Preparation of size-controlled silver nanoparticles and chitosan-based composites and their anti-microbial activities.

Abstract:

:We previously reported a simple method for the preparation of size-controlled spherical silver nanoparticles (Ag NPs) generated by autoclaving a mixture of silver-containing glass powder and glucose. The particle size is regulated by the glucose concentration, with concentrations of 0.25, 1.0 and 4.0 wt% glucose providing small (3.48 ± 1.83 nm in diameter), medium (6.53 ± 1.78 nm) and large (12.9 ± 2.5 nm) particles, respectively. In this study, Ag NP/chitosan composites were synthesized by mixing each of these three Ag NP suspensions with a 75% deacetylated (DAc) chitosan suspension (pH 5.0) at room temperature. The Ag NPs were homogeneously dispersed and stably embedded in the chitosan matrices. The Ag NP/chitosan composites were obtained as yellow or brown flocs. It was estimated that approximately 60, 120 and 360 μg of the small, medium and large Ag NPs, respectively, were maximally embedded in 1 mg of chitosan. The bactericidal and anti-fungal activities of the Ag NP/chitosan composites increased as the amount of Ag NPs in the chitosan matrix increased. Furthermore, smaller Ag NPs (per weight) in the chitosan composites provided higher bactericidal and anti-fungal activities.

journal_name

Biomed Mater Eng

authors

Nguyen VQ,Ishihara M,Mori Y,Nakamura S,Kishimoto S,Fujita M,Hattori H,Kanatani Y,Ono T,Miyahira Y,Matsui T

doi

10.3233/BME-130772

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

473-83

issue

6

eissn

0959-2989

issn

1878-3619

pii

980N05U0XNH27155

journal_volume

23

pub_type

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