Abstract:
:As new killer-cell immunoglobulin-like receptor (KIR) alleles are discovered, a challenge in KIR typing is maintaining sensitivity and specificity. A single nucleotide polymorphism assay can be used to type functional KIR2DL1 alleles. We improved recently on the earlier method by using a higher-specificity assay. The major modifications include the development of sequence-specific primers to selectively amplify the transmembrane domain of all known KIR2DL1 alleles via polymerase chain reaction with sequence-specific primers (PCR-SSP), and using the PCR products as the template for a revised KIR2DL1 functional allele-typing assay. This modified method allows high-throughput typing with high specificity.
journal_name
Lab Medjournal_title
Laboratory medicineauthors
Bari R,Schell S,Tuggle M,Leung Wdoi
10.1309/LMMN1K6SBRJCGD9Jsubject
Has Abstractpub_date
2015-07-01 00:00:00pages
207-13issue
3eissn
0007-5027issn
1943-7730pii
46/3/207journal_volume
46pub_type
杂志文章abstract:OBJECTIVE:Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS:Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacua...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lm2xcv5sqml1ontm
更新日期:2014-04-01 00:00:00
abstract::Waldenström macroglobulinemia (WM) is a form of lymphoplasmacytic lymphoma that can cause hyperviscosity syndrome due to unchecked monoclonal antibody production. Some patients are also found to have associated cryoglobulinemia, which can cause systemic complications including vasculitis, renal disease, and pulmonary ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmy073
更新日期:2019-07-16 00:00:00
abstract:Background:Anti-phospholipase A2 receptor (anti-PLA2R) is a promising biomarker for diagnosis, activity evaluation, therapy monitoring, and prognostic estimation of primary membranous neuropathy (pMN). Difference in methodology may be one cause of the discrepancy in anti-PLA2R-positive percentages in reported studies. ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmy016
更新日期:2018-10-11 00:00:00
abstract:BACKGROUND:Research in several professional fields has demonstrated that delays (time lapse) in taking certification examinations may result in poorer performance by examinees. Thirteen states and/or territories require licensure for laboratory personnel. A core component of licensure is passing a certification exam. A...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMNM534LIACPBZWH
更新日期:2015-07-01 00:00:00
abstract::Cell-derived microparticles (MPs) are small fragments released from various cells when they are activated or undergo apoptosis. In the field of transfusion medicine, a number of studies have documented increased levels of MPs in blood products, which have been associated with multiple factors, including donor variabil...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz100
更新日期:2020-09-01 00:00:00
abstract::Rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. Isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (HIV) associated immunosuppression, who have catheter or medical de...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw019
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:Knowledge of transfusion medicine is the key element of better transfusion practices. This deficit observed at the blood bank end on daily basis exposing the patients for redundant risk. We assessed the knowledge of transfusion medicine among post intern doctors. OBJECTIVE:To assess the knowledge of transfu...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw056
更新日期:2017-02-01 00:00:00
abstract::Hereditary hemochromatosis (HH) is an autosomal recessive disorder of iron metabolism characterized by increased iron absorption and tissue deposition. Three loss-of-function mutations in the hemochromatosis gene (HFE), namely, C282Y (c.845G>A), H63D (c.187C>G), and S65C (c.193A>T), account for the vast majority of HH...
journal_title:Laboratory medicine
pub_type: 杂志文章,评审
doi:10.1093/labmed/lmy065
更新日期:2019-04-08 00:00:00
abstract::Rivaroxaban (Xarelto; Johnson & Johnson Services, Inc) is a direct oral anticoagulant (DOAC) that works by directly inhibiting the active site of factor Xa (FXa). Rivaroxaban is metabolized and cleared via the kidney and liver. The results of various studies have shown that patients with severe renal impairment should...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz044
更新日期:2020-03-10 00:00:00
abstract:OBJECTIVE:Examination of urine sediment is crucial in acute kidney injury (AKI). In such renal injury, tubular epithelial cells, epithelial cell casts, and dysmorphic red cells may provide clues to etiology. The aim of this study was to compare automated urinalysis findings with manual microscopic analysis in AKI. MET...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmaa069
更新日期:2020-10-03 00:00:00
abstract:BACKGROUND:Oxidative stress has been associated with the progression of atherosclerosis and activation of genes that lead to increased deposition of proteins in the extracellular matrix. Bone sialoprotein (BSP) and osteonectin are proteins involved in the initiation and progression of vascular calcification. OBJECTIVE...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMUJWVQFW6CJMSOQ
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:Consanguineous marriage, a common practice in South India, increase the incidence of autosomal recessive diseases such as nonsyndromic hearing loss (NSHL) in offspring. This trend was noted in the children with hearing impairment (HI) who received cochlear implants (CI) at our University hospital in Porur, C...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz027
更新日期:2020-01-02 00:00:00
abstract:OBJECTIVES:To determine the effect of a modified request form on the total use of γ-glutamyl transferase (GGT) usage and the ratio of inappropriate testing. METHODS:We modified a test request form by moving GGT from the "Liver profile" to the "Other" section. The criteria for appropriate GGT ordering were developed ba...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LM7E5LG6PWJYEFUJ
更新日期:2015-07-01 00:00:00
abstract:OBJECTIVE:To determine whether K2-ethylenediaminetetraacetic acid (EDTA) and K3-EDTA Greiner tubes could be used interchangeably for glycosylated hemoglobin, type A1C (HbA1c) measurement via the Abbott Laboratories ARCHITECT chemiluminescent microparticle HbA1c assay on the ARCHITECT i2000SR immunoanalyzer at our unive...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmv016
更新日期:2016-02-01 00:00:00
abstract:OBJECTIVE:Inflammation of the small intestine may occur in type 2 diabetes. This study aimed to investigate whether ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) were altered in chronic inflammation of the small intestine of type 2 diabetic rats. METHODS:Thirty-two male Sprague-Dawley rats were used. Eigh...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lmo485spyxbqanxj
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To evaluate and compare the performances of the automated urinalysis devices UX-2000 and Cobas 6500. METHOD:A total of 258 urine specimens were collected from the routine specimen workload. We analyzed all specimens on both automated instruments and recorded the turnaround time from each method. Physical, ch...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw002
更新日期:2016-05-01 00:00:00
abstract:Background:When unpredicted surgical blood loss occurs, it is essential that blood arrive from the blood bank in a timely manner. Objective:To evaluate the turnaround time (TAT) for red blood cells (RBCs) from the blood bank to the operating suite. Cases with delayed TATs were further analyzed to determine potential c...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx016
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:Panels of clinical laboratory testing may generate "incidental" critical values from unordered parameters. Existing regulations do not clearly delineate guidelines for handling incidental critical values. The objective of this study was to examine the patterns and clinical utility of incidental critical value...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw044
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. METHODS:...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lms0wn7k7ukzcpmi
更新日期:2014-01-01 00:00:00
abstract::We report a case of a false-positive HIV result in an apparently healthy pregnant woman. Since no prenatal HIV testing had been performed, we screened for HIV reactivity utilizing the Architect HIV-Ag/Ab Combo assay. Results obtained were inconsistent in that they were repeatedly HIV reactive on a single serum sample ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMAAGVXK05LUWOQN
更新日期:2014-07-01 00:00:00
abstract::Primary amoebic meningoencephalitis (PAM) is a rare and almost always fatal disease that is caused by Naegleria fowleri, a freshwater thermophilic amoeba. Our case involves an adolescent female who presented with fever of unknown origin. A lumbar puncture was performed, and the Wright-Giemsa and Gram stained cerebrosp...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw008
更新日期:2016-05-01 00:00:00
abstract:PATIENT:78-year-old African-American man. PAST MEDICAL HISTORY:Chronic lymphocytic leukemia first diagnosed in 2003, with a subsequent relapse in 2006 and another in 2010. HISTORY OF PRESENT ILLNESS:In late 2011, the patient was admitted to the hospital for cholelithiasis, at which time his treating physician inciden...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/LMLWQDB2Q6LS7VQG
更新日期:2014-07-01 00:00:00
abstract:Background:Early access for routine testing with the Alinity c clinical chemistry system (Abbot Laboratories) presented the opportunity to characterize the analytical performance of multiple analytes across clinical laboratories in Europe. Methods:A total of 8 laboratories from 7 European countries evaluated 10 high-v...
journal_title:Laboratory medicine
pub_type: 杂志文章,多中心研究
doi:10.1093/labmed/lmy053
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS:We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw027
更新日期:2016-11-01 00:00:00
abstract:Background:Interest in vitamin D has increased during the past 2 decades, with a corresponding increase in laboratory testing of 25-hydroxyvitamin D [25(OH)D]. The vast majority of specimens tested display normal or deficient levels of 25(OH)D; concentrations rarely fall in the potentially toxic range. Methods:We perf...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx077
更新日期:2018-03-21 00:00:00
abstract:BACKGROUND:The University of Maryland Medical System, like all other medical systems, is faced with providing the highest quality care in an environment of increased demands and competition for resources. Some responses to this pressure include improved integration, coordination, and efficiency. Laboratory integration ...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmw051
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:The production of 2 monoclonal proteins characterizes biclonal gammopathic manifestations (BGMs). The available medical literature from India is chiefly restricted to case reports. OBJECTIVE:To study the incidence of BGMs in a tertiary care center in Chandigarh, India, during a 4-year period. We evaluated t...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz006
更新日期:2019-10-10 00:00:00
abstract:Objective:To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)- and nicotinamide adenine dinucleotide phosphate (NADPH)-consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods:We measured ammonia concentrations using GLDH and NADH...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx053
更新日期:2017-11-08 00:00:00
abstract:OBJECTIVE:To identify the osteoprotegerin (OPG) correlates with antiphospholipid syndrome (APS) parameters. METHODS:Our cohort included 40 patients with primary APS disease associated with systemic lupus erythematosus (SLE) (mean age, 43.7 years; 87% female). Data on cardiovascular risk factors and specific clinical e...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmz051
更新日期:2020-03-10 00:00:00
abstract::Caspases play a vital role during apoptosis. In addition to apoptosis, caspases play a role in cytokine gene induction and work to inhibit apoptosis. In order for individuals to thrive with useful tissue growth, the rate of cell growth and division must surpass the rate of cell division. It is well established that ex...
journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1093/labmed/lmx086
更新日期:2018-03-21 00:00:00