Hollow calcium phosphate microcarriers for bone regeneration: in vitro osteoproduction and ex vivo mechanical assessment.

Abstract:

:Synthetic grafting materials, such as calcium phosphates (hydroxyapatite, HA; tricalcium phosphate, TCP), polymers, or composites thereof, can be used as osteoconductive scaffolds and delivery vehicles for osteoinductive growth factors. Carrier materials must be engineered to deliver these factors in a controlled fashion at a rate and dose consistent with the biological need and responsiveness of the system to optimize bone formation and ingrowth. They should also simultaneously provide mechanical support and slowly resorb as new bone is formed. This investigation assessed the elution characteristics of BMP-7 (OP-1) from hollow calcium phosphate spheres of varying chemical composition (HA/beta-TCP) and porosity (dense/porous). The pharmacokinetics indicated a bimodal trend of protein release with protein elution peaking between fifteen and thirty minutes in solution (bolus release) and continuing through the eight-week time point (sustained release). Eluted OP-1 bioactivity was characterized over a three-week period using mesenchymal stem cell (MSC) cultures and included assessment of the protein's differential, proliferative, and calcified nodule forming abilities. Alkaline phosphatase enzyme (ALP) activity in MSCs peaked between 12 and 16 days post-OP-1 exposure. Elutant from the HA dense treatment group induced the highest degree of ALP expression while elutant from the beta-TCP treatment groups induced the formation of significantly higher numbers of calcified nodules in culture. The aggregate modulus of a clinically relevant 2 cc dose of carriers was quantified using custom designed testing fixtures to investigate the effects of carrier size, porosity, chemical composition, and the presence of a central hole on mechanical integrity. Significant increases in moduli were noted for carrier size and chemical composition (HA>beta-TCP). These preliminary in vitro and ex vivo results indicate the clinical potential of the hollow calcium phosphate carriers as successful load-bearing delivery vehicles for OP-1.

journal_name

Biomed Mater Eng

authors

Santoni BG,Pluhar GE,Motta T,Wheeler DL

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

277-89

issue

5

eissn

0959-2989

issn

1878-3619

journal_volume

17

pub_type

杂志文章
  • Individual tooth region segmentation using modified watershed algorithm with morphological characteristic.

    abstract::In this paper, a new method for individual tooth segmentation was proposed. The proposed method is composed of enhancement and extraction of boundary and seed of watershed algorithm using trisection areas by morphological characteristic of teeth. The watershed algorithm is one of the conventional methods for tooth seg...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141153

    authors: Na SD,Lee G,Lee JH,Kim MN

    更新日期:2014-01-01 00:00:00

  • Experimental evaluation and theoretical modeling of oxygen transfer rate for the newly developed hollow fiber bioreactor with three compartments.

    abstract::Bioartificial liver support provides a bridge to transplantation which is at present the only proven specific treatment for acute liver failure. In this paper, a novel multi-coaxial hollow fiber bioreactor so-called "Fibre-in-Fibre FIF Bioartificial liver device" with three compartments is experimentally and mathemati...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130762

    authors: Hilal-Alnaqbi A,Mourad AH,Yousef BF,Gaylor JD

    更新日期:2013-01-01 00:00:00

  • Cultured blood versus donated blood: long-run perspectives of the economy of blood.

    abstract::Recent advances of fundamental research on the in vitro generation of red blood cells (RBCs) from hematopoietic stem cells in the laboratory open new possibilities of the utilization of cultured RBCs in transfusion medicine. We study the economic challenge of the setup and development of the mass industrial production...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141251

    authors: Mercier Ythier J

    更新日期:2015-01-01 00:00:00

  • Computer-aided detection of lung nodules using outer surface features.

    abstract::In this study, a computer-aided detection (CAD) system was developed for the detection of lung nodules in computed tomography images. The CAD system consists of four phases, including two-dimensional and three-dimensional preprocessing phases. In the feature extraction phase, four different groups of features are extr...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151418

    authors: Demir Ö,Yılmaz Çamurcu A

    更新日期:2015-01-01 00:00:00

  • Preparation of self-assembled nanoparticles of chitosan oligosaccharide-graft-polycaprolactone as a carrier of bovine serum albumin drug.

    abstract::Chitosan oligosaccharides graft polycaprolactone copolymer (PHCSO-g-PCL) has been synthesized via initiating the polymerization of e-caprolactone (CL) monomer through an amino group protection route using phthaloyl chitosan oligosaccharide (PHCSO) as intermediate. The grafting reaction was carried out in Pyridine at 1...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141013

    authors: Li F,Zhang X,Li H,Xiang L,Chen Y

    更新日期:2014-01-01 00:00:00

  • A novel registration method for image-guided neurosurgery system based on stereo vision.

    abstract::This study presents a novel spatial registration method of Image-guided neurosurgery system (IGNS) based on stereo-vision. Images of the patient's head are captured by a video camera, which is calibrated and tracked by an optical tracking system. Then, a set of sparse facial data points are reconstructed from them by ...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151391

    authors: An Y,Wang M,Song Z

    更新日期:2015-01-01 00:00:00

  • An experimental study on the biomechanical properties of the cancellous bones of distal femur.

    abstract:OBJECTIVE:To study the comprehensive biomechanical properties of the cancellous bone of distal femur through a series of mechanical tests, and provide relevant subjects with the basic technical data. BACKGROUND:The study on bone mechanics is a commonly used approach to evaluate the biomechanical competency of bone. Th...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Du C,Ma H,Ruo M,Zhang Z,Yu X,Zeng Y

    更新日期:2006-01-01 00:00:00

  • Predicting the minimal inhibitory concentration of fluoroquinolones for Escherichia coli using an accumulation model.

    abstract::The objective of this study is to evaluate whether the accumulation model developed by Zarfl et al. (2008) could be used to predict the minimal inhibitory concentration (MIC) of a group of antibacterial fluoroquinolones (FQs) for Escherichia coli (E. coli). Our model, which is based on the "Fick-Nernst-Planck" equatio...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141203

    authors: Zhang W,Wang C,Tam KY

    更新日期:2014-01-01 00:00:00

  • A novel model for biomechanical behavior of human brain in epidural hematoma injuries.

    abstract::To obtain an appropriate model for the simulation of the biomechanical behavior of brain tissue and the deformation of ventricles, in particular, we have developed a novel computerized plain strain finite element model. For optimum results, a multiple loading solutions approach using various tissue parameters for the ...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Farmanzad F,Najarian S,Eslamee MR,Seddighi AS

    更新日期:2007-01-01 00:00:00

  • A study of quality control method for IMRT planning based on prior knowledge and novel measures derived from both OVHs and DVHs.

    abstract::Intensity-Modulated Radiation Therapy (IMRT) mathematically forms a large-scale optimization problem. The development of an IMRT plan is computationally expensive resulting in time-consuming, inefficient, and difficult to develop high-quality IMRT plans. By combining prior knowledge with proposed novel measures derive...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141173

    authors: Zhou Z,Chen Y,Yu Z,Wang D,Zhao C,Xu J,Song W,Li B,Shen J,Zhu X

    更新日期:2014-01-01 00:00:00

  • Controlled release bevacizumab in thermoresponsive hydrogel found to inhibit angiogenesis.

    abstract::Age-related macular degeneration (ARMD) and intraocular neovascular diseases have been treated clinically by anti-VEGF antibody drug bevacizumab. However, the use of bevacizumab in the treatment of retinal neovascular diseases has been limited due to the short half-life and frequent injections. In this research, novel...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141003

    authors: Hu CC,Chaw JR,Chen CF,Liu HW

    更新日期:2014-01-01 00:00:00

  • The influence of Zn on the deposition of HA on sol-gel derived bioactive glass.

    abstract::To evaluate the influence of Zn on the formation of hydroxyapatite (HA), zinc-containing glass 58S4Z was prepared by substitution of CaO with 4 wt% ZnO in 58S bioactive glass, and Zn-containing simulated body fluid (ZnSBF) was prepared as soaking fluid. During the soaking, all samples could induce the deposition of HA...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Du RL,Chang J

    更新日期:2006-01-01 00:00:00

  • A comparison study on the flexural strength and compressive strength of four resin-modified luting glass ionomer cements.

    abstract::The purpose of this study is to compare the differences in flexural strength and compressive strength between four resin-modified luting glass ionomer cements that are commonly used in clinics. Furthermore, this study investigates the influence of curing mode on the flexural strength and compressive strength of dual-c...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151284

    authors: Li Y,Lin H,Zheng G,Zhang X,Xu Y

    更新日期:2015-01-01 00:00:00

  • Degradability in vitro of polyurethanes based on synthetic atactic poly[(R,S)-3-hydroxybutyrate].

    abstract::The aim of the present study was to determine the degradability of aliphatic polyurethanes, based on a different amount of synthetic, atactic poly[(R,S)-3-hydroxybutyrate] (a-PHB), in hydrolytic (phosphate buffer) and oxidative (H2O2/CoCl2) solutions. The soft segments were built with atactic poly[(R,S)-3-hydroxybutyr...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151262

    authors: Brzeska J,Janeczek H,Janik H,Kowalczuk M,Rutkowska M

    更新日期:2015-01-01 00:00:00

  • A study of load cycling in a NiTi shape memory alloy with pseudoelastic behaviour used in dental prosthetic fixators.

    abstract::NiTi alloy used in dental prosthetic fixators shows pseudoelastic behaviour and exhibits a great potential in dental and orthopaedic applications where constant correcting loads are required. In order to use such materials in dental prosthetic fixators, where the device is cyclically deformed, it is necessary to inves...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Sabrià J,Cortada M,Giner L,Gil FJ,Fernández E,Manero JM,Planell JA

    更新日期:1996-01-01 00:00:00

  • Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.

    abstract::In order to explore the effects of hydrogen sulfide (H2S) on myocardial fibrosis in diabetic rats and its underlying mechanisms, intraperitoneal injections of streptozotocin were used to establish the diabetes models and sodium hydrosulfide (NaHS) was used as an exogenous donor of H2S. Eight weeks later, Van Gieson st...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151508

    authors: Xiao T,Zeng O,Luo J,Wu Z,Li F,Yang J

    更新日期:2015-01-01 00:00:00

  • A bioelectrical impedance phase angle measuring system for assessment of nutritional status.

    abstract::Bioelectrical impedance phase angle has been recommended as a tool to assess nutrition state, but there are no measuring devices have been specially designed for hospital residents. In this study, a system was established for the measurement of bioelectrical impedance phase angle. The electrical composition, calculati...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141193

    authors: Zhang G,Huo X,Wu C,Zhang C,Duan Z

    更新日期:2014-01-01 00:00:00

  • Does adipose tissue cultured with collagen matrix and preadipocytes give comparable results to the standard technique in plastic surgery?

    abstract:INTRODUCTION:Repairing contour defects is a challenge in plastic surgery. Different filling materials have been used with inadequate results and complications. The autologous fat transfer is the standard technique at the moment, but adipose tissue reserves are limited. The aim of our study was to compare in vivo on an ...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Mojallal A,Lequeux C,Auxenfans C,Braye F,Damour O

    更新日期:2008-01-01 00:00:00

  • 4D model of hemodynamics in the abdominal aorta.

    abstract::The paper presents an application of the FSI technique to determine hemodynamics in the abdominal aorta (AA). To establish boundary conditions for the FSI study, MR anatomical data and 4D MRI velocity-mapping data (in three blood flow velocity directions and time) were collected to acquire realistic geometry of the AA...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151312

    authors: Zbicinski I,Veshkina N,Stefańczyk L

    更新日期:2015-01-01 00:00:00

  • A simple two dimensional culture method to study the hypertrophic differentiation of rat articular chondrocytes.

    abstract:OBJECTIVES:Chondrocytes hypertrophy is a physiological process observed in endochondral ossification during development until adolescence in human. It can also be observed during pathophysiological conditions such as osteoarthritis. Hypertrophic chondrocytes synthesise collagen X and express matrix metalloproteinase 13...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141252

    authors: Filip A,Bianchi A,Mainard D,Lacolley P,Magdalou J,Mercier N

    更新日期:2015-01-01 00:00:00

  • Modelling the mechanical response of skin in vivo using the Bossinesq and the Michell stress functions.

    abstract::Local strains acting across an area of skin loaded uniaxially in vivo are converted to stresses using the standard elastic formulae. The stress values are compared to those obtained using the classical Bossinesq and Michell stress functions. The results indicate that these functions are capable of describing the respo...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Wan Abas WA,Asseli MR

    更新日期:1994-01-01 00:00:00

  • Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses.

    abstract::A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR bi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-151526

    authors: Shi L,Sun Q,He J,Xu H,Liu C,Zhao C,Xu Y,Wu C,Xiang J,Gu D,Long J,Lan H

    更新日期:2015-01-01 00:00:00

  • Automatic recognition of facial movement for paralyzed face.

    abstract::Facial nerve paralysis is a common disease due to nerve damage. Most approaches for evaluating the degree of facial paralysis rely on a set of different facial movements as commanded by doctors. Therefore, automatic recognition of the patterns of facial movement is fundamental to the evaluation of the degree of facial...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-141093

    authors: Wang T,Dong J,Sun X,Zhang S,Wang S

    更新日期:2014-01-01 00:00:00

  • A statistical approach to segmentation of diffusion tensor imaging.

    abstract::The aim of this study is to design a statistical segmentation technique to allow extraction of grey matter, white matter and cerebral spinal fluid volumes from diffusion tensor imaging. Four channel maps of the DTI are used as the input features, which provide more information for brain tissue segmentation compared wi...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130927

    authors: Wen Y,He L

    更新日期:2014-01-01 00:00:00

  • Crystal chemistry of hydroxyapatite deposited on titanium by sputtering technique.

    abstract::Crystalline hydroxyapatite (HA) powder was coated on titanium substrate by radio frequency (RF) magnetron sputtering. The coating was homogeneous thin film and the thickness was 1microm. Crystallinity of the HA coating was low and Ca/P ratio was high as 3.0. Particle sizes were 40 to approximately 100 nm, and the crys...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Ozeki K,Yuhta T,Aoki H,Nishimura I,Fukui Y

    更新日期:2000-01-01 00:00:00

  • IB-LBM simulation on blood cell sorting with a micro-fence structure.

    abstract::A size-based blood cell sorting model with a micro-fence structure is proposed in the frame of immersed boundary and lattice Boltzmann method (IB-LBM). The fluid dynamics is obtained by solving the discrete lattice Boltzmann equation, and the cells motion and deformation are handled by the immersed boundary method. A ...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:10.3233/BME-130833

    authors: Wei Q,Xu YQ,Tian FB,Gao TX,Tang XY,Zu WH

    更新日期:2014-01-01 00:00:00

  • Push-out strength of hydroxyapatite coated by sputtering technique in bone.

    abstract::Hydroxyapatite was coated with 1 microm thickness on titanium columns of a length of 10 mm, an outer diameter of 4.0 mm by radio frequency magnetron sputtering. The hydroxyapatite coating titanium columns were implanted in the diaphysis of femora of 3 adult dogs, and push-out test was carried out after 2, 4 and 12 wee...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Ozeki K,Yuhta T,Aoki H,Nishimura I,Fukui Y

    更新日期:2001-01-01 00:00:00

  • Dentin bonding system. Part I: Literature review.

    abstract::Currently, clinicians face choices of restoration including amalgams (mercury-based, gallium-based alloys, or mercury-free silver-based substitutes), composite resins, ceramics, and gold alloy. In order to choose an appropriate restorative material, many parameters are involved; they include preparation time requireme...

    journal_title:Bio-medical materials and engineering

    pub_type: 历史文章,杂志文章,评审

    doi:

    authors: Miyazaki M,Oshida Y,Xirouchaki L

    更新日期:1996-01-01 00:00:00

  • In vivo rat knee cartilage volume measurement using quantitative high resolution MRI (7 T): feasibility and reproducibility.

    abstract:OBJECTIVES:to assess reliability and reproducibility of quantitative MRI (7 T) in assessing rat femoro-tibial cartilage volume. METHODS:5 healthy rat knees were scanned in vivo using a 7 T experimental imager. Sagittal high resolution 3D Gradient Echo with fat suppression sequences were performed with a dedicated home...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Goebel JC,Bolbos R,Pinzano A,Schaeffer M,Rengle A,Galois L,Etienne S,Netter P,Loeuille D,Beuf O,Gillet P

    更新日期:2008-01-01 00:00:00

  • Use of enzyme-linked immunosorbent assay (ELISA) for detection and quantification of monoclonal antibodies.

    abstract::The thrust of the present work is the demonstration of the feasibility of using an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of a monoclonal antibody human serum. The antibodies used, 7E3 and D3GP3, have been proposed as platelet receptor blockers, being directed specifically again...

    journal_title:Bio-medical materials and engineering

    pub_type: 杂志文章

    doi:

    authors: Lewis G

    更新日期:1994-01-01 00:00:00