Abstract:
OBJECTIVE:Inflammation of the small intestine may occur in type 2 diabetes. This study aimed to investigate whether ATP-binding cassette transporter A1 (ABCA1) and G1 (ABCG1) were altered in chronic inflammation of the small intestine of type 2 diabetic rats. METHODS:Thirty-two male Sprague-Dawley rats were used. Eight rats in the control group were fed with regular chow, and 24 rats were fed a high-fat diet and injected with a single low dose of streptozotocin. All of the control rats and diabetic rats were bred for 10 months. Immunohistochemistry detected ABCA1 and ABCG1 in the small intestine in all the rats. RESULTS:Hematoxylin-eosin staining showed chronic inflammation in the small intestine of the diabetic rats. Immunohistochemistry staining showed that alteration of ABCA1 and ABCG1 was different in the inflammatory and epithelial cells. Quantitative analysis showed that the overall expression of ABCA1 and ABCG1 increased in the diabetic rats compared to the control rats. Both ABCA1 and ABCG1 were enriched in the inflammatory cells of the small intestine in diabetic rats. In the epithelial cells, ABCA1, but not ABCG1, was detected in significantly more diabetic rats than control rats. CONCLUSION:Both ABCA1 and ABCG1 are enriched in chronic inflammation of the small intestine of type 2 diabetic rats. ABCA1, but not ABCG1, is activated in the intestinal epithelial cells of type 2 diabetic rats.
journal_name
Lab Medjournal_title
Laboratory medicineauthors
Lou J,Zhou H,Li C,Hu L,Lu X,Li J,Yao H,Li W,Zhang X,Xu Mdoi
10.1309/lmo485spyxbqanxjsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
17-24issue
1eissn
0007-5027issn
1943-7730journal_volume
45pub_type
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journal_title:Laboratory medicine
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更新日期:2018-07-05 00:00:00
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更新日期:2018-07-05 00:00:00
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更新日期:2017-12-22 00:00:00
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更新日期:2017-02-01 00:00:00
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更新日期:2017-11-08 00:00:00
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pub_type: 杂志文章
doi:10.1093/labmed/lmw008
更新日期:2016-05-01 00:00:00
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更新日期:2019-10-10 00:00:00
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pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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更新日期:2019-10-10 00:00:00
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journal_title:Laboratory medicine
pub_type: 杂志文章
doi:10.1309/lms0wn7k7ukzcpmi
更新日期:2014-01-01 00:00:00