Crosstalk Between Activated Myofibroblasts and β Cells in Injured Mouse Pancreas.


OBJECTIVES:In injury conditions, myofibroblasts are induced to lay down matrix proteins and support the repair process. In this study, we investigated the role of myofibroblasts, particularly stellate cells, in the growth and regeneration of pancreatic β cells. METHODS:We used both in vitro and in vivo approaches to address whether stellate cells may promote the growth of β cells. RESULTS:Our experiments demonstrated that activated stellate cells support the proliferation of β cells in vitro. In vivo, mesenchymals surrounding the pancreatic islets are activated (induced to proliferate) in the islet regeneration model of Pten null mice. These mesenchymals display markers of pancreatic stellate cells, such as desmin and to a lesser extent, smooth muscle actin α. We have shown previously that targeted β-cell deletion of Pten lead to a significant increase in total islet mass. This phenotype was accompanied by an increase in peri-islet mitotic activity, particularly in islets injured by streptozotocin, a β cell-specific toxin. CONCLUSIONS:Together with the in vitro observations, our data, here, suggest that that these mesenchymal cells may support the regeneration of the islets. Identifying how the communication occurs may provide clinically relevant mechanism for inducing β-cell regeneration.






Bayan JA,Peng Z,Zeng N,He L,Chen J,Stiles BL




Has Abstract


2015-10-01 00:00:00














  • Evaluation of the effects of noniodinized and iodinized ionic contrast media and gadoteric acid in acute necrotizing pancreatitis: experimental study in rabbits.

    abstract:OBJECTIVE:To evaluate the effects of iodine contrast media and gadoteric acid in acute necrotizing pancreatitis. METHODS:Fifty rabbits were distributed in 5 groups: 10 rabbits were assigned in the control group (group 5) and 40 rabbits were assigned in the pancreatitis group, wherein acute necrotizing pancreatitis was...


    pub_type: 杂志文章


    authors: Apodaca-Torrez FR,Goldenberg A,Lobo EJ,Farah JF,Triviño T,Montero EF,Schraibman V,Benvenuto RB,Artigiani Neto R

    更新日期:2007-11-01 00:00:00

  • Transplantation of rat islets transduced with human heme oxygenase-1 gene using adenovirus vector.

    abstract:OBJECTIVE:To investigate whether human heme oxygenase-1 (HO-1) gene has protective action on islets cultured in vitro, and to explore whether transduction of HO-1 gene to donor islets could enhance engrafted islets survival and suppress local lymphocytic infiltration in islet grafts. METHODS:Newly isolated rat islets ...


    pub_type: 杂志文章


    authors: Li Y,Li G,Dong W,Chen J,Lu D,Tan J

    更新日期:2006-10-01 00:00:00

  • External validation of 2 prognostic indices for patients with advanced pancreatic cancer treated with first-line therapy.

    abstract:OBJECTIVES:The objective of this study was to perform an external validation of 2 Asian prognostic indices for patients with advanced pancreatic cancer. METHODS:A score was calculated in patients treated with frontline therapy derived from the factors Eastern Cooperative Oncology Group Performance Status, localization...


    pub_type: 杂志文章


    authors: Haas M,Laubender RP,Klose C,Schulz C,Mansmann U,Boeck S,Heinemann V

    更新日期:2012-07-01 00:00:00

  • Personalized Pancreatic Cancer Management: A Systematic Review of How Machine Learning Is Supporting Decision-making.

    abstract::This review critically analyzes how machine learning is being used to support clinical decision-making in the management of potentially resectable pancreatic cancer. Following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines, electronic searches of MEDLINE, Embase, PubMed, and Coc...


    pub_type: 杂志文章


    authors: Bradley A,van der Meer R,McKay C

    更新日期:2019-05-01 00:00:00

  • Issues in clinical islet transplantation.

    abstract::The Second International Islet Transplantation Symposium was held at UCLA in January, 1993. The symposium, sponsored by the Jerry's Foundation for Diabetes Research was focused on the issues in clinical islet transplantation. The leaders from each of the clinically active programs around the world were invited to disc...


    pub_type: 杂志文章,评审


    authors: Brunicardi FC,Mullen Y

    更新日期:1994-05-01 00:00:00

  • Pancreatic Cysts - Part 1: Using the American Gastroenterological Association Guidelines for the Management of Pancreatic Cysts-A Practical Approach.

    abstract::The recent publication of evidence-based guidelines by the American Gastroenterological Association for the management of the incidental pancreatic cyst has stimulated considerable discussion among clinicians and researchers. The guidelines examined the evidence in an accompanying technical review defining current dia...


    pub_type: 杂志文章,评审


    authors: Scheiman JM

    更新日期:2017-07-01 00:00:00

  • Diagnostic imaging for pancreatic cancer: computed tomography, magnetic resonance imaging, and positron emission tomography.

    abstract::Computed tomography (CT), magnetic resonance imaging (MRI), and positron emission tomography (PET) are sophisticated modalities typically used in the second-line diagnosis following routine clinical practice. Among them, CT is regarded as the standard imaging in diagnosing pancreatic cancer at present in Japan due to ...


    pub_type: 杂志文章


    authors: Saisho H,Yamaguchi T

    更新日期:2004-04-01 00:00:00

  • Acute postprandial gastrointestinal and metabolic effects of wheat amylase inhibitor (WAI) in normal, obese, and diabetic humans.

    abstract::Amylase inhibition has gastrointestinal and metabolic effects that may aid in the treatment of diabetes and obesity. We tested whether 4 g of a commercially available wheat amylase inhibitor (WAI) affected postprandial carbohydrate (CHO) absorption and plasma glucose or hormones. Twelve persons (four lean and four obe...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Lankisch M,Layer P,Rizza RA,DiMagno EP

    更新日期:1998-08-01 00:00:00

  • Endoscopic ultrasound-guided pancreatic fiducial placement: how important is ideal fiducial geometry?

    abstract:OBJECTIVE:Image-guided radiation therapy allows precise tumor targeting using real-time tracking of radiopaque fiducial markers. To enable appropriate tracking, it is recommended to place fiducials with "ideal fiducial geometry" (IFG). Our objectives were to determine the proportion of patients in whom IFG can be achie...


    pub_type: 杂志文章


    authors: Majumder S,Berzin TM,Mahadevan A,Pawa R,Ellsmere J,Sepe PS,Larosa SA,Pleskow DK,Chuttani R,Sawhney MS

    更新日期:2013-05-01 00:00:00

  • Identification of thioredoxin-interacting protein 1 as a hypoxia-inducible factor 1alpha-induced gene in pancreatic cancer.

    abstract:OBJECTIVE:To investigate the expression of thioredoxin-interacting protein (TXNIP) during hypoxia and its dependency on hypoxia-inducible factor 1alpha (HIF-1alpha) in pancreatic cancer cell lines. METHODS:MiaPaCa-2 pancreatic cancer cells were transiently transfected with siRNA to HIF-1alpha and TXNIP protein measure...


    pub_type: 杂志文章


    authors: Baker AF,Koh MY,Williams RR,James B,Wang H,Tate WR,Gallegos A,Von Hoff DD,Han H,Powis G

    更新日期:2008-03-01 00:00:00

  • Reported findings on endoscopic ultrasound examinations for chronic pancreatitis: toward establishing an endoscopic ultrasound quality benchmark.

    abstract:OBJECTIVES:Endoscopic ultrasound (EUS) quality benchmarks for pancreatic disease previously focused on maintaining thresholds of diagnostic accuracy for fine-needle aspiration and measuring complications. We aimed to evaluate quality indicators when performing EUS specifically for the diagnosis of chronic pancreatitis ...


    pub_type: 杂志文章


    authors: Gardner TB,Taylor DJ,Gordon SR

    更新日期:2014-01-01 00:00:00

  • Clinicopathologic Features and Germline Sequence Variants in Young Patients (≤40 Years Old) With Pancreatic Ductal Adenocarcinoma.

    abstract:OBJECTIVES:The median age of patients with pancreatic ductal adenocarcinoma (PDAC) is approximately 70 years, and PDAC rarely affects individuals younger than 40 years. Here, we investigated clinicopathologic features and genetic background of PDAC occurring in young patients (age ≤ 40 years) to determine whether any d...


    pub_type: 杂志文章


    authors: Ohmoto A,Yachida S,Kubo E,Takai E,Suzuki M,Shimada K,Okusaka T,Morizane C

    更新日期:2016-08-01 00:00:00

  • Exogenous insulin does not influence CCK- and meal-stimulated pancreatic secretion.

    abstract::Although previous reports suggest interactions between the endocrine and the exocrine pancreas, insulin's effect on pancreatic exocrine function remains unclear. Chronic pancreatic fistulae were created in five dogs; these animals were studied using the euglycemic, hyperinsulinemic clamp technique. After a 30-min unst...


    pub_type: 杂志文章


    authors: Berry SM,Fink AS

    更新日期:1996-05-01 00:00:00

  • Chronic Asymptomatic Pancreatic Hyperenzymemia: A Long-term Follow-up.

    abstract:OBJECTIVES:Chronic asymptomatic pancreatic hyperenzymemia (CAPH) was described as a benign disease. However, we already described clinically relevant findings requiring surgery or follow-up in half of the subjects. The aim of this study was to evaluate the long-term outcome of CAPH in terms of symptoms and evolution to...


    pub_type: 杂志文章


    authors: Amodio A,De Marchi G,Granato A,de Pretis N,Gabbrielli A,Manfredi R,Pozzi Mucelli R,Frulloni L

    更新日期:2019-04-01 00:00:00

  • Endoscopic stent therapy for patients with chronic pancreatitis: results from a prospective follow-up study.

    abstract:OBJECTIVES:Obstruction of the pancreatic duct is a common feature of chronic pancreatitis and often requires interventional therapy. The current prospective study investigated clinical success in 19 patients after initial endoscopic retrograde pancreaticography and relapse rates during a 2-year follow-up period. METHO...


    pub_type: 杂志文章


    authors: Weber A,Schneider J,Neu B,Meining A,Born P,Schmid RM,Prinz C

    更新日期:2007-04-01 00:00:00

  • Energy metabolism in mouse pancreas in response to different dosages of a CCK analogue.

    abstract::Stimulation of the exocrine pancreas with cholecystokinin analogues leads to a variety of intraacinar processes, many coupled to energy consumption. It was hypothesized that extensive ATP depletion could play a role in the pathophysiology of acute pancreatitis, especially in the hyperstimulation (cerulein) model. Mice...


    pub_type: 杂志文章


    authors: Lüthen RE,Niederau C,Ferrell LD,Grendell JH

    更新日期:1995-08-01 00:00:00

  • Effect and cost of treatment for acute pancreatitis with or without gabexate mesylate: a propensity score analysis using a nationwide administrative database.

    abstract:OBJECTIVES:Despite a lack of evidence, gabexate mesylate (GM) is routinely used for the treatment of acute pancreatitis (AP) in some countries. The present study examined the effect and cost of GM for AP treatment using the Japanese Diagnosis Procedure Combination database. METHODS:We performed a propensity score anal...


    pub_type: 杂志文章


    authors: Yasunaga H,Horiguchi H,Hashimoto H,Matsuda S,Fushimi K

    更新日期:2013-03-01 00:00:00

  • Expression of the beacon gene in the rat pancreatic islets: opposite effects of beacon (47-73) protein (ubiquitin-like protein 5) on insulin secretion in vivo and insulin release by isolated islets.

    abstract:OBJECTIVE:Beacon gene expression is elevated in the hypothalamus of the Israeli sand rat (Psammomys obesus), an animal that is used as a polygenic animal model of obesity and NIDDM. We performed studies aimed at investigating the expression of beacon mRNA and protein in pancreatic islets of the rat and the possible bea...


    pub_type: 杂志文章


    authors: Nowak KW,Ruciński M,Kaczmarek P,Szkudelski T,Malendowicz LK

    更新日期:2004-08-01 00:00:00

  • Effect of chronic amylase inhibition on pancreatic growth and acinar cell secretory function in rats.

    abstract::Wheat amylase inhibitor (WAI) was given to growing rats to determine whether chronic inhibition of intraluminal amylase activity alters pancreatic growth, pancreatic enzyme composition, and secretory responsiveness to cholecystokinin octapeptide (CCK-OP) and carbachol. For 21 days 13 rats were fed amylase inhibitor (A...


    pub_type: 杂志文章


    authors: Kataoka K,DiMagno EP

    更新日期:1998-07-01 00:00:00

  • Reduced expression of PDX-1 is associated with decreased beta cell function in chronic pancreatitis.

    abstract:OBJECTIVES:The present study was conducted to monitor the expression of pancreas and duodenal homeobox gene (PDX-1) for assessing beta-cell function in islets from patients with chronic pancreatitis (CP). METHODS:Islets isolated from the pancreata of 40 surgical patients categorized as control group, patients with mil...


    pub_type: 杂志文章


    authors: Mitnala S,Pondugala PK,Guduru VR,Rabella P,Thiyyari J,Chivukula S,Boddupalli S,Hardikar AA,Reddy DN

    更新日期:2010-08-01 00:00:00

  • Cholecystokinin stimulates a specific ribosomal S6 kinase in rat pancreatic acini.

    abstract::Stimulation of intact rat pancreatic acini with cholecystokinin (CCK) enhances the phosphorylation of the ribosomal protein S6 in a dose-dependent manner with half maximal stimulation at 40 pM and maximal stimulation at 1 nM CCK octapeptide. Soluble cellular extracts contained S6 kinase activity assayed using purified...


    pub_type: 杂志文章


    authors: Sung CK,Williams JA

    更新日期:1990-11-01 00:00:00

  • Is Multifocality an Indicator of Aggressive Behavior in Small Bowel Neuroendocrine Tumors?

    abstract:OBJECTIVES:Many patients with small bowel neuroendocrine tumors (SBNETs) have multifocal tumors (MFTs), but the frequency of MFTs has varied widely across SBNET studies. It is also unclear whether patients with MFTs have more advanced disease or worse clinical course than do those with unifocal SBNETs. We set out to de...


    pub_type: 杂志文章


    authors: Choi AB,Maxwell JE,Keck KJ,Bellizzi AJ,Dillon JS,OʼDorisio TM,Howe JR

    更新日期:2017-10-01 00:00:00

  • Involvement of endogenous prostaglandins in pancreatic endocrine and exocrine secretion in dog pancreas.

    abstract::The involvement of endogenous prostaglandins (PGs) in pancreatic endocrine and exocrine secretion was investigated, using the isolated and perfused dog pancreas. Spontaneous production of both PGE2 and 6-keto-PGF1 alpha was recorded in venous effluent. Prostaglandin production increased following stimulation with both...


    pub_type: 杂志文章


    authors: Kuroda T,Sodeyama H,Hanazaki K,Horigome N,Kajikawa S,Yamagishi K,Horiuchi A,Iwatsuki K,Chiba S,Homma T

    更新日期:1989-01-01 00:00:00

  • Pancreatic hypersecretion in the jejunal-bypass rat.

    abstract::We diverted bile and pancreatic juice from jejunal blind loops of different lengths in conscious rats to see if the pancreatic secretory response was dependent on the length of the jejunal blind loop. Short-term bile and pancreatic juice diversion from a short jejunal blind loop, representing only 8-10% of the total l...


    pub_type: 杂志文章


    authors: Spannagel AW,Green GM

    更新日期:1999-01-01 00:00:00

  • Clinical Utility of Histological and Radiological Evaluations of Tumor Necrosis for Predicting Prognosis in Pancreatic Cancer.

    abstract:OBJECTIVES:Tumor necrosis is often found in pancreatic ductal adenocarcinoma (PDAC). Objective histological assessment and adequate radiological detection of necrosis could be used as biomarkers for therapeutic decision. However, standardized clinical utility of necrosis remains unknown. Here, we aimed to determine the...


    pub_type: 杂志文章


    authors: Kudo M,Kobayashi T,Gotohda N,Konishi M,Takahashi S,Kobayashi S,Sugimoto M,Okubo S,Martin J,Cabral H,Ishii G,Kojima M

    更新日期:2020-05-01 00:00:00

  • Engulfment of gram-positive bacteria by pancreatic stellate cells in pancreatic fibrosis.

    abstract:OBJECTIVES:We previously reported the finding that pancreatic stellate cells (PSCs) have a phagocytic function. The aim of the present study was to investigate whether engulfment of gram-positive bacteria by PSCs plays any role in the pathogenesis of pancreatic fibrosis. METHODS:Rat PSCs were cultured with lipoteichoi...


    pub_type: 杂志文章


    authors: Morishita K,Shimizu K,Haruta I,Kawamura S,Kobayashi M,Shiratori K

    更新日期:2010-10-01 00:00:00

  • PANC-1 pancreatic cancer cell growth inhibited by cucurmosin alone and in combination with an epidermal growth factor receptor-targeted drug.

    abstract:OBJECTIVES:To investigate the inhibition of PANC-1 pancreatic cancer cell growth by cucurmosin (CUS) and its possible mechanism. METHODS:We observed the inhibition of PANC-1 cell growth by sulforhodamine B and colony-forming experiments in vitro and established nonobese diabetic/severe combined immunodeficiency mouse ...


    pub_type: 杂志文章


    authors: Wang C,Yang A,Zhang B,Yin Q,Huang H,Chen M,Xie J

    更新日期:2014-03-01 00:00:00

  • Pancreatic hypertrophy in Ki-ras-induced actin-interacting protein gene knockout mice.

    abstract:OBJECTIVES:Pancreatic functions were determined in a Ki-ras-induced actin-interacting protein (KRAP)-deficient (-/-) mouse mutant. METHODS:Pancreatic enzyme, protein, and DNA contents were measured, and histological examinations were conducted. The mixture of bile-pancreatic juice was collected, and amylase and bile a...


    pub_type: 杂志文章


    authors: Miyasaka K,Fujimoto T,Kawanami T,Takiguchi S,Jimi A,Funakoshi A,Shirasawa S

    更新日期:2011-01-01 00:00:00

  • Binge drinking aggravates the outcomes of first-attack severe acute pancreatitis.

    abstract:OBJECTIVES:To study the association of binge drinking and the outcomes of severe acute pancreatitis (SAP). METHODS:This retrospective study included 347 patients with first-attack SAP from January 2001 to February 2004. On the basis of the history of binge drinking or not, the patients were divided into the alcohol (n...


    pub_type: 杂志文章


    authors: Deng L,Xue P,Huang L,Yang X,Wan M,Xia Q

    更新日期:2010-03-01 00:00:00

  • Abdominal pain in patients with resectable pancreatic cancer with reference to clinicopathologic findings.

    abstract::Abdominal and/or back pain is one of the most common symptoms in patients with pancreatic cancer. However, the cause of the pain and the clinicopathologic features of patients with pain have not been fully elucidated. We retrospectively determined the factors related to preoperative abdominal and/or back pain in 95 pa...


    pub_type: 杂志文章


    authors: Okusaka T,Okada S,Ueno H,Ikeda M,Shimada K,Yamamoto J,Kosuge T,Yamasaki S,Fukushima N,Sakamoto M

    更新日期:2001-04-01 00:00:00