The Effectiveness of Temperature Versus Aprotinin in Maintaining the Preanalytical Stability of Adrenocorticotrophin.

Abstract:

OBJECTIVE:To investigate the effect of temperature and protease inhibitor on adrenocorticotrophin (ACTH) stability. METHODS:We collected blood specimens from 10 donors into K3 EDTA (ethylenediaminetetraacetic acid) and K3 EDTA + aprotinin tubes. Plasma specimens were held at room temperature and at 2 °C to 8[Formula: see text] for 0, 2, 4, 8, 24, and 72 hours. ACTH concentrations in 4 storage conditions were compared with repeated-measures analysis of variance (ANOVA) at each time point. RESULTS:ACTH concentrations substantially decreased after a 72-hour time period. At the 72nd hour, standard EDTA tubes and EDTA + aprotinin tubes stored at 4[Formula: see text] showed the optimal stability. EDTA + aprotinin tubes stored at room temperature performed more strongly than standard EDTA tubes stored at room temperature. CONCLUSION:Our results show that refrigerated storage is more effective than protease inhibitor addition to maintain ACTH stability. We recommend using aprotinin tubes, especially for specimens standing at room temperature.

journal_name

Lab Med

journal_title

Laboratory medicine

authors

Turkon H,Toprak B,Yalcin H,Colak A,Ozturk N

doi

10.1093/labmed/lmw027

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

279-282

issue

4

eissn

0007-5027

issn

1943-7730

pii

lmw027

journal_volume

47

pub_type

杂志文章
  • What Clinical Laboratorians Should Do in Response to Extremely Low Hemoglobin A1c Results.

    abstract::Extremely low hemoglobin A1c (HbA1c) results below reference range are rare, and the causes and clinical implications associated with low HbA1c results are not well understood among clinical laboratorians. A case of extremely low HbA1c results was reported, in which liver cirrhosis, subacute hemorrhage and recent tran...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw050

    authors: Wang P

    更新日期:2017-02-01 00:00:00

  • Blood collection from intravenous lines: is one drawing site better than others?

    abstract:OBJECTIVE:Establish whether hemolysis in samples collected from intravenous lines is influenced by catheterization site. METHODS:Blood was collected from all patients (67 total) admitted to the emergency department the same morning, through a 20-gauge catheter placed in a vein of the upper limb directly into an evacua...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/lm2xcv5sqml1ontm

    authors: Lippi G,Avanzini P,Aloe R,Cervellin G

    更新日期:2014-04-01 00:00:00

  • Reproducibility of TEG Parameters in Stable Cirrhotics.

    abstract:Background:Thromboelastography (TEG) has become the standard of care in liver-transplant surgery to identify real-time abnormalities in the coagulation cascade. To our knowledge, no studies have been performed to measure the intrasubject reproducibility of TEG parameters in cirrhotic patients. Objective:To perform a v...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx041

    authors: George G,Manatasahit W,Balasubramanian M,Navarro V

    更新日期:2018-07-05 00:00:00

  • Deproteinization is Necessary for the Accurate Determination of Ammonia Levels by Glutamate Dehydrogenase Assay in Blood Plasma From Subjects With Liver Injury.

    abstract:Objective:To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)- and nicotinamide adenine dinucleotide phosphate (NADPH)-consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods:We measured ammonia concentrations using GLDH and NADH...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx053

    authors: Vodenicarovova M,Skalska H,Holecek M

    更新日期:2017-11-08 00:00:00

  • Following the Rules Set by Accreditation Agencies and Governing Bodies to Maintain In-Compliance Status: Applying Critical Thinking Skills When Evaluating the Need for Change in the Clinical Laboratory.

    abstract::Maintaining an in-compliance clinical laboratory takes continuous awareness and review of standards, regulations, and best practices. A strong quality assurance program and well informed leaders who maintain professional networks can aid in this necessary task. This article will discuss a process that laboratories can...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw007

    authors: Byrne KM,Levy KY,Reese EM

    更新日期:2016-05-01 00:00:00

  • Potential Use of Antigen-Based Rapid Test for SARS-CoV-2 in Respiratory Specimens in Low-Resource Settings in Egypt for Symptomatic Patients and High-Risk Contacts.

    abstract:OBJECTIVE:Because of the rapidly emerging SARS-CoV-2 pandemic and its wide public health challenges, rapid diagnosis is essential to decrease the spread. Antigen-based rapid detection tests are available; however, insufficient data about their performance are available. METHODS:The lateral-flow immunochromatographic B...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmaa104

    authors: Abdelrazik AM,Elshafie SM,Abdelaziz HM

    更新日期:2020-12-07 00:00:00

  • Performance Characteristics and Clinical Value of the Lipoprotein-Associated Phospholipase A2 by an Enzymatic Kinetic Method.

    abstract:OBJECTIVE:To analyze the performance characteristics, stability, and clinical value of lipoprotein-associated phospholipase A2 (Lp-PLA2) using an enzymatic kinetic method. METHODS:The performance characteristics included reference intervals, precision, and accuracy. We assessed Lp-PLA2 stability by comparing Lp-PLA2 c...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmy086

    authors: Lu M,Lv L

    更新日期:2019-07-16 00:00:00

  • ABCA1 and ABCG1 expression in the small intestine of type 2 diabetic rats.

    abstract:OBJECTIVE:Inflammation of the small intestine may occur in type 2 diabetes. This study aimed to investigate whether ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) were altered in chronic inflammation of the small intestine of type 2 diabetic rats. METHODS:Thirty-two male Sprague-Dawley rats were used. Eigh...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/lmo485spyxbqanxj

    authors: Lou J,Zhou H,Li C,Hu L,Lu X,Li J,Yao H,Li W,Zhang X,Xu M

    更新日期:2014-01-01 00:00:00

  • Hypoxia-Induced TGFBI as a Serum Biomarker for Laboratory Diagnosis and Prognosis in Patients with Pancreatic Ductal Adenocarcinoma.

    abstract:OBJECTIVE:To explore novel biomarkers for patients with pancreatic ductal adenocarcinoma (PDAC), from the perspective of tumor hypoxia. METHODS:We screened 29 differentially expressed and hypoxia-upregulated genes from the Oncomine database. A total of 12 secretory proteins that interact with hypoxia-inducible factor ...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz063

    authors: Yang L,Cui R,Li Y,Liang K,Ni M,Gu Y

    更新日期:2020-07-08 00:00:00

  • An Analysis of Multirules for Monitoring Assay Quality Control.

    abstract:BACKGROUND:Multirules are often employed to monitor quality control (QC). The performance of multirules is usually determined by simulation and is difficult to predict. Previous studies have not provided computer code that would enable one to experiment with multirules. It would be helpful for analysts to have computer...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz038

    authors: Walker BS,Pearson LN,Schmidt RL

    更新日期:2020-01-02 00:00:00

  • Prevalence and Clinical Utility of "Incidental" Critical Values Resulting From Critical Care Laboratory Testing.

    abstract:OBJECTIVE:Panels of clinical laboratory testing may generate "incidental" critical values from unordered parameters. Existing regulations do not clearly delineate guidelines for handling incidental critical values. The objective of this study was to examine the patterns and clinical utility of incidental critical value...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw044

    authors: Grieme CV,Voss DR,Olson KE,Davis SR,Kulhavy J,Krasowski MD

    更新日期:2016-11-01 00:00:00

  • Vitamin D Toxicity: A 16-Year Retrospective Study at an Academic Medical Center.

    abstract:Background:Interest in vitamin D has increased during the past 2 decades, with a corresponding increase in laboratory testing of 25-hydroxyvitamin D [25(OH)D]. The vast majority of specimens tested display normal or deficient levels of 25(OH)D; concentrations rarely fall in the potentially toxic range. Methods:We perf...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx077

    authors: Lee JP,Tansey M,Jetton JG,Krasowski MD

    更新日期:2018-03-21 00:00:00

  • Accidental diagnosis: Aeromonas infection leading to discovery of acute myeloid leukemia with meylodysplastic syndrome.

    abstract:PATIENT:47-year-old African American man. CHIEF COMPLAINT:Initially, serious finger abscess. The patient reported severe pain that was localized to that finger only. HISTORY OF PRESENT ILLNESS:His finger had become sore and then progressively more painful and swollen. PAST MEDICAL HISTORY:A few weeks earlier, the pa...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMX711TANCVDHZJJ

    authors: Rausch S

    更新日期:2014-07-01 00:00:00

  • Detection of Platelet Clumps on Peripheral Blood Smears by CellaVision DM96 System and Microscopic Review.

    abstract:OBJECTIVE:To determine and optimize the sensitivity of the CellaVision DM96 automated image-analysis system in detecting platelet (PLT) clumps on blood smears and to assess the reliability of the traditional laboratory practice of examining only the feather edge of the smear for PLT clumps. METHODS:We processed 102 bl...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LM604RQVKVLRFXOR

    authors: Gulati G,Uppal G,Florea AD,Gong J

    更新日期:2014-10-01 00:00:00

  • Mesenchymal Stem Cells in COVID-19: A Journey from Bench to Bedside.

    abstract::The COVID-19 pandemic has led to a major setback in both the health and economic sectors across the globe. The scale of the problem is enormous because we still do not have any specific anti-SARS-CoV-2 antiviral agent or vaccine. The human immune system has never been exposed to this novel virus, so the viral interact...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,评审

    doi:10.1093/labmed/lmaa049

    authors: Sahu KK,Siddiqui AD,Cerny J

    更新日期:2021-01-04 00:00:00

  • Establishing the Cause of Anemia in a Premature Newborn Infant.

    abstract::The three major causes of anemia in neonates are blood loss, decreased red blood cell production, and increased degradation of erythrocytes. Establishing the cause of anemia in a neonate born prematurely can be challenging. Clinically, fetomaternal hemorrhage (FMH) can be difficult to diagnose-the condition often pres...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmy026

    authors: den Besten G,van der Weide K,Schuerman FABA,Michael Cotten C,Rondeel JMM

    更新日期:2018-07-05 00:00:00

  • Drug-induced autoimmune hemolytic anemia in a 78-year-old African-American man with chronic lymphocytic leukemia.

    abstract:PATIENT:78-year-old African-American man. PAST MEDICAL HISTORY:Chronic lymphocytic leukemia first diagnosed in 2003, with a subsequent relapse in 2006 and another in 2010. HISTORY OF PRESENT ILLNESS:In late 2011, the patient was admitted to the hospital for cholelithiasis, at which time his treating physician inciden...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMLWQDB2Q6LS7VQG

    authors: Sartori A,Staley B,Skipper A

    更新日期:2014-07-01 00:00:00

  • Persistent Rivaroxaban Effect Due to Impaired Renal Clearance and Medication Effects.

    abstract::Rivaroxaban (Xarelto; Johnson & Johnson Services, Inc) is a direct oral anticoagulant (DOAC) that works by directly inhibiting the active site of factor Xa (FXa). Rivaroxaban is metabolized and cleared via the kidney and liver. The results of various studies have shown that patients with severe renal impairment should...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz044

    authors: Milito C,McRae H,Victor A,Refaai MA,Schmidt AE

    更新日期:2020-03-10 00:00:00

  • Current Practice and Regional Variability in Recommendations for Patient Preparation for Laboratory Testing in Primary Care.

    abstract:BACKGROUND:Preparation of the patient for laboratory tests is crucial. Our aim was to investigate the current practice and regional variability of recommendations regarding patient preparation for laboratory testing. METHODS:A call for data was posted by email. Spanish laboratories were invited to fill out and submit ...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz092

    authors: Salinas M,López-Garrigós M,Flores E,Leiva-Salinas C

    更新日期:2020-05-06 00:00:00

  • Ficin-Treated Red Cells Help Identify Clinically Significant Alloantibodies Masked as Reactions of Undetermined Specificity in Gel Microtubes.

    abstract::Non-specific antibodies or antibodies of undetermined significance (AUS) often pose problems for a blood bank technologist and physician. It is well known that antibodies can weaken and evanesce over time, thus eluding detection by routine blood bank techniques. Special enhancement techniques exist (eg, ficin treatmen...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw062

    authors: Hill BC,Hanna CA,Adamski J,Pham HP,Marques MB,Williams LA 3rd

    更新日期:2017-02-01 00:00:00

  • Developmental Defects Associated With DNA Copy Number Gain of Chromosome 2q33.1: A Case Report and Review of Literature.

    abstract::Caspases play a vital role during apoptosis. In addition to apoptosis, caspases play a role in cytokine gene induction and work to inhibit apoptosis. In order for individuals to thrive with useful tissue growth, the rate of cell growth and division must surpass the rate of cell division. It is well established that ex...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx086

    authors: Gupta A,Yo J,Huang G,Soong L,Dong J

    更新日期:2018-03-21 00:00:00

  • ABL Kinase Domain Mutations in Iranian Chronic Myeloid Leukemia Patients with Resistance to Tyrosine Kinase Inhibitors.

    abstract:OBJECTIVE:Tyrosine kinase inhibitors (TKIs) are considered standard first-line treatment in patients with chronic myeloid leukemia. Because ABL kinase domain mutations are the most common causes of treatment resistance, their prevalence and assessment during treatment may predict subsequent response to therapy. METHOD...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmaa052

    authors: Shojaei M,Rezvani H,Azarkeivan A,Poopak B

    更新日期:2020-08-21 00:00:00

  • Rhizobium Radiobacter Infection in a 27-Year-Old African American Woman With Munchausen Syndrome.

    abstract::Rhizobium radiobacter is an opportunistic, usually saprophytic, gram-negative bacillus found in agricultural soil. Isolation from blood has been reported most often in hospitalized patients harboring malignant neoplasms or human immunodeficiency virus (HIV) associated immunosuppression, who have catheter or medical de...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw019

    authors: Sawhney S,Naab T,Oneal P

    更新日期:2016-08-01 00:00:00

  • Mutation Analysis Using Multiplex Ligation-Dependent Probe Amplification in Consanguineous Families in South India with a Child with Profound Hearing Impairment.

    abstract:BACKGROUND:Consanguineous marriage, a common practice in South India, increase the incidence of autosomal recessive diseases such as nonsyndromic hearing loss (NSHL) in offspring. This trend was noted in the children with hearing impairment (HI) who received cochlear implants (CI) at our University hospital in Porur, C...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmz027

    authors: Arunachalam RK,Koshy T,Venkatesan V,Dawson GP,Franklin Durairaj Paul S,George P

    更新日期:2020-01-02 00:00:00

  • Auramine-phenol vs. modified Kinyoun's acid-fast stains for detection of coccidia parasites.

    abstract:OBJECTIVE:Auramine-phenol stain was compared with Kinyoun's acid-fast stain to detect coccidia parasites in fecal samples from immunocompromised patients. The comparison was based on the number of detected cases, sensitivity, specificity, time required for the procedure, ease of use, interpretation, and cost. METHODS:...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/lms0wn7k7ukzcpmi

    authors: Abou El-Naga IF,Gaafar MR

    更新日期:2014-01-01 00:00:00

  • Laboratory Integration and Consolidation in a Regional Health System.

    abstract::Health systems face intense pressure to decrease costs and improve services as the health care delivery system in the United States undergoes tremendous change due to health care reform. As health systems grow, like any business, they are forced to explore standardization to realize and maintain efficient practices. C...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmw069

    authors: Cook J

    更新日期:2017-08-01 00:00:00

  • Impact of Time Lapse on ASCP Board of Certification Medical Laboratory Scientist (MLS) and Medical Laboratory Technician (MLT) Examination Scores.

    abstract:BACKGROUND:Research in several professional fields has demonstrated that delays (time lapse) in taking certification examinations may result in poorer performance by examinees. Thirteen states and/or territories require licensure for laboratory personnel. A core component of licensure is passing a certification exam. A...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/LMNM534LIACPBZWH

    authors: ASCP Board of Certification Research and Development Committee.,Brown KA,Fenn JP,Freeman VS,Fisher PB,Genzen JR,Goodyear N,Houston ML,O'Brien ME,Tanabe PA

    更新日期:2015-07-01 00:00:00

  • The possible advantages of cryoprecipitate prepared from fresh frozen plasma from blood stored for 24 hours.

    abstract:BACKGROUND:To compare the coagulation-factor profile of cryoprecipitate produced from fresh frozen plasma from whole blood (WB) stored for 24 hours at room temperature (24CP) with that of standard cryoprecipitate (CP). METHODS:We collected 80 units of WB from healthy volunteers, of which 20 units were of each blood gr...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1309/lmv1e84uctrqqzp

    authors: Philip J,Kumarage S,Chatterjee T,Kumar S,Mallhi R

    更新日期:2014-04-01 00:00:00

  • Analytical Performance of 10 High-Volume Clinical Chemistry Assays on the Alinity c System.

    abstract:Background:Early access for routine testing with the Alinity c clinical chemistry system (Abbot Laboratories) presented the opportunity to characterize the analytical performance of multiple analytes across clinical laboratories in Europe. Methods:A total of 8 laboratories from 7 European countries evaluated 10 high-v...

    journal_title:Laboratory medicine

    pub_type: 杂志文章,多中心研究

    doi:10.1093/labmed/lmy053

    authors: Krintus M,Fernandez JA,Chesters C,Colla R,Ford C,Frattolillo D,Köller U,Mairesse J,Jimenez DM,Motol J,Padmore K,Sharrod-Cole H,Sypniewska G

    更新日期:2019-01-01 00:00:00

  • Serum Levels of IL-6, IL-8, TNF-α, and TGF-β in Chronic HBV-Infected Patients: Effect of Depression and Anxiety.

    abstract:Objective:To assess the effects of depression and anxiety on serum cytokine levels in patients with chronic hepatitis B (CHB) infection. Methods:In this cross-sectional study, 60 healthy control individuals and 60 patients with CHB participated after filling out standard questionnaires. We examined their serum interle...

    journal_title:Laboratory medicine

    pub_type: 杂志文章

    doi:10.1093/labmed/lmx064

    authors: Bahramabadi R,Fathollahi MS,Hashemi SM,Arababadi AS,Arababadi MS,Yousefi-Daredor H,Bidaki R,Khaleghinia M,Bakhshi MH,Yousefpoor Y,Torbaghan YE,Arababadi MK

    更新日期:2017-12-22 00:00:00