Comparison of 2 Anti-PLA2R Immunoassays for the Diagnosis of Primary Membranous Nephropathy.

Abstract:

Background:Anti-phospholipase A2 receptor (anti-PLA2R) is a promising biomarker for diagnosis, activity evaluation, therapy monitoring, and prognostic estimation of primary membranous neuropathy (pMN). Difference in methodology may be one cause of the discrepancy in anti-PLA2R-positive percentages in reported studies. In this study, we evaluated the determination consistency of anti-PLA2R using indirect immunofluorescence assay (IIF) and enzyme-linked immunosorbent assay (ELISA). Methods:A total of 113 patients with pMN, 34 with secondary membranous neuropathy (sMN), and 53 healthy control individuals were enrolled. We tested their circulating anti-PLA2R, along with other biochemical parameters, using IIF and ELISA. Results:The sensitivity of anti-PLA2R for pMN was 70.8% by IIF and 67.3% by ELISA, with no statistically significant difference. The overall qualitative agreement of anti-PLA2R was 91.15% (95% confidence interval [CI], 85.91%-96.39%), and the correlation coefficient was 0.79 for IIF versus ELISA. The overall correlation coefficient of anti-PLA2R titers by IIF and concentration by ELISA was 0.85. Through ROC curve analysis, anti-PLA2R as measured by IIF and ELISA showed larger areas under the curve (AUCs) than other biochemical parameters. Conclusion:ELISA shows similar performance to IIF, with the advantages of quantitative results and suitability for high throughput.

journal_name

Lab Med

journal_title

Laboratory medicine

authors

Li W,Guo Y,Zhang Z,Zhang F,Liu X,Ji X,Liu L,Wang H

doi

10.1093/labmed/lmy016

subject

Has Abstract

pub_date

2018-10-11 00:00:00

pages

316-322

issue

4

eissn

0007-5027

issn

1943-7730

pii

4982562

journal_volume

49

pub_type

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