-controlled silver nanoparticles and chitosan-based composites and their anti-microbial activities.

    abstract::We previously reported a simple method for the preparation of size-controlled spherical silver nanoparticles (Ag NPs) generated by autoclaving a mixture of silver-containing glass powder and glucose. The particle size is regulated by the glucose concentration, with concentrations of 0.25, 1.0 and 4.0 wt% glucose provi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130772

    authors: Nguyen VQ,Ishihara M,Mori Y,Nakamura S,Kishimoto S,Fujita M,Hattori H,Kanatani Y,Ono T,Miyahira Y,Matsui T

    更新日期:2013-01-01 00:00:00

  • Ultrathin polymer monolayers for promotion of cell growth on bioprosthetic materials -- evolution of a new concept to improve long term performance of biologic heart vales.

    abstract::Reoperation of aldehyde tanned bioprotheses due to calcific degeneration remains their major drawback. Based on experiments studying mechanisms and factors that influence the time phase, extent and progression of calcification and evaluating the efficiency of anticalcification treatments and the effects of surface see...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Dahm M,Rühe J,Berchthold B,Prüfer D,Prucker O,Chang BJ,Wallrath A,Oelert H

    更新日期:2004-01-01 00:00:00

  • Accelerating sino-atrium computer simulations with graphic processing units.

    abstract::Sino-atrial node cells (SANCs) play a significant role in rhythmic firing. To investigate their role in arrhythmia and interactions with the atrium, computer simulations based on cellular dynamic mathematical models are generally used. However, the large-scale computation usually makes research difficult, given the li...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151365

    authors: Zhang H,Xiao Z,Lin SF

    更新日期:2015-01-01 00:00:00

  • Extracellular matrices as advanced scaffolds for vascular tissue engineering.

    abstract::An alternative non-vascular extracellular material was obtained by decellularisation of porcine urinary bladder and examined for its potential as scaffold for vascular tissue engineering. Analysis using Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Laser Scanning Microscopy (LSCM) showed a poro...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-2009-0598

    authors: Piterina AV,Callanan A,Davis L,Meaney C,Walsh M,McGloughlin TM

    更新日期:2009-01-01 00:00:00

  • Titanium-porcelain system. Part II: Bond strength of fired porcelain on nitrided pure titanium.

    abstract::Commercially pure titanium (CPT) substrate was subjected to porcelain firing and bond strengths under three-point bending mode (span length: 15 mm; crosshead speed: 0.5 mm/min) were evaluated. Experimental variables included surface treatments of CPT and porcelain firing schedules. Variables for the surface treatments...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Oshida Y,Fung LW,Isikbay SC

    更新日期:1997-01-01 00:00:00

  • The dynein-triggered ciliary motion in embryonic nodes: an exploratory study based on computational models.

    abstract::The cilia, presenting a rotational movement in the embryonic nodes, play a crucial role in the left-right specification during embryogenesis. The characteristic architecture of these cilia is based on a cylindrical arrangement of 9 doublet microtubules and the motion of the cilia is triggered by the dynein motors loca...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141063

    authors: Chen D,Zhong Y,Shinohara K,Nishida T,Hasegawa T,Hamada H

    更新日期:2014-01-01 00:00:00

  • Patient specific respiratory motion modeling using a limited number of 3D lung CT images.

    abstract::To build a patient specific respiratory motion model with a low dose, a novel method was proposed that uses a limited number of 3D lung CT volumes with an external respiratory signal. 4D lung CT volumes were acquired for patients with in vitro labeling on the upper abdominal surface. Meanwhile, 3D coordinates of in vi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141133

    authors: Cui X,Gao X,Xia W,Liu Y,Liang Z

    更新日期:2014-01-01 00:00:00

  • Ultrasonic study of processed milk cream and vegetable oils.

    abstract::Food systems are complex and inhomogeneous in nature. Ultrasonic propagation velocity in the food systems, in this case processed milk cream and vegetable oils, is studied. Ultrasonic properties of the food systems are similar to those of complex biological tissues. The presence of vegetable oil as an adulterant in th...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Singh VR,Kumar A

    更新日期:1992-04-01 00:00:00

  • Locking strength of Morse tapers used for modular segmental bone defect replacement prostheses.

    abstract::Mechanical testing has been performed to characterize the locking strength of Morse taper locks used for reconstruction of large bone defects. Taper joint pairs were locked with a series of compressive loads increasing from 500 to 3500 N. Following each load application the taper locks were distracted with either an a...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Duda GN,Elias JJ,Valdevit A,Chao EY

    更新日期:1997-01-01 00:00:00

  • Computer-aided detection of lung nodules using outer surface features.

    abstract::In this study, a computer-aided detection (CAD) system was developed for the detection of lung nodules in computed tomography images. The CAD system consists of four phases, including two-dimensional and three-dimensional preprocessing phases. In the feature extraction phase, four different groups of features are extr...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151418

    authors: Demir Ö,Yılmaz Çamurcu A

    更新日期:2015-01-01 00:00:00

  • Optimal subband Kalman filter for normal and oesophageal speech enhancement.

    abstract::This paper presents the single channel speech enhancement system using subband Kalman filtering by estimating optimal Autoregressive (AR) coefficients and variance for speech and noise, using Weighted Linear Prediction (WLP) and Noise Weighting Function (NWF). The system is applied for normal and Oesophageal speech si...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141183

    authors: Ishaq R,García Zapirain B

    更新日期:2014-01-01 00:00:00

  • Effect of platelet-rich plasma on bone engineering with an alloplastic substitute containing BMP2.

    abstract::A robust method for inducing bone-formation without an autograft has not been established. Currently, both platelet-rich plasma (PRP) and bone morphogenetic protein (BMP) have been widely investigated for their clinical use in such cases. However, their synergistic effect is still controversial and previously shown di...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130741

    authors: Yoshida K,Sumita Y,Marukawa E,Harashima M,Asahina I

    更新日期:2013-01-01 00:00:00

  • Thyroid hormone disorders and sepsis.

    abstract::Sepsis is a systemic inflammatory response syndrome with high mortality, which results from severe infection and can lead to secondary organ dysfunction. It is one of the most common cause of death in intensive care unit. Clinical reports have shown that sepsis was often accompanied by thyroid dysfunction, which is ca...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章,评审

    doi:10.3233/BME-171646

    authors: Luo B,Yu Z,Li Y

    更新日期:2017-01-01 00:00:00

  • In vivo study of osteogenerating properties of calcium-phosphate coating on titanium alloy Ti-6Al-4V.

    abstract::The method of formation of bioactive calcium-phosphate coating on medical titanium alloy Ti-6Al-4V (3.5-5.3% V; 5.3-6.8% Al; balance -Ti) by plasma electrolytic oxidation (PEO) has been developed. Evaluation of osteogenerating properties of the coating at fractures of the shaft of the femur on Wistar line laboratory r...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-161608

    authors: Gnedenkov SV,Sinebryukhov SL,Puz AV,Egorkin VS,Kostiv RE

    更新日期:2016-01-01 00:00:00

  • The development of a physiological hip prosthesis: evaluation of the strains after implantation of a prototype of hip implant: experiment in a dry femur.

    abstract::Based upon previous research on the relation between hip prosthesis designs and strain distributions in a proximal femur model, a prototype of a "physiological" hip prosthesis was designed and manufactured. Strain gauge measurements on a dry femur before and after implantation of this prosthesis were made in different...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Vander Sloten J,Labey L,Van Audekercke R,Van der Perre G

    更新日期:1993-04-01 00:00:00

  • A study of load cycling in a NiTi shape memory alloy with pseudoelastic behaviour used in dental prosthetic fixators.

    abstract::NiTi alloy used in dental prosthetic fixators shows pseudoelastic behaviour and exhibits a great potential in dental and orthopaedic applications where constant correcting loads are required. In order to use such materials in dental prosthetic fixators, where the device is cyclically deformed, it is necessary to inves...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Sabrià J,Cortada M,Giner L,Gil FJ,Fernández E,Manero JM,Planell JA

    更新日期:1996-01-01 00:00:00

  • An experimental study on the biomechanical properties of the cancellous bones of distal femur.

    abstract:OBJECTIVE:To study the comprehensive biomechanical properties of the cancellous bone of distal femur through a series of mechanical tests, and provide relevant subjects with the basic technical data. BACKGROUND:The study on bone mechanics is a commonly used approach to evaluate the biomechanical competency of bone. Th...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Du C,Ma H,Ruo M,Zhang Z,Yu X,Zeng Y

    更新日期:2006-01-01 00:00:00

  • Theoretical foundation on a noninvasive estimation for viscoelastic mechanical property of blood vessels by ultrasonic Doppler effect.

    abstract::The method used in this paper is developed to estimate the degree of viscoelasticity of the blood vessel wall in a noninvasive mechanical method. In this proposed method, we can obtain an output waveform connected with mechanical behavior of blood vessels by using Ultrasonic Doppler Effect Sensory (UDES). Characterizi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Yoshinari H,Yokobori AT Jr,Ohkuma T

    更新日期:1994-01-01 00:00:00

  • Experimental evaluation and theoretical modeling of oxygen transfer rate for the newly developed hollow fiber bioreactor with three compartments.

    abstract::Bioartificial liver support provides a bridge to transplantation which is at present the only proven specific treatment for acute liver failure. In this paper, a novel multi-coaxial hollow fiber bioreactor so-called "Fibre-in-Fibre FIF Bioartificial liver device" with three compartments is experimentally and mathemati...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130762

    authors: Hilal-Alnaqbi A,Mourad AH,Yousef BF,Gaylor JD

    更新日期:2013-01-01 00:00:00

  • In vivo rat knee cartilage volume measurement using quantitative high resolution MRI (7 T): feasibility and reproducibility.

    abstract:OBJECTIVES:to assess reliability and reproducibility of quantitative MRI (7 T) in assessing rat femoro-tibial cartilage volume. METHODS:5 healthy rat knees were scanned in vivo using a 7 T experimental imager. Sagittal high resolution 3D Gradient Echo with fat suppression sequences were performed with a dedicated home...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Goebel JC,Bolbos R,Pinzano A,Schaeffer M,Rengle A,Galois L,Etienne S,Netter P,Loeuille D,Beuf O,Gillet P

    更新日期:2008-01-01 00:00:00

  • Stability of gabexate mesilate products: Influence of the addition of mannitol.

    abstract::Gabexate mesilate is a non-peptide protease inhibitor, developed in Japan, which is used in the treatment of acute pancreatitis and disseminated intravascular coagulation. This compound is readily hydrolyzed as it has ester bonds in its structure. It is now out of patent in Japan and there are many generic versions on...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-2010-0611

    authors: Sakurai M,Abe H,Okamura N,Inoue Y,Akiyoshi T,Matsuyama K,Uchida T,Otsuka M

    更新日期:2010-01-01 00:00:00

  • Improved walking ability with wearable robot-assisted training in patients suffering chronic stroke.

    abstract::Wearable robotic devices provide safe and intensive rehabilitation, enabling repeated motions for motor function recovery in stroke patients. The aim of this small case series was to demonstrate the training effects of a three-week robotic leg orthosis, and to investigate possible mechanisms of the sensory-motor alter...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151320

    authors: Li L,Ding L,Chen N,Mao Y,Huang D,Li L

    更新日期:2015-01-01 00:00:00

  • Surface degradation study of magnesium tested in simulated body fluid.

    abstract::Magnesium has attracted a lot of attention as a new class of biodegradable material. The surface properties of magnesium in simulated body fluid (SBF) were investigated in the current research work. Cast magnesium samples with different surface characteristics were prepared which were then placed in SBF for 2, 4 and 8...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-191057

    authors: Sana A,Malik I,Mujahid M,Akram MA,Adeel Umer M

    更新日期:2019-01-01 00:00:00

  • Failures of yttria-stabilised tetragonal zirconia: 52 retrieved ceramic femoral heads of total hip prostheses.

    abstract::Fifty two ceramic femoral heads have been investigated after implantation. All heads had articulated against ultra high molecular weight polyethylene (UHMWPE) cups. Examination of the heads shows changes in sphericity and surface roughness due to the wear. The behaviour of these retrieved heads after clinical use conf...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Fernández-Fairén M,Sala P,Gil FJ

    更新日期:2006-01-01 00:00:00

  • Effect of contacts configuration and location on selective stimulation of cuff electrode.

    abstract:BACKGROUND:Cuff electrodes have been widely used chronically in different clinical applications. Advancements have been made in selective stimulation by using multi-contact cuff electrodes. Steering anodic current is a strategy to increase selectivity by reshaping and localizing electric fields. There are two configura...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151281

    authors: Taghipour-Farshi H,Frounchi J,Ahmadiasl N,Shahabi P,Salekzamani Y

    更新日期:2015-01-01 00:00:00

  • Abrasive wear of ceramic, metal, and UHMWPE bearing surfaces from third-body bone, PMMA bone cement, and titanium debris.

    abstract::The debris generated by the progressive wear of total joint replacement (TJR) devices is considered a primary cause of osteolysis, bone resorption, and premature failure of artificial hips and knees. The vast majority of this debris originates from the UHMWPE articulating surfaces caused by tribological interaction wi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Davidson JA,Poggie RA,Mishra AK

    更新日期:1994-01-01 00:00:00