Abstract:
:A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.
journal_name
Biomed Mater Engjournal_title
Bio-medical materials and engineeringauthors
Shi L,Sun Q,He J,Xu H,Liu C,Zhao C,Xu Y,Wu C,Xiang J,Gu D,Long J,Lan Hdoi
10.3233/BME-151526subject
Has Abstractpub_date
2015-01-01 00:00:00pages
S2207-16eissn
0959-2989issn
1878-3619pii
BME1526journal_volume
26 Suppl 1pub_type
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journal_title:Bio-medical materials and engineering
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abstract::Intraocular lenses (IOL) made of polymethyl methacrylate (PMMA) lack an important yellowish pigment useful as a filter in the visual process and in the protection of the retina from short wavelength light. It was found that the PMMA used for IOL manufacturing can be tinted by dry exposure to mercury near-UV emitting l...
journal_title:Bio-medical materials and engineering
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journal_title:Bio-medical materials and engineering
pub_type: 临床试验,杂志文章
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journal_title:Bio-medical materials and engineering
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journal_title:Bio-medical materials and engineering
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
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journal_title:Bio-medical materials and engineering
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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更新日期:1995-01-01 00:00:00
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journal_title:Bio-medical materials and engineering
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doi:
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journal_title:Bio-medical materials and engineering
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更新日期:2007-01-01 00:00:00
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