Sterilization of auto-crosslinked hyaluronic acid scaffolds structured in microparticles and sponges.

Abstract:

:This work evaluated the effects of UV irradiation, plasma radiation, steam and 70% ethanol treatments on the sterilization and integrity of auto-crosslinked hyaluronic acid (HA-ACP) scaffolds structured in microparticles and sponges aiming in vivo applications for regenerative medicine of bone tissue. The integrity of the microparticles was characterized by rheological behavior, while for the sponges, it was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effectiveness of the sterilization treatment was verified by the number of microorganism colonies in the samples after the treatments. In conclusion, plasma radiation was the best treatment for the sponges, while steam sterilization in the autoclave at 126°C (1.5 kgf/cm2) for 5 min was the best treatment for the microparticles.

journal_name

Biomed Mater Eng

authors

Shimojo AA,de Souza Brissac IC,Pina LM,Lambert CS,Santana MH

doi

10.3233/BME-151558

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

183-91

issue

3-4

eissn

0959-2989

issn

1878-3619

pii

BME1558

journal_volume

26

pub_type

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